ENV 807:2001

SLOVENSKI STANDARD
01-januar-2004
=DãþLWQDVUHGVWYD]DOHV±8JRWDYOMDQMHXþLQNRYLWRVWL]DãþLWQHJDVUHGVWYDSURWL
JOLYDPUD]NURMHYDONDPOHVD
Wood preservatives - Determination of the effectiveness against soft rotting micro-fungi
and other soil inhabiting micro-organisms
Holzschutzmittel - Prüfverfahren für die Bestimmung der Grenze der Wirksamkeit gegen
Moderfäule und andere erdbewohnende Mikroorganismen
Produits de préservation du bois - Détermination de l'efficacité vis-a-vis des micro-
organismes de pourriture molle et d'autres micro-organismes du sol
Ta slovenski standard je istoveten z: ENV 807:2001
ICS:
71.100.50 .HPLNDOLMH]D]DãþLWROHVD Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN PRESTANDARD
ENV 807
PRÉNORME EUROPÉENNE
EUROPÄISCHE VORNORM
May 2001
ICS 71.100.50 Supersedes ENV 807:1993
English version
Wood preservatives - Determination of the effectiveness against
soft rotting micro-fungi and other soil inhabiting micro-organisms
Produits de préservation du bois - Détermination de Holzschutzmittel - Prüfverfahren für die Bestimmung der
l'efficacité vis-à-vis des micro-organismes de pourriture Grenze der Wirksamkeit gegen Moderfäule und andere
molle et d'autres micro-organismes du sol erdbewohnende Mikroorganismen
This European Prestandard (ENV) was approved by CEN on 1 March 2001 as a prospective standard for provisional application.
The period of validity of this ENV is limited initially to three years. After two years the members of CEN will be requested to submit their
comments, particularly on the question whether the ENV can be converted into a European Standard.
CEN members are required to announce the existence of this ENV in the same way as for an EN and to make the ENV available promptly
at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the ENV) until the final
decision about the possible conversion of the ENV into an EN is reached.
CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,
Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2001 CEN All rights of exploitation in any form and by any means reserved Ref. No. ENV 807:2001 E
worldwide for CEN national Members.

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Contents
page
Foreword.3
Introduction .4
1 Scope .5
2 Normative references .5
3 Terms and definitions.5
4 Principle.5
5 Test materials.5
6 Sample of the preservative .7
7 Test specimens .7
8 Procedures .9
9 Validity of test.13
10 Expression of results .13
11 Test report .14
Annex A (informative) Optional screening test .16
Annex B (informative) Determination of soil water holding capacity .26
Annex C (informative) Rapid soil virulence test.29
Annex D (informative) Experimental set up of the test containers .30
Annex E (informative) Calculation of the nominal effective retention.31
Annex F (informative) Example of a test report .35
Bibliography .41

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Foreword
This European Prestandard has been prepared by Technical Committee CEN/TC 38, "Durability of wood and
derived materials", the secretariat of which is held by AFNOR.
This European Prestandard supersedes ENV 807:1993.
The annexes A, B, C, D, E and F are informative.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to announce this European Prestandard: Austria, Belgium, Czech Republic, Denmark, Finland,
France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden,
Switzerland and the United Kingdom.

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Introduction
This European Prestandard specifies a laboratory method of test which gives a basis for assessing the
effectiveness of a wood preservative against micro-fungi (ascomycetes and fungi imperfecti) which cause soft rot of
wood in service. The infection source is the natural micro-flora of the soil which may also contain other
micro-organisms, such as bacteria and other fungi, such as moulds and basidiomycetes. This laboratory method
provides one criterion by which the value of a wood preservative product can be assessed. This information has to
be supplemented by data from other relevant tests and from practical experience.

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1 Scope
This European Prestandard specifies a method of test for determining the toxic effectiveness of a wood
preservative, applied to wood by full impregnation, against the micro-fungi which cause soft rot of wood.
The method is applicable to testing of formulated products or of their active ingredients.
NOTE A method suitable for undertaking screening tests of potential active ingredients is given in annex A.
2 Normative references
This European Prestandard incorporates by dated or undated reference, provisions from other publications. These
normative references are cited at the appropriate places in the text and the publications are listed hereafter. For
dated references, subsequent amendments to or revisions of any of these publications apply to this European
Prestandard only when incorporated in it by amendment or revision. For undated references the latest edition of the
publication referred to applies (including amendments).
EN 84, Wood preservatives — Accelerated ageing of treated wood prior to biological testing — Leaching procedure
EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this European Prestandard, the following terms and definitions apply:
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average characteristics of the total
volume being sampled
3.2
supplier
sponsor of the test
4 Principle
A number of small test specimens (as small stakes) are impregnated with the preservative under test at a minimum
of three concentrations ranged about the retention expected to provide protection throughout the test period. The
test specimens are exposed to leaching according to EN 84. The specimens are partly buried vertically in a
microbially active soil. Sets of test specimens are assessed after 8, 16, 24 and 32 weeks of exposure. The
performance of the test preservative is evaluated by comparison with the performance of a reference preservative.
5 Test materials
5.1 Biological materials
5.1.1 Soil
1)
Natural top soil or a fertile loam-based horticultural soil of pH 6 to pH 8 and not containing added agro-chemicals.
It shall have a waterholding capacity (WHC) of between 25 % (m/m) and 60 % (m/m).
NOTE 1 A suitable method for determining WHC is described in annex B.

