ASTM D3325-90(2013)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation: D3325 − 90 (Reapproved 2013)
Standard Practice for
Preservation of Waterborne Oil Samples
This standard is issued under the fixed designation D3325; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* D3650 Test Method for Comparison of Waterborne Petro-
leum Oils By Fluorescence Analysis
1.1 This practice covers the preservation of waterborne oil
D4489 Practices for Sampling of Waterborne Oils
samples from the time of collection to the time of analysis.
Information is provided to ensure sample integrity and to avoid
3. Terminology
contamination and to minimize microbial degradation.
3.1 Definitions—For definitions of terms used in this
1.2 The practice is for controlled field or laboratory condi-
practice, refer to Terminology D1129.
tions and specifies thorough preparation of equipment and
precise operation.Where these details must be compromised in
4. Summary of Practice
a field emergency, nonstandard simplifications are recom-
4.1 Special types of sample containers and shipping con-
mended that will minimize or eliminate consequent errors.
tainers are recommended. Samples may be of several types: tar
NOTE 1—Procedures for the analysis of oil spill samples are Practices
balls, collected oil, oil-water mixtures, emulsions, and oil and
D3326,D3415,D3650,andD4489,andTestMethodsD3327,D3328,and
water on collecting devices such as silanized glass cloth,
D3414. A guide to the use of ASTM test methods for the analysis of oil
TFE-fluorocarbon polymer, or other materials. Instructions are
spill samples is found in Practice D3415.
given for the care of samples to minimize changes due to
1.3 This standard does not purport to address all of the
autoxidation and microbial attack between the time of sam-
safety concerns, if any, associated with its use. It is the
pling and the time of analysis. Services available for transpor-
responsibility of the user of this standard to establish appro-
tation of samples are described.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
5. Apparatus
2. Referenced Documents
5.1 Sample Containers—Borosilicate glass containers that
have been thoroughly cleaned are preferable. All glass
2.1 ASTM Standards:
containers, new or used, must be thoroughly cleaned and
D1129 Terminology Relating to Water
washed prior to use. The cleaning steps consist of an initial
D3326 Practice for Preparation of Samples for Identification
wash with a warm aqueous detergent mixture followed by six
of Waterborne Oils
hot tap water rinses, two rinses with reagent water, a rinse with
D3327 Test Method for Analysis of Selected Elements in
reagent-grade acetone, and a final rinse with a solvent such as
Waterborne Oils (Withdrawn 1993)
pentane, hexane, cyclohexane, dichloromethane, or chloroform
D3328 Test Methods for Comparison of Waterborne Petro-
followed by drying in a clean oven at 105°C or hotter for 30
leum Oils by Gas Chromatography
min. If the glassware requires cleaning under field conditions,
D3414 Test Method for Comparison of Waterborne Petro-
it should be washed with warm aqueous detergent followed by
leum Oils by Infrared Spectroscopy
extensive water rinsing.Asolvent rinse with acetone should be
D3415 Practice for Identification of Waterborne Oils
made, if possible, followed by lengthy air drying to remove
residual solvent. (Warning—For safety reasons, the use of
This practice is under the jurisdiction of ASTM Committee D19 on Water and
pentane, hexane, or cylcohexane is recommended over use of
is the direct responsibility of Subcommittee D19.06 on Methods for Analysis for
dichloromethane or carbon tetrachloride.)
Organic Substances in Water.
Current edition approved Feb. 15, 2013. Published March 2013. Originally
NOTE 2—Hot reagent water rinses are advisable where hot tap water
approved in 1974. Last previous edition approved in 2002 as D3325 – 90 (2006).
might reintroduce contamination.
DOI: 10.1520/D3325-90R13.
5.1.1 Plastic containers are not acceptable since volatile
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
hydrocarbons diffuse readily through many commercial plastic
Standards volume information, refer to the standard’s Document Summary page on
containers or may be absorbed into the plastic. In addition, the
the ASTM website.
plasticizer may dissolve in the sample causing misleading
The last approved version of this historical standard is referenced on
www.astm.org. results.
*A Summary of Changes section appears at the end of this standard
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D3325 − 90 (2013)
5.1.2 Metal containers usually should be avoided because 7. Sampling
the nickel and vanadium determinations could be invalidated
7.1 Collect a representative sample in accordance with
by introduction of metal from the can.
Practices D4489.
5.1.3 When field expedients must be employed, an empty
7.2 Sample containers should be carefully prepared as
container of each type used should be included in the shipment
described in 5.1.
to the laboratory to be used as a blank to measure inadvertent
contamination.
8. Preservation of Samples
5.2 Closures—Proper choice of closures is critical to avoid
8.1 Protection Against Autoxidation—Treat the sample con-
contamination and to preserve sample. Use caps with
tainer to displace air and store in a dark area in a refrigerator.
aluminum-coated or TFE-fluorocarbon-coated cardboard in-
Nitrogen or carbon dioxide can be used as inert gases to
serts. Aluminum foil sheet should not be used. Inserts of
displace air.
TFE-fluorocarbon sheet, however, are acceptable.
8.2 Protection Against Microbial Attack—Maintain refrig-
5.3 Refrigerator, explosion-proof at about 4 to 5°C.
eration (4 to 5°C) once samples are received in the laboratory.
5.4 Shipping Containers—Sturdy cartons or wooden boxes
should be used. These should be sufficiently large so the
9. Shipment of Samples
sample containers are adequately surrounded by absorbent
9.1 Th
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