EN 16421:2014

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Vpliv materialov na pitno vodo - Spodbujanje mikrobiološke rastiEinfluss von Materialien auf Wasser für den menschlichen Gebrauch - Förderung des mikrobiellen WachstumsInfluence des matériaux sur l'eau destinée à la consommation humaine - Stimulation de la croissance microbienne (SCM)Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)67.250Materiali in predmeti v stiku z živiliMaterials and articles in contact with foodstuffs13.060.20Pitna vodaDrinking water07.100.20Mikrobiologija vodeMicrobiology of waterICS:Ta slovenski standard je istoveten z:EN 16421:2014SIST EN 16421:2015en,fr01-februar-2015SIST EN 16421:2015SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16421
December 2014 ICS 07.100.20 English Version
Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)
Influence des matériaux sur l'eau destinée à la consommation humaine - Stimulation de la croissance microbienne (SCM)
Einfluss von Materialien auf Wasser für den menschlichen Gebrauch - Förderung des mikrobiellen Wachstums This European Standard was approved by CEN on 25 October 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
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Method 1: Measured by Biomass Production Potential (BPP) measured by ATP – Assessment of the suitability of test water for its use in the BPP test (biological stability and the need for addition of trace elements) . 48 Annex B (normative)
Method 1: Measured by Biomass Production Potential (BPP) measured by ATP – Protocol of ATP analysis . 50 B.1 Introduction . 50 B.2 Apparatus and glassware . 50 B.3 Reagents and auxiliary substances . 50 B.3.1 General . 50 B.3.2 Sterile water . 50 B.3.3 ATP solutions for standard dilutions and calibration curves . 50 B.3.4 Luciferin/luciferase (enzyme) solution . 51 B.3.5 ATP extraction reagent . 52 B.4 Sampling . 52 B.5 Procedure . 52 B.5.1 General . 52 B.5.2 Blank water . 52 B.5.3 Control dilutions . 52 B.5.4 Samples . 52 B.6 Expression of results . 52 B.6.1 Water samples . 52 B.6.2 Material samples . 53 B.7 Test record . 53 SIST EN 16421:2015

Method 1: Measured by Biomass Production Potential (BPP) measured by ATP - ATP and its measurement . 55 Annex D (informative)
Method 1: Measured by Biomass Production Potential (BPP) measured by ATP – Evaluating procedures for removal of attached biomass . 56 D.1 Introduction . 56 D.2 Procedures for removal of Attached Biomass . 56 D.2.1 General . 56 D.2.2 Swabbing . 56 D.2.3 Low energy sonication (LES) . 56 D.2.4 High energy sonication (HES) . 56 D.3 Effectiveness of removal procedure . 57 Annex E (informative) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP . 58 Annex F (informative)
Method 2: Measured by biofilm volume . 59 Annex G (informative)
Method 2: Measured by biofilm volume . 60 Annex H (informative)
Method 3: Measured by mean dissolved oxygen depletion . 63 Bibliography . 65
The results given by each method are not directly comparable. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 901, Chemicals used for treatment of water intended for human consumption — Sodium hypochlorite prEN 1254-1:2007, Copper and copper alloys — Plumbing fittings — Part 1: Fittings with ends for capillary soldering or capillary brazing to copper tubes EN 1484, Water analysis — Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC) EN 10088-1, Stainless steels — Part 1: List of stainless steels EN 14944-1, Influence of cementitious products on water intended for human consumption — Test methods — Part 1: Influence of factory made cementitious products on organoleptic parameters EN ISO 3696:1995, Water for analytical laboratory use — Specification and test methods (ISO 3696:1987) EN ISO 5814, Water quality — Determination of dissolved oxygen — Electrochemical probe method (ISO 5814) EN ISO 7393-2, Water quality — Determination of free chlorine and total chlorine — Part 2: Colorimetric method using N, N-diethyl-1, 4-phenylenediamine, for routine control purposes (ISO 7393-2) EN ISO 9308-1, Water quality — Enumeration of Escherichia coli and coliform bacteria — Part 1: Membrane filtration method for waters with low bacterial background flora (ISO 9308-1) EN ISO 10012, Measurement management systems — Requirements for measurement processes and measuring equipment (ISO 10012) EN ISO 10523, Water quality — Determination of pH (ISO 10523) EN ISO 13385-1, Geometrical product specifications (GPS) — Dimensional measuring equipment — Part 1: Callipers; Design and metrological characteristics (ISO 13385-1) EN ISO 13385-2, Geometrical product specifications (GPS) — Dimensional measuring equipment — Part 2: Calliper depth gauges; Design and metrological characteristics (ISO 13385-2) EN ISO 16266, Water quality — Detection and enumeration of Pseudomonas aeruginosa — Method by membrane filtration (ISO 16266) SIST EN 16421:2015
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