FprEN 17700-3

SLOVENSKI STANDARD
oSIST prEN 17700-3:2023
01-maj-2023
Rastlinski biostimulanti - Navedbe - 3. del: Toleranca na abiotični stres pri
rastlinah zaradi uporabe biostimulanta
Plant biostimulants - Claims - Part 3: Tolerance to abiotic stress resulting from the use of
a plant biostimulan
Pflanzen-Biostimulanzien - Auslobungen - Teil 3: Toleranz gegenüber abiotischem
Stress infolge der Verwendung eines Pflanzen-Biostimulans
Biostimulants des végétaux - Allégations - Partie 3 : Tolérance au stress abiotique
résultant de l'utilisation d'un biostimulant des végétaux
Ta slovenski standard je istoveten z: prEN 17700-3
ICS:
65.080 Gnojila Fertilizers
oSIST prEN 17700-3:2023 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

oSIST prEN 17700-3:2023
oSIST prEN 17700-3:2023
DRAFT
EUROPEAN STANDARD
prEN 17700-3
NORME EUROPÉENNE
EUROPÄISCHE NORM
December 2023
ICS 65.080 Will supersede CEN/TS 17700-3:2022
English Version
Plant biostimulants - Claims - Part 3: Tolerance to abiotic
stress resulting from the use of a plant biostimulant
Biostimulants des végétaux - Allégations - Partie 3 : Pflanzen-Biostimulanzien - Auslobungen - Teil 3:
Tolérance au stress abiotique résultant de l'utilisation Toleranz gegenüber abiotischem Stress infolge der
d'un biostimulant des végétaux Verwendung eines Pflanzen-Biostimulans
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 455.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17700-3:2023 E
worldwide for CEN national Members.

oSIST prEN 17700-3:2023
prEN 17700-3:2023 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Terminology of the claim . 7
5 Justification of plant biostimulant claims regarding abiotic stress tolerance . 7
5.1 Introduction . 7
5.2 Trial design with negative control . 8
5.3 Trial design without negative control . 8
6 Agronomic markers to validate the claim . 9
7 Specifications for the performance of the trials .11
Annex A (informative) Rationale for trial designs .12
Annex ZA (informative) Relationship of this European Standard and the essential
requirements of Regulation (EU) 2019/1009 making available on the market of EU
fertilizing products aimed to be covered .13
oSIST prEN 17700-3:2023
prEN 17700-3:2023 (E)
European foreword
This document (prEN 17700-3:2023) has been prepared by Technical Committee CEN/TC 455 “Plant
Biostimulants”, the secretariat of which is held by AFNOR.
This document is currently submitted to the CEN Enquiry.
This document will supersede CEN/TS 17700-3:2022.
prEN 17700-3:2023 includes the following significant technical changes with respect to
CEN/TS 17700-3:2022:
— Addition of some definitions;
— Addition of a flowchart diagram explaining the rationale for trial designs in Annex A.
This document has been prepared under a Standardization Request given to CEN by the European
Commission and the European Free Trade Association, and supports essential requirements of EU
Directive(s) / Regulation(s).
For relationship with EU Directive(s) / Regulation(s), see informative Annex ZA, which is an integral part
of this document.
The EN 17700 series, Plant biostimulants — Claims, consists of the following parts:
— Part 1:General principles;
— Part 2: Nutrient use efficiency resulting from the use of a plant biostimulant;
— Part 3: Tolerance to abiotic stress resulting from the use of a plant biostimulant;
— Part 4: Determination of quality traits, resulting from the use of a plant biostimulant;
— Part 5: Determination of availability of confined nutrients in the soil or rhizosphere.
oSIST prEN 17700-3:2023
prEN 17700-3:2023 (E)
Introduction
This document has been developed to provide guidance for a consistent approach to justify the claims
associated with the use of plant biostimulants in agriculture.
The definition of plant biostimulants to be used in the regulation on fertilizing materials is claims-based.
For this reason, demonstrating that a product is indeed a bona fide plant biostimulant depends on a
demonstration of its effect.
The placing of a plant biostimulant on the market should not be considered to guarantee effectiveness
under all conditions, as many factors may influence the performance of a plant biostimulant in the field.
Plant biostimulants used in agriculture can be applied in multiple ways: on soil, on plant, as seed
treatment, etc. This document is applicable to all application types of plant biostimulants in agriculture.

