EN ISO 14244:2016

SLOVENSKI STANDARD
01-januar-2017
2EURNLL]ROMQLF'RORþHYDQMHYUD]WRSLQLNDOLMHYHJDKLGURNVLGDWRSQLKSURWHLQRY
,62
Oilseed meals - Determination of soluble proteins in potassium hydroxide solution (ISO
14244:2014)
Ölsamenschrote - Bestimmung der in Kaliumhydroxidlösung löslichen Proteine (ISO
14244:2014)
Tourteaux de graines oléagineuses - Détermination de la teneur en protéines solubles en
solution d'hydroxyde de potassium (ISO 14244:2014)
Ta slovenski standard je istoveten z: EN ISO 14244:2016
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 14244
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2016
EUROPÄISCHE NORM
ICS 67.200.20
English Version
Oilseed meals - Determination of soluble proteins in
potassium hydroxide solution (ISO 14244:2014)
Tourteaux de graines oléagineuses - Détermination de Ölsamenschrote - Bestimmung der in
la teneur en protéines solubles en solution Kaliumhydroxidlösung löslichen Proteine (ISO
d'hydroxyde de potassium (ISO 14244:2014) 14244:2014)
This European Standard was approved by CEN on 15 July 2016.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2016 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 14244:2016 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
European foreword
The text of ISO 14244:2014 has been prepared by Technical Committee ISO/TC 34 “Food products” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 14244:2016
by Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-
products - Methods of sampling and analysis” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by February 2017, and conflicting national standards
shall be withdrawn at the latest by February 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 14244:2014 has been approved by CEN as EN ISO 14244:2016 without any modification.

INTERNATIONAL ISO
STANDARD 14244
First edition
2014-02-15
Oilseed meals — Determination
of soluble proteins in potassium
hydroxide solution
Tourteaux de graines oléagineuses — Détermination de la teneur en
protéines solubles en solution d’hydroxyde de potassium
Reference number
ISO 14244:2014(E)
©
ISO 2014
ISO 14244:2014(E)
© ISO 2014
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
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Published in Switzerland
ii © ISO 2014 – All rights reserved

ISO 14244:2014(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Principle . 1
4 Reagents . 1
5 Apparatus . 2
6 Sampling . 2
7 Preparation of test sample . 3
8 Procedure. 3
9 Expression of results . 3
10 Precision . 4
10.1 Interlaboratory test. 4
10.2 Repeatability . 4
10.3 Reproducibility . 4
11 Test report . 4
Annex A (informative) Results of interlaboratory tests. 5
Bibliography
................................................................................................................................................................................................................................ 6
ISO 14244:2014(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the meaning of ISO specific terms and expressions related to conformity
assessment, as well as information about ISO’s adherence to the WTO principles in the Technical Barriers
to Trade (TBT) see the following URL: Foreword - Supplementary information
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 2, Oleaginous
seeds and fruits and oilseed meals.
iv © ISO 2014 – All rights reserved

INTERNATIONAL STANDARD ISO 14244:2014(E)
Oilseed meals — Determination of soluble proteins in
potassium hydroxide solution
1 Scope
This International Standard specifies a method for the determination of soluble proteins in potassium
hydroxide solution in soya meals, rapeseed meals and sunflower pellets, which are then assayed using
the Kjeldahl method as specified in ISO 5983-1 and ISO 5983-2.
2 Normative references
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
ISO 565, Test sieves — Metal wire cloth, perforated metal plate and electroformed sheet — Nominal sizes
of openings
ISO 5500, Oilseed residues — Sampling
ISO 5502, Oilseed residues — Preparation of test samples
ISO 5983-1, Animal feeding stuffs — Determination of nitrogen content and calculation of crude protein
content — Part 1: Kjeldahl method
ISO 5983-2, Animal feeding stuffs — Determination of nitrogen content and calculation of crude protein
content — Part 2: Block digestion and steam distillation method
3 Principle
The sample is dispersed in a solution of potassium hydroxide of approximately 12,5 pH, stirred and
centrifuged. Then, the nitrogen content of the clarified liquid is determined by the Kjeldahl method for
crude protein and compared with the value of crude protein of the original sample.
NOTE The Kjeldahl method is described in ISO 5983-1 and ISO 5983-2.
4 Reagents
WARNING 1 The tests, according to this International Standard, involve risks for persons and the possibility
of releasing substances which might cause damage to the environment. For this reason, appropriate measures
shall be taken to prevent risk, protect personnel, and avoid the release of the substances involved.
WARNING 2 Attention shall be paid to preserving the environment in all phases of this activity. For further
information, it is recommended to make reference to ASTM D4447, which describes the classification of the kind
of residues and pretreatment methods for their recovery or disposal.
Use only reagents of recognized analytical grade.
4.1 Potassium hydroxide.
4.2 Potassium hydroxide solution, c(KOH) = 0,036 mol/l.
Preparation: Dissolve 2,4 g of potassium hydroxide (mass fraction w = 85 g/100 g) in 1 000 ml of distilled
water.
ISO 14244:2014(E)
4.3 n-hexane or hexane mixed isomers or petroleum ether.
5 Apparatus
Usual laboratory apparatus and, in particular, the following.
5.1 Sieve, 500 μm for sunflower pellets and 250 μm for soya and rapeseed meals (as specified in
ISO 565).
5.2 Analytical balance, capable of weighting to the nearest 0,001 g.
5.3 Stirrer’s vessels, of 150 ml capa
...