1)
A horticultural soil of the John Innes No.2 type and with the following composition has been found to be suitable; seven
parts by volume loam, three parts by volume sphagnum peat, two parts by volume sharp sand plus 0,6 g chalk and 6,0 g slow
release fertilizer per litre of soil mixture. If the WHC is too high, it can be lowered by modifying the soil with the addition of sand.

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If a natural soil is used, it shall have the turf or top 50 mm removed and shall not be taken from a depth below
200 mm from the original surface. It shall be passed through a sieve of nominal aperture size 12,5 mm. If it is
necessary to store the soil prior to use, it shall be stored in closed moisture-proof containers. Before use,
thoroughly mix the sample of soil.
NOTE 2 The soil should only be collected in a moist condition.
If a horticultural soil is used which is sterilized during its preparation, then 20 % (m/m) of a natural soil, prepared as
above, shall be added and the soils thoroughly mixed prior to the start of the test.
The soil shall be used only once.
NOTE 3 If assurance of the virulence of the soil is required, the test procedure using cotton cloth described in annex C, or a
similar standardized procedure, may be used.
5.2 Products and reagents
5.2.1 Solvents and diluents
Water to grade 3 of EN ISO 3696 and, if appropriate, volatile organic liquids leaving in the wood no residue which
would have a toxic effect on the soil inhabiting micro-organisms at the end of the post-treatment conditioning
period.
NOTE Information on appropriate solvents and diluents should be provided by the supplier.
5.2.2 Reference preservative
A copper/chromium preservative with a composition equivalent to the following :
.
CuSO 5H 0 50,0 % (m/m)
4 2
K Cr 0 48,0 % (m/m)
2 2 7
CrO 2,0 % (m/m)
The preservative shall be prepared from ingredients of at least 95 % (m/m) purity.
5.2.3 Xylene
(C H (CH ) ) mixed isomers, technical grade.
6 4 3 2
5.3 Apparatus
5.3.1 Conditioning chamber, well ventilated and maintained at (20 ± 2) °C and (65 ± 5) % r.h.
5.3.2 Ventilated drying oven, capable of being maintained at (103 ± 2) °C.
5.3.3 Desiccators, with efficient desiccant (silica gel for example).
5.3.4 Treatment vessels, of a material that does not react with either the preservative or solvents or diluents, for
example of glass for organic products and plastics materials for salts containing fluorine.
5.3.5 Weights, of a material that does not react with the preservative solutions under test, to provide ballast for
the test specimens.
5.3.6 Plastics mesh, of a material that does not react with the preservative solutions under test, for retaining test
specimens during impregnation.
5.3.7 Vacuum vessels, fitted with stopcocks.
5.3.8 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 0,7 kPa.

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5.3.9 Drying vessels, provided with a cover and containing supports which will give a minimum of contact with
the treated test specimens which are to be placed on them. The vessels and supports shall be of a material that
does not react with the test solvent or test preservative, for example glass for organic products or of plastic material
for salts containing fluorine.
5.3.10 Culture chamber (incubator or room), dark and maintained at (27 ± 2) °C and (70 ± 5) % r.h.
5.3.11 Vacuum filtration apparatus,
comprising vacuum flask, 146 mm diameter Buchner funnel and fitting
coarse grade filter papers.
5.3.12 Test containers, made of material which does not have a toxic effect on the soil inhabiting micro-
organisms and provided with a ventilated lid. The depth shall be at least 150 mm, so as to provide at least 30 mm
below the test specimens when inserted in the soil to a depth of 80 mm and adequate clearance above the top of
the protruding parts of the test specimens.
NOTE The exact dimensions are not critical but they determine the number of test specimens in each vessel (which should
not be less than 10). An example of a suitable test container is described in annex D.
5.3.13 Safety equipment and protective clothing,
appropriate for the test product, test solvent and reference
preservative, to ensure the safety of the operator.
5.3.14 Ordinary laboratory equipment, including a balance accurate to 0,001 g.
6 Sample of the preservative
The sample of preservative shall be representative of the product to be tested. Samples shall be stored and
handled in accordance with any written recommendations from the supplier.
NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The following species shall be used for the test :
Scots pine (Pinus sylvestris Linnaeus
...