EN ISO 17353:2005

SLOVENSKI STANDARD
01-december-2005
.DNRYRVWYRGH±'RORþHYDQMHL]EUDQLKRUJDQRNRVLWUQLKVSRMLQ0HWRGDSOLQVNH
NURPDWRJUDILMH,62
Water quality - Determination of selected organotin compounds - Gas chromatographic
method (ISO 17353:2004)
Wasserbeschaffenheit - Bestimmung von ausgewählten Organozinnverbindungen -
Verfahren mittels Gaschromatographie (ISO 17353:2004)
Qualité de l'eau - Dosage de composés organostanniques sélectionnés - Méthode par
chromatographie en phase gazeuse (ISO 17353:2004)
Ta slovenski standard je istoveten z: EN ISO 17353:2005
ICS:
13.060.50 3UHLVNDYDYRGHQDNHPLþQH Examination of water for
VQRYL chemical substances
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 17353
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2005
ICS 13.060.50
English Version
Water quality - Determination of selected organotin compounds -
Gas chromatographic method (ISO 17353:2004)
Qualité de l'eau - Dosage de composés organostanniques Wasserbeschaffenheit - Bestimmung von ausgewählten
sélectionnés - Méthode par chromatographie en phase Organozinnverbindungen - Verfahren mittels
gazeuse (ISO 17353:2004) Gaschromatographie (ISO 17353:2004)
This European Standard was approved by CEN on 18 July 2005.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.
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Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17353:2005: E
worldwide for CEN national Members.

Foreword
The text of ISO 17353:2004 has been prepared by Technical Committee ISO/TC 147 "Water quality" of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 17353:2005 by
Technical Committee CEN/TC 230 “Water analysis”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2006, and conflicting national standards shall be withdrawn
at the latest by February 2006.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic,
Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland
and United Kingdom.
Endorsement notice
The text of ISO 17353:2004 has been approved by CEN as EN ISO 17353:2005 without any modifications.
INTERNATIONAL ISO
STANDARD 17353
First edition
2004-09-15
Water quality — Determination of
selected organotin compounds — Gas
chromatographic method
Qualité de l'eau — Dosage de composés organostanniques
sélectionnés — Méthode par chromatographie en phase gazeuse

Reference number
ISO 17353:2004(E)
©
ISO 2004
ISO 17353:2004(E)
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ii © ISO 2004 – All rights reserved

ISO 17353:2004(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope. 1
2 Normative references . 1
3 Terms and definitions. 2
4 Principle . 2
5 Interferences. 2
6 Reagents . 2
7 Apparatus. 7
8 Procedure. 8
9 Identification. 11
10 Calibration. 13
11 Calculation. 16
12 Precision . 18
13 Test report. 18
Annex A (informative) Gas chromatographic conditions. 19
Annex B (informative) Precision data. 29
Bibliography . 31

ISO 17353:2004(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 17353 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2, Physical,
chemical and biochemical methods.
iv © ISO 2004 – All rights reserved

ISO 17353:2004(E)
Introduction
It should be noted whether and to what extent particular problems will require the specification of additional
boundary conditions.
This International Standard describes a gas-chromatographic/organotin specific determination of organotin
compounds after derivatization with sodium tetraethyl borate and liquid/liquid extraction.
The user should be aware that particular problems could require the specification of additional marginal
conditions.
INTERNATIONAL STANDARD ISO 17353:2004(E)

Water quality — Determination of selected organotin
compounds — Gas chromatographic method
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This International Standard does not purport to address all of the safety problems, if any,
associated with its use. It is the responsibility of the user to establish appropriate safety and health
practices and to ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted according to this International Standard
be carried out by suitably trained staff.
1 Scope
This International Standard specifies a method for the identification and quantification of organotin compounds
and/ or cations as mentioned in Table 1 in drinking water, surface water and wastewater containing not more
than 2 g/l of suspended material. The working range is 10 ng/l to 1 000 ng/l. The respective anions are not
determined.
This method can also be applicable to other compounds such as R = methyl, n = 1 to 2 and R = phenyl, n = 1
to 2. This International Standard is also applicable to marine water.
Table 1 — Organotin compounds and cations determined
using this International Standard
(4-n)+
R Sn
R n Name Acronym
n
3+
BuSn Butyl 1 Monobutyltin cation MBT
2+
Bu Sn
Butyl 2 Dibutyltin cation DBT
+
Bu Sn
Butyl 3 Tributyltin cation TBT
Bu Sn
Butyl 4 Tetrabutyltin TTBT
3+
Octyl 1 Monooctyltin cation MOT
OcSn
2+
Oc Sn Octyl 2 Dioctyltin cation DOT
+
Ph Sn
Phenyl 3 Triphenyltin cation TPhT
+
Cy Sn
Cyclohexyl 3 Tricyclohexyltin cation TCyT
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods
ISO 17353:2004(E)
ISO 5667-1, Water quality — Sampling — Part 1: Guidance on the design on sampling programmes
ISO 5667-2, Water quality — Sampling — Part 2: Guidance on sampling techniques
ISO 5667-3, Water quality — Sampling — Part 3: Guidance on the preservation and handling of water
samples
3 Terms and definitions
For the purposes of this International Standard, the following terms and definitions apply.
3.1
organotin compound
OTC
substance with at least one Sn-C bond
NOTE The number of Sn-C bonds is a measure of the degree of substitution.
3.2
organotin cation
OC
part of the organotin compound that contains all Sn-C bonds (and which is formally loaded)
NOTE In this International Standard, the abbreviation OC is also used for the non-dissociated tetrasubstituted
organotin. OC therefore comprises the cations MBT, DBT, TBT, TTBT, MOT, DOT, TCyT, and TPhT.
4 Principle
Organotin compounds in water are alkylated with sodium tetraethylborate and extracted with hexane. The
extract can be cleaned with silica. After concentration, the tetrasubstituted OTC are separated by capillary gas
chromatography and detected with a suitable system such as MS (mass spectrometry), FPD (flame
photometric detection), AED (atomic emission detection). The concentration is determined by calibration for
the total procedure using an internal standard mixture.
5 Interferences
The reagents sometimes contain impurities of organotin compounds. It is absolutely essential to verify the
blanks (see A.4.6).
6 Reag
...