EN 46-2:2006

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Wood preservatives - Determination of the preventive action against Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (laboratory method)ODERUDWRULMVNDPHWRGDProduits de préservation du bois - Détermination de l'action préventive contre Hylotrupes bajulus (Linnaeus) - Partie 2 : Effet ovicide (Méthode de laboratoire)Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Hylotrupes bajulus (Linnaeus) - Teil 2: Ovizide Wirkung (Laboratoriumsverfahren)Ta slovenski standard je istoveten z:EN 46-2:2006SIST EN 46-2:2006en71.100.50ICS:SLOVENSKI
STANDARDSIST EN 46-2:200601-julij-2006

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 46-2March 2006ICS 71.100.50 English VersionWood preservatives - Determination of the preventive actionagainst Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect(laboratory method)Produits de préservation du bois - Détermination de l'actionpréventive contre Hylotrupes bajulus (Linnaeus) - Partie 2 :Effet ovicide (Méthode de laboratoire)Holzschutzmittel - Bestimmung der vorbeugenden Wirkunggegenüber Hylotrupes bajulus (Linnaeus) - Teil 2: OvizideWirkung (Laboratoriumsverfahren)This European Standard was approved by CEN on 23 January 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2006 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 46-2:2006: E

test specimens .8 7.4 Dimensions of test specimens.8 7.5 Number of test specimens.8 7.5.1 Test specimens for egg-laying.8 7.5.2 Test specimens for checking the tunnelling ability and the mortality of the larvae.8 8 Procedure.8 8.1 Preparation of the test specimens.8 8.1.1 Conditioning of the test specimens prior to sealing.8 8.1.2 Sealing of the transverse faces.9 8.1.3 Treatment of the test specimens.9 8.1.4 Drying and conditioning of the test specimens after treatment.10 8.2 Exposure of the test specimens to the insects (ovicidal action).11 8.3 Validity of the test.11 9 Expression of results.11 9.1 Ovicidal test.11 9.2 Tunnelling control.11 9.3 Total mortality.12 10 Test report.12 Annex A (informative) Example of a test report.14 Hatching ability.15 Tunnelling ability.15 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus).17 Annex C (informative) Environmental, health and safety precautions within chemical/biological laboratory.20 Bibliography.21

 water-insoluble chemicals which are being studied as active insecticides,  organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates,  organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates, or  water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references the latest edition of the referenced document (including any amendments) applies. EN 46-1, Wood preservatives - Determination of the preventive action against Hylotrupes bajulus (Linnaeus) - Part 1:Larvicidial effect (Laboratory method) EN 73, Wood preservatives - Accelerated ageing of treated wood prior to biological testing - Evaporative ageing procedure EN 84, Wood preservatives - Accelerated ageing of treated wood prior to biological testing - Leaching procedure EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987) 3 Terms and definitions For the purposes of this European Standard, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled [EN 1001-2:2005, 4.71] 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested)

5.1.1 Hylotrupes bajulus (Linnaeus) females 5.1.2 Source of females The insects shall preferably be obtained from cultures reared as e.g. described in annex B. Use only sound and lively insects.
5.2 Products and reagents
5.2.1 Paraffin wax, for fixing the glass plate in all cases and for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable. 5.2.2 Gelatin, for sealing the end faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION — Do not use benzene or other solvents which pose a health risk. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 ± 2) °C and at relative humidity (70 ± 5) %. 5.3.2 Conditioning chamber, well ventilated and controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %. NOTE The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). 5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. CAUTION — It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours.

The wood shall be free from visible cracks, stain, decay, insect damage and other defects.
The wood shall not have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees preferably felled in winter. The wood shall not have been stored for more than five years. NOTE 1 Wood that has been kiln dried at temperatures below 60 °C may be used. The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole. NOTE 2 It is recommended to use test specimens of similar growth rate within a single test.
1) In southern European countries the pine species most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this standard has been demonstrated in all aspects (development of larvae, resistance of impregnation etc…).

test specimens (2 of each subset, see 7.3) for each preservative concentration or retention to be tested. - three control test specimens (1 of each subset, see 7.3) for each test series, treated with the preservative containing no active ingredients. - three untreated control test specimens (1 of each subset, see 7.3). 7.5.2 Test specimens for checking the tunnelling ability and the mortality of the larvae In addition to the test specimens for egg-laying at least six test specimens shall be prepared for each preservative concentration and retention for checking the tunnelling ability and the mortality of the newly hatched larvae. 8 Procedure 8.1 Preparation of the test specimens 8.1.1 Conditioning of the test specimens prior to sealing Allow the test specimens to condition in the conditioning chamber (5.3.2) for a minimum of two weeks.
2) For special tests, test specimens may be obtained according to a given series. As a result, it may be preferable to take test specimens from pretreated strips.

test specimens in the conditioning chamber (5.3.2) for at least one day. 8.1.3 Treatment of the test specimens 8.1.3.1 Preparation of the treatment solutions 8.1.3.1.1 Solid preservative
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