EN ISO 10273:2017

SLOVENSKI STANDARD
01-julij-2017
1DGRPHãþD
SIST EN ISO 10273:2003
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje
prisotnosti patogene bakterije Yersinia enterocolitica (ISO 10273:2017)
Microbiology of the food chain - Horizontal method for the detection of pathogenic
Yersinia enterocolitica (ISO 10273:2017)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis von
pathogenen Yersinia enterocolitica (ISO 10273:2017)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour la recherche de
Yersinia enterocolitica pathogènes (ISO 10273:2017)
Ta slovenski standard je istoveten z: EN ISO 10273:2017
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 10273
EUROPEAN STANDARD
NORME EUROPÉENNE
April 2017
EUROPÄISCHE NORM
ICS 07.100.30 Supersedes EN ISO 10273:2003
English Version
Microbiology of the food chain - Horizontal method for the
detection of pathogenic Yersinia enterocolitica (ISO
10273:2017)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour la recherche de Yersinia Verfahren zum Nachweis von pathogenen Yersinia
enterocolitica pathogènes (ISO 10273:2017) enterocolitica (ISO 10273:2017)
This European Standard was approved by CEN on 13 March 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 10273:2017 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 10273:2017) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by October 2017, and conflicting national standards shall
be withdrawn at the latest by October 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 10273:2003.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 10273:2017 has been approved by CEN as EN ISO 10273:2017 without any modification.

INTERNATIONAL ISO
STANDARD 10273
Third edition
2017-03
Microbiology of the food chain —
Horizontal method for the detection
of pathogenic Yersinia enterocolitica
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
la recherche de Yersinia enterocolitica pathogènes
Reference number
ISO 10273:2017(E)
©
ISO 2017
ISO 10273:2017(E)
© ISO 2017, Published in Switzerland
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ii © ISO 2017 – All rights reserved

ISO 10273:2017(E)
Contents Page
Foreword .v
Introduction .vii
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 2
5 Principle . 2
5.1 General . 2
5.2 Direct plating from liquid enrichment medium . 2
5.3 Enrichment in liquid enrichment medium and selective liquid enrichment medium . 2
5.4 Plating out after enrichment and identification . 2
5.5 Confirmation . 3
6 Culture media and reagents . 3
7 Equipment and consumables . 3
8 Sampling . 3
9 Preparation of test sample . 4
Procedure (as shown in Annex A) . 4
10.1 Test portion and initial suspension . 4
10.2 Direct plating on selective agar . 4
10.3 Enrichment . 5
10.4 Plating out and incubation of plates . 5
10.4.1 Plating from PSB and ITC by KOH treatment on CIN agar. 5
10.4.2 Plating from PSB and ITC by KOH treatment on chromogenic agar (optional) . 5
10.5 Identification of characteristic colonies . 5
10.6 Confirmation . 6
10.6.1 General. 6
10.6.2 Selection of colonies for confirmation . 6
10.6.3 Determination of pathogenic Yersinia species . 6
10.6.4 Confirmation of pathogenic Y. enterocolitica . 8
10.6.5 Interpretation of confirmation tests for Y. enterocolitica.10
10.6.6 Interpretation of confirmation tests for pathogenic Y. enterocolitica .10
10.7 Biotyping of Y. enterocolitica (optional) .10
10.7.1 General.10
10.7.2 Fermentation of xylose .11
10.7.3 Tween-esterase test .11
10.7.4 Fermentation of salicin (optional) and trehalose .11
10.7.5 Indole formation .11
10.7.6 Interpretation of biotyping tests .11
11 Expression of results .12
12 Performance characteristics of the method .12
12.1 Interlaboratory study .12
12.2 Sensitivity .12
12.3 Specificity .12
12.4 LOD .12
13 Test report .12
14 Quality assurance .13
Annex A (normative) Diagrams of the procedures .
...