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CEN/TR 15214-2:2006

(Main)

Characterization of sludges - Detection and enumeration of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes - Part 2: Miniaturised method (Most Probable Number) by inoculation in liquid medium

Characterization of sludges - Detection and enumeration of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes - Part 2: Miniaturised method (Most Probable Number) by inoculation in liquid medium

This part of the CEN Technical Report describes a miniaturized most probable number (MPN) method for the quantitative detection of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes. It is suitable to evaluate the log reduction of E. coli through treatment as well as the quality of the end product.
This method is convenient for material with dry residue of more than 10 %. For materials with dry residue less than 20 %, the procedure specified in CEN/TR 15214-1 will be used.

Charakterisierung von Schlämmen - Quantitativer Nachweis von Escherichia coli in Schlämmen, Böden, Bodenverbesserungsmitteln, Kultursubstraten sowie Bioabfällen - Teil 2: Miniaturisiertes Verfahren durch Animpfen in Flüssigmedium (MPN-Verfahren)

Dieses Dokument legt ein miniaturisiertes Verfahren der wahrscheinlichsten Keimzahl (MPN-Verfahren) für den quantitativen Nachweis von Escherichia coli in Schlämmen, Böden, Bodenverbessererungsmitteln, Kultursubstraten und Bioabfall fest. Es eignet sich für die Bewertung der logarithmischen Abnahme von E. coli durch Behandlung sowie der Qualität des Endproduktes.
Das vorliegende Verfahren ist für Materialien mit einem Trockenrückstand von mehr als 10 % geeignet. Bei Materialien mit einem Trockenrückstand von weniger als 20 % wird das in CEN/TR 15214-1 festgelegte Verfahren angewendet.

Caractérisation des boues - Détection et dénombrement des Escherichia coli dans les boues, les sols, les amendements du sol, les supports de culture et les biodéchets - Partie 2 : Méthode miniaturisée (nombre le plus probable) par ensemencement en milieu liquide

La présente partie du Rapport Technique CEN décrit une méthode miniaturisée (nombre le plus probable – NPP) de détection quantitative d’Escherichia coli dans les boues, les sols, les amendements du sol, les supports de culture et les biodéchets. Cette méthode convient pour évaluer la réduction log d'Escherichia coli pendant le traitement, ainsi que la qualité du produit fini.
Cette méthode s’applique aux matériaux ayant une teneur en matière sèche supérieure à 10 %. Le mode opératoire spécifié dans le CEN/TR 15214-2 sera utilisé lorsque les matériaux ont une teneur en matière sèche inférieure à 20 %.

Karakterizacija blata – Ugotavljanje prisotnosti in števila Echerichia coli v blatu, zemljini, izboljševalcih tal, rastnih substratih in bio-odpadkih - 2. del: Miniaturizirana metoda (najverjetnejše število – MPN) s cepljenjem v tekoči medij

General Information

Status
Published
Publication Date
24-Jan-2006
ICS
07.100.99 - Other standards related to microbiology
Technical Committee
CEN/TC 308 - Characterization of sludges
Drafting Committee
CEN/TC 308/WG 1 - Characterization methods
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
25-Jan-2006
Completion Date
25-Jan-2006
Ref Project
SIST-TP CEN/TR 15214-2:2006 - Characterization of sludges - Detection and enumeration of Escherichia coli in sludges, soils, soil improvers, growing media and biowastes - Part 2: Miniaturised method (Most Probable Number) by inoculation in liquid medium

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SLOVENSKI STANDARD
SIST-TP CEN/TR 15214-2:2006
01-julij-2006
.DUDNWHUL]DFLMDEODWD±8JRWDYOMDQMHSULVRWQRVWLLQãWHYLOD(FKHULFKLDFROLYEODWX
]HPOMLQLL]EROMãHYDOFLKWDOUDVWQLKVXEVWUDWLKLQELRRGSDGNLKGHO
0LQLDWXUL]LUDQDPHWRGD QDMYHUMHWQHMãHãWHYLOR±031 VFHSOMHQMHPYWHNRþLPHGLM
Characterization of sludges - Detection and enumeration of Escherichia coli in sludges,
soils, soil improvers, growing media and biowastes - Part 2: Miniaturised method (Most
Probable Number) by inoculation in liquid medium
Charakterisierung von Schlämmen - Quantitativer Nachweis von Escherichia coli in
Schlämmen, Böden, Bodenverbesserungsmitteln, Kultursubstraten sowie Bioabfällen -
Teil 2: Miniaturisiertes Verfahren durch Animpfen in Flüssigmedium (MPN-Verfahren)
Caractérisation des boues - Détection et dénombrement des Escherichia coli dans les
boues, les sols, les amendements du sol, les supports de culture et les biodéchets -
Partie 2 : Méthode miniaturisée (nombre le plus probable) par ensemencement en milieu
liquide
Ta slovenski standard je istoveten z: CEN/TR 15214-2:2006
ICS:
13.030.20
13.080.30
65.080
SIST-TP CEN/TR 15214-2:2006 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

TECHNICAL REPORT
CEN/TR 15214-2
RAPPORT TECHNIQUE
TECHNISCHER BERICHT
January 2006
ICS 07.100.99

English Version
Characterization of sludges - Detection and enumeration of
Escherichia coli in sludges, soils, soil improvers, growing media
and biowastes - Part 2: Miniaturised method (Most Probable
Number) by inoculation in liquid medium
Caractérisation des boues - Détection et dénombrement Charakterisierung von Schlämmen - Quantitativer
des Escherichia coli dans les boues, les sols, les engrais, Nachweis von Escherichia coli in Schlämmen, Böden,
les amendements organiques et les biodéchets - Partie 2 : Düngemitteln und Bodenverbesserern, Kultursubstraten
Méthode miniaturisée (nombre le plus probable) par sowie Bioabfällen - Teil 2: Miniaturisiertes Verfahren durch
ensemencement en milieu liquide Animpfen in Flüssigmedium (MPN-Verfahren)
This Technical Report was approved by CEN on 3 September 2005. It has been drawn up by the Technical Committee CEN/TC 308.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2006 CEN All rights of exploitation in any form and by any means reserved Ref. No. CEN/TR 15214-2:2006: E
worldwide for CEN national Members.

---------------------- Page: 2 ----------------------

CEN/TR 15214-2:2006 (E)
Contents Page
Foreword .3
Introduction.4
1 Scope .5
2 Normative references .5
3 Terms and definitions.5
4 Principle.6
5 Apparatus .6
6 Sampling and hazards .7
6.1 Introduction.7
6.2 General .7
6.3 Storage .7
6.4 Handling.7

7 Reagents, diluents and culture media.
...

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