EN 16877:2016
(Main)Animal feeding stuffs: Methods of sampling and analysis - Determination of T-2 and HT-2 toxins, Deoxynivalenol and Zearalenone, in feed materials and compound feed by LC-MS
Animal feeding stuffs: Methods of sampling and analysis - Determination of T-2 and HT-2 toxins, Deoxynivalenol and Zearalenone, in feed materials and compound feed by LC-MS
This method of analysis is applicable to the determination of HT-2 toxin (HT2) in the tested range of 22 µg/kg to 178 µg/kg, T-2 toxin (T2) in the tested range of 7 µg/kg to 50 µg/kg, Deoxynivalenol (DON) in the tested range of 88 µg/kg to 559 µg/kg, and Zearalenone (ZON) in the tested range of 14 µg/kg to 430 µg/kg in cereals and cereal-based compound animal feed. The actual working ranges may extend beyond the tested ranges. It is the responsibility of the laboratory to prove that the limit of quantitation (LOQ) for HT-2 and T-2 toxin is ≤ 10 µg/kg, for DON ≤ 100 µg/kg, and for ZON ≤ 20µg/kg.
Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von T-2- und HT-2-Toxinen, Deoxynivalenol und Zearalenon in Einzelfuttermitteln und Mischfuttermitteln mittels LC-MS
Dieses Analysenverfahren ist für die Bestimmung von HT-2-Toxin (HT2) im geprüften Bereich von 22 µg/kg bis 178 µg/kg, von T-2-Toxin (T2) im geprüften Bereich von 7 µg/kg bis 50 µg/kg, von Deoxynivalenol (DON) im geprüften Bereich von 88 µg/kg bis 559 µg/kg und von Zearalenon (ZON) im geprüften Bereich von 14 µg/kg bis 430 µg/kg in Getreide und in Mischfuttermitteln auf Getreidebasis anwendbar. Die tatsächlichen Arbeitsbereiche dürfen über die geprüften Bereiche hinausgehen. Es liegt in der Verantwortung des Laboratoriums nachzuweisen, dass die Bestimmungsgrenze (LOQ, en: limit of quantitation) für HT-2- und T 2 Toxine ≤ 10 µg/kg, für DON ≤ 100 µg/kg und für ZON ≤ 20 µg/kg ist.
Aliments des animaux - Méthodes d'échantillonnage et d'analyse - Dosage par CL-SM des toxines T-2 et HT-2, du déoxynivalénol et de la zéaralénone dans les matières premières pour aliments et les aliments composés
La présente méthode d'analyse s'applique au dosage de la toxine HT-2 (HT2) dans la gamme validée de 22 µg/kg à 178 µg/kg, de la toxine T-2 (T2) dans la gamme validée de 7 µg/kg à 50 µg/kg, du déoxynivalénol (DON) dans la gamme validée de 88 µg/kg à 559 µg/kg, et de la zéaralénone (ZON) dans la gamme validée de 14 µg/kg à 430 µg/kg, dans les céréales et les aliments composés pour animaux à base de céréales. Les gammes de travail réelles peuvent s'étendre au-delà des gammes validées. Il incombe au laboratoire de démontrer que la limite de quantification (LOQ) est ≤ 10 µg/kg pour les toxines HT-2 et T-2, ≤ 100 µg/kg pour DON et ≤ 20 µg/kg pour ZON.
Krma - Metode vzorčenja in analize - Določevanje toksinov T-2 in HT-2, deoksinivalenola in zearalenona v sestavinah krme in krmni mešanici z LC-MS
Ta metoda analize se uporablja za določevanje toksina HT-2 (HT2) v preskušanem obsegu od 22 µg/kg do 178 µg/kg in toksina T-2 (T2) v preskušanem obsegu od 7 µg/kg do 50 µg/kg v sestavinah krme in krmni mešanici. Dejanski delovni obsegi lahko presegajo preskušane obsege. Laboratorij mora dokazati, da je mejna vrednost za količinsko ovrednotenje (LOQ) za toksina HT-2 in T-2 10 µg/kg ali ustreznejša za posamezen toksin. Ta metoda se uporablja tudi za določevanje deoksinivalenola (DON) v preskušanem obsegu od 88 µg/kg do 559 µg/kg in zearalenona (ZON) v preskušanem obsegu od 14 µg/kg do 430 µg/kg.
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.+7Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von T-2- und HT-2-Toxinen, Deoxynivalenol und Zearalenon in Einzelfuttermitteln und Mischfuttermitteln mittels LC-MSAliments des animaux - Méthodes d'échantillonnage et d'analyse - Dosage par CL-SM des toxines T-2 et HT-2, du déoxynivalénol et de la zéaralénone dans les matières premières pour aliments et les aliments composésAnimal feeding stuffs: Methods of sampling and analysis - Determination of T-2 and HT-2 toxins, Deoxynivalenol and Zearalenone, in feed materials and compound feed by LC-MS71.040.50Fizikalnokemijske analitske metodePhysicochemical methods of analysis65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN 16877:2016SIST EN 16877:2017en,fr,de01-februar-2017SIST EN 16877:2017SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16877
November 2016 ICS 65.120; 71.040.50
English Version
Animal feeding stuffs: Methods of sampling and analysis - Determination of T-2 and HT-2 toxins, Deoxynivalenol and Zearalenone, in feed materials and compound feed by LC-MS
Aliments des animaux - Méthodes d'échantillonnage et d'analyse - Dosage par CL-SM des toxines T-2 et HT-2, du déoxynivalénol et de la zéaralénone dans les matières premières pour aliments et les aliments composés
Futtermittel - Probenahme- und Untersuchungsverfahren - Bestimmung von T-2- und HT-2-Toxinen, Deoxynivalenol und Zearalenon in Einzelfuttermitteln und Mischfuttermitteln mittels LC-MS This European Standard was approved by CEN on 26 September 2016.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
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B-1000 Brussels © 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16877:2016 E SIST EN 16877:2017
Precision data . 15 Annex B (informative)
Examples . 20 B.1 Example 1 . 20 B.1.1 General . 20 B.1.2 LC conditions. 20 B.1.3 MS conditions . 21 SIST EN 16877:2017
Examples of chromatograms according to the settings of the examples in Annex B . 26 Bibliography . 31
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
¶ 10 µg/kg, for DON
¶ 100 µg/kg, and for ZON
¶ 20µg/kg. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 3696:1995, Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) 3 Principle Finely ground and homogeneous test material is suspended in water. After addition of ethyl acetate the sample is agitated. Then sodium sulphate is added to facil
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