oSIST prEN 17700-3:2023
prEN 17700-3:2023 (E)
1 Scope
This document provides guidance for justifying abiotic stress tolerance claim of plant biostimulants used
in agriculture.
This document is aimed primarily at manufacturers, laboratories, companies which will put the products
on the market, notifying authorities, notified bodies, and market surveillance authorities.
To be in compliance with this standard, it is important also to follow the Recommendations and Quality
Criteria described in the Standard of General Principles EN 17700-1:— .
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 17700-1:— , Plant biostimulants — Claims — Part 1: General principles
EN 17724:— , Plant biostimulants — Terminology
3 Terms and definitions
1 2
For the purposes of this document, the terms and definitions given in EN 17700-1:— , EN 17724:— , and
the following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp/
— IEC Electropedia: available at https://www.electropedia.org/
3.1
tolerance to abiotic stress
ability to endure abiotic stress
3.2
agronomic marker
measurable plant trait used to validate the claim
EXAMPLE Refer to Clause 6 of this document.
3.3
stress marker
physiological, biochemical and molecular traits associated with a plant response to a specific stress
EXAMPLE Heat shock proteins, electrolyte leakage for thermal stress, chlorophyll fluorescence, lipid
peroxidation for light stress, electrolyte leakage, lipid peroxidation for mechanical stress, relative water content,
electrolyte leakage for water stress.

Under preparation
Under preparation
oSIST prEN 17700-3:2023
prEN 17700-3:2023 (E)
3.4
abiotic stress
negative impact of non-living factors on the plant in a specific crop environment
Note 1 to entry: Crop tolerance to abiotic stress is addressed to one or more (multiple or combined) of the following
stress categories:
1) thermal stress,
2) light stress,
3) mechanical stress,
4) water stress,
5) chemical stress.
3.5
thermal stress
negative impact of temperature (supra-optimal and sub-optimal temperature) on the plant in a specific
crop environment
EXAMPLE heat stress or cold stress such as chilling and freezing stress
3.6
light stress
negative impact of light intensity and/or spectrum on the plant in a specific crop environment
EXAMPLE high irradiance or low irradiance, UV radiation
3.7
mechanical stress
negative impact of a mechanical force on the plant or the root zone in a specific crop environment
EXAMPLE wind, hail, agricultural operations
3.8
water stress
negative impact of water or high solutes concentrations or excessive transpiration on the plant in a
specific crop environment
EXAMPLE drought, high air vapour pressure deficit, flooding
3.9
chemical stress
negative impact of chemicals (supra-optimal or sub-optimal chemical compounds or presence) on the
plant in a specific crop environment
EXAMPLE salt stress, mineral toxicity induced by heavy metals or excessive application of mineral nutrients,
adverse pH conditions, ozone stress, phytotoxic effects of xenobiotics
oSIST prEN 17700-3:2023
prEN 17700-3:2023 (E)
3.10
xenobiotic
chemical substance found within an organism that is not naturally produced or expected to be present
within the organism
EXAMPLE heavy metals, ozone
4 Terminology of the claim
The type of claim(s) included in the European Standard can be addressed to one or more of the above
defined abiotic stress(es) (see 3.5 to 3.7).
For instance, a plant biostimulant can improve plant tolerance to a single abiotic stress factor such as
heat stress or to multiple abiotic stress factors such as heat stress and salt stress in one or more target
crop(s).
Label shall clearly indicate that the plant biostimulant is addressed to improve tolerance to abiotic stress
with a clear indication of the type of abiotic stress(es) and the crop(s) on which the effect is demonstrated
according to EN 17700-1:— .
EXAMPLE “improves tolerance to drought stress on woody perennials”, “improves tolerance to cold stress on
tomato”
5 Justification of plant biostimulant claims regarding abiotic stress tolerance
5.1 Introduction
In open field conditions it can be difficult to fully control specific abiotic stress factors due to their
inherent variability and due to the fact, that, often several abiotic stresses may be present at the same
time (combinational stresses). Therefore, to fully validate an abiotic stress tolerance claim with both a
negative (untreated and unstressed) control and a positive (untreated and stressed) control, trial should
take place under controlled conditions (e.g. growth chamber, greenhouse).
By contrasting and comparing negative and positive control treatments, a researcher can verify the stress
experienced by the plant. Or in other words, how much damage/growth depression was caused by the
stress on the plant without any biostimulant application.
In some situations, a negative control might not be easily implemented in the same growing environment.
In this case, the emphasis is placed more on detecting the specific stress by assessing specific markers
within the plant response to both the environment (growing conditions) and the plant biostimulant
application.
In order to justify the claim, these stress markers shall be of agronomic significance (specific to the stress
being examined e.g. heat shock proteins created by heat stress). Their relevance may be proved through
referencing previous peer reviewed literature that correlated these markers to the stress being
examined. Often this stress marker (be it physiological, biochemical or genetic) will cause a plant
response (change in phenotype) which will either be promoted or reduced through the use of
biostimulants.
Therefore, assessing the stress marker in both the positive control (untreated and stressed) versus the
treated (product to be tested) group, will allow a researcher to assess the presence of the stress and how
much of a change was caused in the plant response by applying the product, allowing trials without a
negative control to validate abiotic stress tolerance.
A flowchart diagram explaining the rationale for trial designs is given in Annex A (informative).
oSIST prEN 17700
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