EN 49-1:2005

SLOVENSKI STANDARD
01-julij-2005
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Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 1: Application by surface
treatment (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 1: Oberflächenbehandlung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis a vis d'
Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des
larves - Partie 1 : Application par traitement de surface (Méthode de laboratoire)
Ta slovenski standard je istoveten z: EN 49-1:2005
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN 49-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
March 2005
ICS 71.100.50 Supersedes EN 49-1:1992
English version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by egg-
laying and larval survival - Part 1: Application by surface
treatment (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden Wirkung
l'efficacité protectrice vis à vis d' Anobium punctatum (De gegenüber Anobium punctatum (De Geer) durch
Geer) par l'observation de la ponte et du taux de survie des Beobachten der Eiablage und des Überlebens von Larven -
larves - Partie 1: Application par traitement de surface Teil 1: Oberflächenbehandlung (Laborverfahren)
(Méthode de laboratoire)
This European Standard was approved by CEN on 3 February 2005.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the Central Secretariat or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official
versions.
CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,
Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Slovakia,
Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2005 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 49-1:2005: E
worldwide for CEN national Members.

Contents Page
Foreword.3
Introduction .4
1 Scope .5
2 Normative references .5
3 Terms and definitions .5
4 Principle.5
5 Test materials.6
6 Sampling.7
7 Test specimens.7
8 Procedure .8
9 Validity of test .11
10 Expression of results .11
11 Test report .12
Annex A (informative) Example of a test report .14
Annex B (informative) Identification of sex of test insects Anobium punctatum.16
Annex C (informative) Culturing technique for Anobium punctatum .17
Annex D (informative) Environmental, health and safety precautions within chemical/biological
laboratory .20
Bibliography .21

Foreword
This document (EN 49-1:2005) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and
wood-based products”, the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical text or
by endorsement, at the latest by September 2005, and conflicting national standards shall be withdrawn at the
latest by September 2005.
This document supersedes EN 49-1:1992.
This document consists of two parts, Part 1 is required to enable effectiveness assessments of wood preservatives
which are intended to be applied by surface treatment and Part 2 those which are intended to be applied by
impregnation.
Significant technical differences between this document and EN 49-1:1992 are as follows:
a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests;
b) acknowledgement of the terms given in EN 1001-1;
c) introduction of an informative Annex to take account of consideration for minimisation of environmental and
health hazards caused by the use of this biological test.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark,
Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the effectiveness of
a wood preservative, when applied as a surface treatment, against Anobium punctatum. It allows the determination
of the concentration at which the product prevents the development of infestation from egg laying. It can also be
used with formulations ready for use.
The method simulates conditions which can occur in practice on timber which has been treated some time
previously with wood preservative applied by dip, brush or spray and on which eggs of Anobium punctatum are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In making this
assessment the methods by which the preservative may be applied should be taken into account. It is further
recommended that results from this test should be supplemented by those from other appropriate tests, and above
all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take suitable precautions
to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood,
laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within
rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D for
environmental, health and safety precautions).

1 Scope
This document specifies a method for the determination of the protective effectiveness or the toxic values of a
wood preservative against infestation by Anobium punctatum (De Geer) when the product is applied as a surface
treatment to wood.
This method is applicable to:
 water-insoluble chemicals which are being studied as active insecticides,
 organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates,
 organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates,
and
 water-soluble materials, for example salts.
NOTE This method may be used in conjunction with an ageing procedure, for example EN 73.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references,
only the edition cited applies. For undated references the latest edition of the referenced document (including any
amendments) applies.
EN ISO 3696, Water for analytical laboratory use – Specification and test methods (ISO 3696:1987)
ISO 835-1, Laboratory glassware – Graduated pipettes – Part 1: General requirements
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total
volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
Depending on the test being carried out either:
 on a set of test specimens of a susceptible wood species that is surface treated with a solution of the
preservative, or
 if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are
surface treated with a series of solutions in which the concentration of preservative is ranged in a given
progression.
The treated test specimens are exposed to gravid females of Anobium punctatum. The number of eggs laid, the
number of eggs hatched and the numbers of surviving larvae are observed and compared with those in untreated
control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or by
dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test
specimens.
5 Test materials
5.1 Biological material
Anobium punctatum (De Geer)
Adult males and females in good condition.
Adults to be used in the test shall be collected at daily intervals from naturally infested wood or laboratory culture
(see Annex C).
Use recently emerged adults which have been recently collected; kept overnight in quarantine (see C.6); and then
checked to ensure that they are undamaged, active and free from any infestation by mites. Determine the sex
(see Annex B) of the collected and checked adults and place the males and females in separate containers.
NOTE The proportion of males and females varies during the emergence period.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the relevant faces of test specimens to be treated with solutions in which water is
the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found to be suitable.
5.2.2 Gelatin, for sealing the relevant faces of test specimens to be treated with solutions in which an organic
solvent is the continuous phase.
5.2.3 Paste, for securing filter paper. The paste shall be starch-free, non-toxic to Anobium punctatum and
insoluble in the product under test.
NOTE Sodium carboxy methyl cellulose, food grade, has been found to be suitable.
5.2.4 Water, complying with grade 3 of EN ISO 3696.
5.2.5 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue
in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects.
CAUTION — Do not use benzene or other solvents which pose a health risk.
5.2.6 Filter paper, ordinary quality, medium-fast grade.
5.2.7 Fine cloth, of cotton or linen, with a mesh aperture of 0,3 mm to 0,6 mm.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (21 ± 2) °C, and at relative humidity (80 ± 5) %.
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and at relative humidity (65 ± 5) %.
NOTE The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has
the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Treatment vessel, of a material that does not react with the pres
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