EN ISO 9167-2:1997

SLOVENSKI STANDARD
01-november-1998
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Rapeseed - Determination of glucosinolates content - Part 2: Method using X-ray
fluorescence spectrometry (ISO 9167-2:1994)
Rapssamen - Bestimmung des Glucosinolatgehaltes - Teil 2: Verfahren mit
Röntgenfluoreszenzanalyse (ISO 9167-2:1994)
Graines de colza - Dosage des glucosinolates - Partie 2: Méthode par spectrométrie de
fluorescence aux rayons X (ISO 9167-2:1994)
Ta slovenski standard je istoveten z: EN ISO 9167-2:1997
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

INTERNATIONAL
ISO
STANDARD
9167-2
First edition
1994-10-01
Rapeseed - Determination of
glucosinolates content -
Part 2:
Method using X-ray fluorescence
spectrometry
- Dosage des glucosinolates -
Graines de colza
Partie 2: Methode par spectromb-ie de fluorescence aux rayons X
Reference number
ISO 916721994(E)
ISO 9167=2:1994(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide
federation of national Standards bodies (ISO member bodies). The work
of preparing International Standards is normally carried out through ISO
technical committees. Esch member body interested in a subject for
which a technical committee has been established has the right to be
represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard ISO 9167-2 was prepared by Technical Committee
lSO/TC 34, Agrkultural food products, Subcommittee SC 2, Oleaginous
seeds and fruits.
ISO 9167 consists of the following Parts, under the general title
Rapeseed - Determination o f glucosinola tes con ten t:
- Part 1: Method using high-performance liquid chromatography
- Part 2: Method using X-ray fluorescence spectrometry
Annexes A and B of this part of ISO 9167 are for information only.
0 ISO 1994
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronie or mechanical, including photocopying and
microfilm, without Permission in writing from the publisher.
International Organization for Standardization
Case Postale 56 l CH-l 211 Geneve 20 l Switzerland
Printed in Switzerland
ii
INTERNATIONAL STANDARD 0 ISO
ISO 9167=2:1994(E)
Rapeseed - Determination of glucosinolates
content -
Part 2:
Method using X-ray fluorescence spectrometry
maintain registers of currently valid International
1 Scope
Standards.
This part of ISO 9167 specifies a rapid method for the
ISO 664: 1990, Oilseeds - Reduction of laboratory
determination of the total glucosinolate content of
Sample to test Sample.
rapeseed using X-ray fluorescence spectrometry
(XRF).
ISO 665:1977, Oilseeds - Determination of moisture
and vola tile matter con ten t.
lt is applicable to seeds with a normal Protein content
from 19 % to 23 %. For seeds with a Protein content
outside this range, account should be taken of the
3 Definition
total Protein content in the calculation of the total
glucosinolate content (see reference [4]).
For the purposes of this patt of ISO 9167, the follow-
ing definition applies.
NOTES
1 ISO 9167-1 specifies a method using high-performance
3.1 total glucosinolate content: The total Sulfur
liquid chromatography (HPLC) which enables the content
content minus those amounts that are bound in pro-
of different glucosinolates to be determined individually.
teins or Single glucosinolates that cannot be deter-
mined by direct reference methods, divided by the
2 When applied to immature seed or to seed that has
average stoichiometric number of Sulfur atoms oc-
been badly stored, for example under damp and warm
curring in the glucosinolate fraction typical of Brassica
conditions, the results obtained by the XRF method and by
the HPLC method (ISO 9167-1) may not agree as closely as species.
when applied to normal rapeseed. The HPLC method should
be taken as the reference method.
.
4 Principle
Determination of total Sulfur content in ground and
compressed rapeseed by X-ray fluorescence spec-
2 Normative references
troscopy. Calculation of the glucosinolate content by
comparison with values of reference samples with a
The following Standards contain provisions which,
certified Sulfur content.
through reference in this text, constitute provisions
of this part of ISO 9167. At the time of publication, the
editions indicated were valid. All Standards are subject
5 Materials
to revision, and Parties to agreements based on this
part of ISO 9167 are encouraged to investigate the
possibility of applying the most recent editions of the 5.1 Whole rapeseed reference materials, three
Standards indicated below. Members of IEC and ISO samples, each with a certified Sulfur content.
0 ISO
ISO 9167=2:1994(E)
8.1.2 If the moisture content of the seeds exceeds
5.2 Whole rapeseed secondary reference ma-
10 %, reduce this to between 7 % and 9 % by drying
terials, three samples, each with a Sulfur content
which is traceable to certified whole rapeseed refer- about 40 g (60 ml) of the seeds, spread out in a thin
ence materials (5.1). layer, either in the microwave oven (6.7) for 2 min or
in the ventilated oven for 75 min at 85 “C.
5.3 Synthetic calibration samples, three samples,
Allow the seeds to cool to room temperature before
covering the range of Sulfur content of interest.
proceeding.
NOTE 3 These samples may be those provided by the
manufacturer of the X-ray spectrometer or prepared from
8.1.3 Carry out a parallel determination of the
powdered material.
moisture content in accordance with the method
given in ISO 665. .
6 Apparatus
8.2 Preparation of reference samples
Usual laboratory apparatus and, in particular, the fol-
lowing.
8.2.1 Treat the rapeseed reference materials (5.1)
as described in 8.1.2 and 8.1.3. Three examples of
6.1 Spoon, allowing the taking of about 20 g (about
each reference Sample are required.
30 ml) of seed.
8.2.2 Use these samples to calibrate the apparatus
6.2 Blender, coffee-mill type of 100 cm3 volume,
when it is first put into Service and at each further
8 cm diameter and 180 W power.
calibration (see 9.3.2).
6.3 Spatulas,
8.2.3 Use a new series of reference samples of
rapeseed (5.1) for each new calibration procedure.
6.4 Aluminium cups or liquid cuvettes, with win-
dows of polycarbonate film.
9 Procedure
6.5 Hydraulic press, or other device (hand-press)
allowing repeatable application of a defined pressure.
9.1 Test Po
...