EN ISO 10519:2000

SLOVENSKI STANDARD
01-februar-2001
6HPHROMQHUHSLFH'RORþHYDQMHNORURILOD6SHNWURPHWULMVNDPHWRGD,62

Rapeseed - Determination of chlorophyll content - Spectrometric method (ISO
10519:1997)
Rapssamen - Bestimmung des Chlorophyllgehaltes -Spektrometrisches Verfahren (ISO
10519:1997)
Graines de colza - Détermination de la teneur en chlorophylle -Méthode spectrométrique
(ISO 10519:1997)
Ta slovenski standard je istoveten z: EN ISO 10519:2000
ICS:
67.200.20 Oljnice Oilseeds
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

INTERNATIONAL ISO
STANDARD 10519
Second edition
1997-11-01
Rapeseed — Determination of chlorophyll
content — Spectrometric method
Graines de colza — Détermination de la teneur en chlorophylle — Méthode
spectrométrique
Reference number
A
ISO 10519:1997(E)
ISO 10519:1997(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are circulated to the member bodies for
voting. Publication as an International Standard requires approval by at least 75 % of the member bodies
casting a vote.
International Standard ISO 10519 was prepared by Technical Committee ISO/TC 34, Agricultural food
products, Subcommittee SC 2, Oleaginous seeds and fruits.
This second edition cancels and replaces the first edition (ISO 10519:1992), which has been technically
revised.
Annexes A and B of this International Standard are for information only.
©  ISO 1997
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic
or mechanical, including photocopying and microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case Postale 56 • CH-1211 Genève 20 • Switzerland
Internet central@iso.ch
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Printed in Switzerland
ii
INTERNATIONAL STANDARD © ISO ISO 10519:1997(E)

Rapeseed — Determination of chlorophyll content —
Spectrometric method
1 Scope
This International Standard specifies a spectrometric method for the determination of the
chlorophyll content of rapeseed. It is not applicable to the determination of chlorophyll in oils.
2 Normative references
The following standards contain provisions which, through reference in this test, constitute
provisions of this International Standard. At the time of the publication, the editions indicated
were valid. All standards are subject to revision, and the parties to agreements based on this
International Standard are encouraged to investigate the possibility of applying the most recent
editions of the standards indicated below. Members of IEC and ISO maintain registers of
currently valid International Standards.
ISO 648:1977, Laboratory glassware - One-mark pipettes.
ISO 664:1990, Oilseeds - Reduction of laboratory sample to test sample.
ISO 665:1977, Oilseeds - Determination of moisture and volatile matter content.
3 Definition
For the purposes of this International Standard, the following definition applies.
3.1
chlorophyll content
mass fraction of substances in the sample contributing to the absorption band at a wavelength
near 665 nm, as determined under the operating conditions specified in this International
Standard and measured as chlorophyll A
NOTE —  The chlorophyll content is expressed in milligrams per kilogram.
4 Principle
Extraction of a test portion in a suitable apparatus with a specified extraction solvent.
Spectrometric determination of the chlorophyll content of the extracted solution
5 Reagent
Use only reagents of recognized analytical grade unless otherwise stated.
ISO 10519:1997(E) © ISO
5.1 Extraction solvent
Transfer to a 500 ml beaker 100 ml of anhydrous ethanol. Add to the contents of the
beaker 300 ml of anhydrous iso-octane (2,2,5-trimethylpentane) or anhydrous technical n-hep-
tane or anhydrous petroleum ether (essentially composed of C hydrocarbons, with a boiling
range between 90 °C and 100 °C).
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
6.1 Analytical balance, capable of weighing to the nearest 0,001 g.
6.2 Mechanical grinder, blade type, or coffee mill or equivalent.
(see figure 1), comprising stainless-steel tubes of approx-
6.3 Mechanical microgrinder
imately 50 ml volume which can be securely stoppered, stainless-steel ball-bearings (∅ 16 mm),
and an apparatus to shake the securely stoppered tubes horizontally at a frequency of
–1 1)
240 min , with a horizontal displacement of 3,5 cm, or a Dangoumau ball mill .
Dimensions in millimetres
Figure 1 — Mechanical microgrinder
6.4 Filter paper, medium speed, V-folded.
6.5 Spectrometer (preferably with wavelength scanning), suitable for carrying out absorbance
measurements at wavelengths between 600 nm and 700 nm, with a spectral bandwidth of 2 nm.
6.6 Optical cells, having a path length of at least 1 cm.
6.7 Pipettes, of 30 ml capacity, complying with the requirements of ISO 648, class A, or a
repetitive dispenser capable of dispensing 30 ml with an error of less than 1 %.
6.8 Culture tubes, of 20 ml capacity, provided with stoppers.

1) Dangoumau ball mill is an example of a suitable apparatus available commercially. This information is given for the
convenience of users of this International Standard and does not constitute an endorsement by ISO of this apparatus.
SIST EN ISO 1051
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