ASTM E2274-09
(Test Method)Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants
Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants
SIGNIFICANCE AND USE
The procedure in this test method is used to evaluate the activity of a test reagent (antimicrobial agent/active ingredient) or formulation in the reduction or complete kill of the bacterial population in fabric and wash water following a single wash.
SCOPE
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in top-loading automatic clothes washing operations. This test method is designed predominantly to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.
Note 1—This test method does not evaluate sanitizing/disinfectant laundry detergent/additives for use in front-loading, low water volume automatic clothes washing operations.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
Note 2—In this method, metric units are used for all applications, except for distance in which case inches are used.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
General Information
Relations
Buy Standard
Standards Content (Sample)
NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
Contact ASTM International (www.astm.org) for the latest information
Designation: E2274 − 09
StandardTest Method for
1
Evaluation of Laundry Sanitizers and Disinfectants
This standard is issued under the fixed designation E2274; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2.2 Other Documents:
AATCC Test Method 70-1997Water Repellency; Tumble
1.1 This test method is designed to evaluate sanitizing/
3
Jar Dynamic Absorption Test
disinfectant laundry detergents/additives for use in top-loading
DIS/TSS 13 Laundry Additives—Disinfection and
automatic clothes washing operations. This test method is
Sanitization, U.S. Environmental Protection Agency, Of-
designed predominantly to provide testing with representative
4
fice of Pesticide Programs, April 1980
vegetative bacteria but can also be designed to accommodate
the testing of fungi and viruses.
3. Terminology
NOTE 1—This test method does not evaluate sanitizing/disinfectant
laundry detergent/additives for use in front-loading, low water volume
3.1 Definitions:
automatic clothes washing operations.
3.1.1 active antimicrobial ingredient—a substance added to
1.2 Knowledge of microbiological techniques is required a formulation intended specifically for the inhibition or inac-
for these procedures. tivation of microorganisms.
3.1.2 antimicrobial agent(s)—an active ingredient designed
1.3 The values stated in SI units are to be regarded as
to suppress the growth or action of microorganisms.
standard. No other units of measurement are included in this
standard.
3.1.3 carrier count control—procedure used to determine
the initial number of microorganisms on a fabric carrier
NOTE 2—In this method, metric units are used for all applications,
following the inoculation and drying procedure.
except for distance in which case inches are used.
3.1.4 diluent—sterile deionized water, sterile distilled water
1.4 This standard does not purport to address all of the
or sterile synthetic AOAC hard water that may be used to
safety concerns, if any, associated with its use. It is the
prepare the active test formulation, vehicle control or product
responsibility of the user of this standard to establish appro-
control for use in the test procedure.
priate safety and health practices and determine the applica-
bility of regulatory limitations prior to use.
3.1.5 diluted product solution—test formulation, vehicle
control, or product control diluted to use concentration.
2. Referenced Documents
3.1.6 neutralization—aprocessthatresultsinquenchingthe
2
antimicrobial activity of a test formulation. This may be
2.1 ASTM Standards:
achieved by dilution of the test formulation(s) to reduce the
D1193Specification for Reagent Water
concentration of the antimicrobials, or through the use of
E177Practice for Use of the Terms Precision and Bias in
chemical agents, called neutralizers, to suppress antibacterial
ASTM Test Methods
activity.
E691Practice for Conducting an Interlaboratory Study to
Determine the Precision of a Test Method
3.1.7 numbers control—in assessing sanitizer level
E1054Test Methods for Evaluation of Inactivators of Anti-
performance, procedure used to determine the number of
microbial Agents
microorganisms remaining on the fabric carriers and in the
wash water following the test procedure in the presence of the
diluent.Thismayalsobeperformedusingdiluentorphosphate
buffer dilution water with surfactant.
