EN ISO 13366-1:2008

SLOVENSKI STANDARD
01-maj-2008
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SIST EN ISO 13366-1:1998
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5HIHUHQþQDPHWRGD,62
Milk - Enumeration of somatic cells - Part 1: Microscopic method (Reference method)
(ISO 13366-1:2008)
Milch - Zählung somatischer Zellen - Teil 1: Mikroskopisches Verfahren
(Referenzverfahren) (ISO 13366-1:2008)
Lait - Dénombrement des cellules somatiques - Partie 1: Méthode au microscope
(Méthode de référence) (ISO 13366-1:2008)
Ta slovenski standard je istoveten z: EN ISO 13366-1:2008
ICS:
67.100.10
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 13366-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2008
ICS 67.100.10 Supersedes EN ISO 13366-1:1997
English Version
Milk - Enumeration of somatic cells - Part 1: Microscopic method
(Reference method) (ISO 13366-1:2008)
Lait - Dénombrement des cellules somatiques - Partie 1: Milch - Zählung somatischer Zellen - Teil 1:
Méthode au microscope (Méthode de référence) (ISO Mikroskopisches Verfahren (Referenzverfahren) (ISO
13366-1:2008) 13366-1:2008)
This European Standard was approved by CEN on 3 February 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 13366-1:2008: E
worldwide for CEN national Members.

Contents Page
Foreword.3

Foreword
This document (EN ISO 13366-1:2008) has been prepared by Technical Committee ISO/TC 34 "Agricultural
food products" in collaboration with Technical Committee CEN/TC 302 "Milk and milk products - Methods of
sampling and analysis", the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2008, and conflicting national standards shall be withdrawn at
the latest by August 2008.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 13366-1:1997.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 13366-1:2008 has been approved by CEN as a EN ISO 13366-1:2008 without any
modification.
INTERNATIONAL ISO
STANDARD 13366-1
IDF
148-1
Second edition
2008-02-15
Milk — Enumeration of somatic cells —
Part 1:
Microscopic method (Reference method)
Lait — Dénombrement des cellules somatiques —
Partie 1: Méthode au microscope (Méthode de référence)

Reference numbers
ISO 13366-1:2008(E)
IDF 148-1:2008(E)
©
ISO and IDF 2008
ISO 13366-1:2008(E)
IDF 148-1:2008(E)
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Published in Switzerland
ii © ISO and IDF 2008 – All rights reserved

ISO 13366-1:2008(E)
IDF 148-1:2008(E)
Contents Page
Foreword. iv
Foreword. v
1 Scope .1
2 Terms and definitions .1
3 Principle.1
4 Reagents.1
4.1 Dye solutions .1
4.2 Phosphate Buffer Solution (PBS).3
5 Apparatus .4
6 Sampling.4
7 Preparation of test sample.4
7.1 Storage.4
7.2 Preparation .5
8 Procedure .5
8.1 Preparation of the smear and staining .5
8.2 Determination.6
9 Calculation and expression of results.10
9.1 Rectangular shape counting in successive fields .10
9.2 Rectangular shape counting in bands .11
9.3 Circular shape counting in successive fields.11
9.4 Expression of results .12
10 Precision.12
10.1 Repeatability.12
10.2 Reproducibility.12
11 Test report .13
Annex A (informative) Collaborative trial.14
Annex B (informative) Staining for goats' milk .15
Annex C (informative) Poisson distribution .16
Bibliography .17
ISO 13366-1:2008(E)
IDF 148-1:2008(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has
been established has the right to be represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 13366-1|IDF 148-1 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee
SC 5, Milk and milk products, and the International Dairy Federation (IDF). It is being published jointly by ISO
and IDF.
This second edition of ISO 13366-1|IDF 148-1 cancels and replaces the first edition (ISO 13366-1:1997), of
which it constitutes a technical revision.
ISO 13366 consists of the following parts, under the general title Milk — Enumeration of somatic cells:
⎯ Part 1: Microscopic method (Reference method)
⎯ Part 2: Guidance on the operation of fluoro-opto-electronic counters

iv © ISO and IDF 2008 – All rights reserved

ISO 13366-1:2008(E)
IDF 148-1:2008(E)
Foreword
IDF (the International Dairy Federation) is a non-profit organization representing the dairy sector worldwide.
IDF membership comprises National Committees in every member country as well as regional dairy
associations having signed a formal agreement on cooperation with IDF. All members of IDF have the right to
be represented at the IDF Standing Committees carrying out the technical work. IDF collaborates with ISO in
the development of standard methods of analysis and sampling for milk and milk products.
Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the
National Committees for voting. Publication as an International Standard requires approval by at least 50 % of
the IDF National Committees casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. IDF shall not be held responsible for identifying any or all such patent rights.
ISO 13366-1|IDF 148-1 was prepared by the International Dairy Federation (IDF) and Technical Committee
ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products. It is being published jointly by IDF
and ISO.
All work was carried out by the Joint ISO-IDF Action Team Automated methods of the Standing Committee on
Quality assurance, statistics of analytical data and sampling under the aegis of its project leaders, Mrs. S.
Orlandini (IT) and Mr. H.J.C.M. van den Bijgaart (NL).
This edition of ISO 13366-1|IDF 148-1 cancels and replaces IDF 148A:1995.
ISO 13366 consists of the following parts, under the general title Milk — Enumeration of so
...