EN 1390:2006

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Wood preservatives - Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae - Laboratory methodProduits de préservation du bois - Détermination de l'action curative contre les larves d'Hylotrupes bajulus (Linnaeus) - Méthode de laboratoireHolzschutzmittel - Bestimmung der bekämpfeden Wirkung gegenüber Larven von Hylotrupes bajulus (Linnaeus) - LaboratoriumsverfharenTa slovenski standard je istoveten z:EN 1390:2006SIST EN 1390:2006en71.100.50Wood-protecting chemicalsICS:SIST-TS ENV 1390:20041DGRPHãþDSLOVENSKI
STANDARDSIST EN 1390:200601-december-2006

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 1390June 2006ICS 71.100.50Supersedes ENV 1390:1994
English VersionWood preservatives - Determination of the eradicant actionagainst Hylotrupes bajulus (Linnaeus) larvae - LaboratorymethodProduits de préservation du bois - Détermination de l'actioncurative contre les larves d'Hylotrupes bajulus (Linnaeus) -Méthode de laboratoireHolzschutzmittel - Bestimmung der bekämpfeden Wirkunggegenüber Larven von Hylotrupes bajulus (Linnaeus) -LaboratoriumsverfharenThis European Standard was approved by CEN on 24 May 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2006 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 1390:2006: E

Example of a test report.14 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus.16 Annex C (informative) Environmental, health and safety precautions within chemical/biological laboratory.19 Bibliography.20

or organic water-dispersible formulations, as supplied or as prepared in the laboratory by dilution of concentrates, or water-soluble products, for example, salts. NOTE An ageing procedure cannot be combined with this method. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 3696,Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) ISO 835-1:1981, Laboratory glassware - Graduated pipettes - Part 1: General requirements 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample with physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled [EN 1001-2:2005, 4.71] 3.2
supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) [Adapted from EN 1001-2:2005, 4.83] 4 Principle Insertion of larvae of Hylotrupes bajulus into test specimens. After a period of time to allow the larvae to establish themselves in the test specimens, treatment of these test specimens by brushing or pipetting of the test preservative product. After the time necessary for the preservative to act effectively, assessment of the mortality of the larvae compared with that of larvae in untreated control test specimens.

5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) larvae 5.1.2 Source of larvae. The larvae shall preferably be obtained from cultures reared according to the method described in Annex B.
NOTE Larvae can also be taken from naturally infested wood, in which case they should be transferred into sapwood of pine and stored for at least four weeks under the rearing conditions specified in Annex B. Do not use the larvae in the test if they have not fed normally during this storage period. 5.1.3
Provision of larvae Carefully split or crumble infested blocks to extract larvae. Keep the larvae separate from one another in glass receptacles for two or three days in the culturing chamber (5.3.1) to check they are healthy. 5.1.4 Choice of larvae Use only healthy larvae in the test. NOTE 1 A healthy larva can be recognized by ivory-white colour, its firm consistency and rounded appearance, and by the absence of wounds or bites, which show up as dark marks. Healthy larvae react to the touch by vigorous movement and attempts to bite. Reject any larvae, which are shrunken or aged which have recently moulted, or which are in a pre-pupal stage. Weigh each larva and place it in a glass receptacle marking the receptacle with the weight of the larva. Make up two groups with the weight ranges: - 51 mg to 100 mg and -101 mg to 150 mg. NOTE 2
Larvae with a mass larger than 150 mg in mass are unsuitable as they can pupate during the course of the test. 5.2
Products and reagents 5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which water is the continuous phase. NOTE
Paraffin wax with a setting point of 52 °C to 54 °C has been found suitable. 5.2.2 Gelatine, for sealing the relevant surfaces of specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION — Do not use benzene or other solvents which pose a health risk.

Culturing chamber, with air circulation, controlled at (28 ± 2) °C and at a relative humidity of
(70 ± 5) %. 5.3.2
Ventilated fume cupboard, in which the specimens are treated with an input air temperature of (20 ± 5) °C and a maximum air speed, measured at the input opening with the sash in the approximate operating position, of 0,5 m/s. CAUTION - It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.3 Testing chamber, ventilated and controlled at (21 ± 2) °C and at a relative humidity of (75 ± 5) %. 5.3.4
Drill and twist drills, with 3 mm, 4 mm and 5 mm diameters. 5.3.5 Pipettes as specified in ISO 835-1:1981, Class B - graduated pipette with no waiting time, with a capacity 5 ml and an accuracy of ± 0,05 ml. 5.3.6 Safety equipment and protective clothing, appropriate for the test product and the test, to ensure the safety of the operator. 5.3.7 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 1 mg. 5.3.8 Rectangular cover with sides, constructed either of glass, plastics, plywood and of a height not less than 200 mm and with an open face of sufficient size to cover all the treated specimens from a single test.
5.3.9 X-ray apparatus (optional) with tungsten target and beryllium window, with voltage and current continuously variable in the ranges: -voltage: 10 kV to 50 kV; -current: 0 mA to 15 mA. 5.3.10 Protective gloves 6
Sampling The sample of preservative shall represent the product to be tested. Samples shall be stored and handled in accordance with any written recommendations from the supplier. NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used. 7 Test specimens 7.1 Species of wood The reference species is Scots pine (Pinus sylvestris Linnaeus)1). NOTE Additional tests may be carried out using other species but, if so, this should be stated in the test report.
1) In southern European countries the species of pine most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this standard has been demonstrated in all aspects (development of larvae, resistance to impregnation, etc.).

Wood that has been kiln dried at temperatures below 60 °C may be used. The wood shall be exclusively sapwood containing little resin and having between 2,5 annual rings per 10 mm and eight annual rings per 10 mm. The proportion of latewood in the annual rings shall not exceed 30 % of the whole. NOTE 2 It is recommended to use test specimens of a similar growth rate within a single test. 7.3 Provision of test specimens Prepare planed strips having a cross-section of (100 ± 2) mm x (25 ± 2) mm removing a minimum of 2 mm from any surface exposed during drying. The longitudinal faces shall be parallel to the direction of the grain. The annual rings shall be parallel to the broad faces (contact angle of less than 35 °), (see Figure 1). Make transverse cuts neatly to give sharp edges and a fine-sawn finish to the end-grain surfaces, to make test specimens (150 ± 2) mm long. The test specimens shall originate from a minimum of three trees or shall be taken at random from a stock originally of more than 100 test specimens. 7.4 Dimensions of test specimens The test specimen at (12 ± 2) % (m/m) moisture content shall be (150 ± 2) mm x (100 ± 2) mm x (25 ± 1) mm. NOTE
A moisture meter of the two-pronged electrical conductivity type is suitable for assessing moisture content. Mark each test specimen so that it can be identified throughout the test. 7.5 Number of test specimens 7.5.1 For fast and slow acting wood preservatives, test duration 12 weeks or 24 weeks Use: a) for each wood preservative, each concentration and each method of treatment -10 treated test specimens; b) for a single test of each wood preservative - two untreated control test specimens. 7.5.2 For deferred acting wood preservatives, test duration 52 weeks Use: a) for each wood preservative, each concentration and each method of treatment- 20 treated
test specimens; b) for a single test of each wood preservative -four untreated control test specimens.
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