1
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Nov. 1, 2009. Published December 2009. Originally
3
approved in 2003. Last previous edition approved in 2003 as E2247–03. DOI: Available from American Association of Textile Chemists and Colorists
10.1520/E2274-09. (AATCC), P.O. Box 12215, Research Triangle Park, NC 27709, http://
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or www.aatcc.org.
4
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Available from United States Environmental Protection Agency (EPA), Ariel
Standards volume information, refer to the standard’s Document Summary page on Rios Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460, http://
the ASTM website. www.epa.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E2274 − 09
3.1.8 product control—a formulation with or without an
active ingredient(s) used for comparison to the test formula-
tion.
3.1.9 t
...
This document is not anASTM standard and is intended only to provide the user of anASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:E2274–03 Designation:E2274–09
Standard Test Method for
1
Evaluation of Laundry Sanitizers and Disinfectants
This standard is issued under the fixed designation E2274; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 Thistestmethodisdesignedtoevaluatesanitizing/disinfectantlaundrydetergents/additivesforuseintop-loadingautomatic
clothes washing operations. This test method is designed predominantly to provide testing with representative vegetative bacteria
but can also be designed to accommodate the testing of fungi and viruses.
NOTE 1—This test method does not evaluate sanitizing/disinfectant laundry detergent/additives for use in front-loading, low water volume automatic
clothes washing operations.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3In this method metric units are used for all applications, except for distance in which case inches are used.
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
NOTE 2—In this method, metric units are used for all applications, except for distance in which case inches are used.
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
2.2 Other Documents:
The American Association of Textile Chemists And Colorists (AATCC) Test Method 70-1997AATCC Test Method
70-1997 Water Repellency; Tumble Jar Dynamic Absorption Test
3
Official Methods of Analysis of AOAC InternationalAOAC, Washington, D.C., Chapter 6: Disinfectants, 17th ed., 2000.
DIS/TSS 13 Laundry Additives—Disinfection and Sanitization, U.S. Environmental Protection Agency, Office of Pesticide
Programs, April 1980
Federal Standard 40 CFRPart 160, Good Laboratory Practice Standards
4
Canadian Pest Management Regulatory StandardsTrade Memorandum T-1-215
3. Terminology
3.1 Definitions:
3.1.1 active antimicrobial ingredient—asubstanceaddedtoaformulationintendedspecificallyfortheinhibitionorinactivation
of microorganisms.
3.1.2 antimicrobial agent(s)—an active ingredient designed to suppress the growth or action of microorganisms.
1
ThistestmethodisunderthejurisdictionofASTMCommitteeE35onPesticidesandAlternativeControlAgentsandisthedirectresponsibilityofSubcommitteeE35.15
on Antibacterial Agents.
Current edition approved Dec. 1, 2003. Published January 2004. DOI: 10.1520/E2274-03.on Antimicrobial Agents.
Current edition approved Nov. 1, 2009. Published December 2009. Originally approved in 2003. Last previous edition approved in 2003 as E2247–03. DOI:
10.1520/E2274-09.
2
ForreferencedASTMstandards,visittheASTMwebsite,www.astm.org,orcontactASTMCustomerServiceatservice@astm.org.For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
Available from U.S. Government Printing Office Superintendent of Documents, 732 N. Capitol St., NW, Mail Stop: SDE, Washington, DC 20401.
3
Available from American Association of Textile Chemists and Colorists (AATCC), P.O. Box 12215, Research Triangle Park, NC 27709, http://www.aatcc.org.
4
Fabric #400 8-1978) obtained from Test Fabric, Inc., P.O. Box 26, West Pittson, PA 18643 or supplier of comparable specifications.
4
Available from United States Environmental Protection Agency (EPA), Ariel Rios Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460, http://www.epa.gov.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1
---------------------- Page: 1 ----------------------
E2274–09
3.1.3 carrier count control—procedureusedtodeterminetheinitialnumberofmicroorganismsonafabriccarrierfollowingthe
inoculation and drying procedure.
3.1.4 diluent—sterile deionized water, sterile distilled water or steri
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.