Standard Test Method for Evaluation of Laundry Sanitizers and Disinfectants for Use in High Efficiency Washing Operations

SIGNIFICANCE AND USE
5.1 The procedure in this test method is used to evaluate the effectiveness of a test reagent (antimicrobial agent/active ingredient) or formulation to reduce or completely kill bacterial populations on contaminated fabrics and in wash water following a single wash under simulated low wash volume conditions. The water to fabric ratio in common front loading machines is dynamic and varies by region and machine used. The proper water to fabric ratio and temperature for the worse-case scenario for product use should be determined and documented prior to testing.
SCOPE
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in front loading high efficiency (HE) automatic clothes washing operations that typically utilize very low wash water volumes. This test method is designed to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi and viruses.  
1.2 This test method is intended to compliment Test Method E2274 and is to be used in the cases where this test method is determined to be the worse case scenario for product usage.
Note 1: Test Method E2274 is the recommended method to evaluate sanitizing/disinfectant laundry detergent/additives for use in traditional high wash water volume automatic clothes washing operations.  
1.3 Knowledge of microbiological techniques is required for these procedures.  
1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow them where appropriate (see section 40 CFR, 160 or as revised.  
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
Note 2: In this test method, metric units are used for all applications, except for distance, in which case inches are used.  
1.6 Appropriate modifications to the test method may be required when the testing organisms are not specified herein.  
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Standards Content (Sample)

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E2406 − 16
Standard Test Method for
Evaluation of Laundry Sanitizers and Disinfectants for Use
1
in High Efficiency Washing Operations
This standard is issued under the fixed designation E2406; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 2. Referenced Documents
2
2.1 ASTM Standards:
1.1 This test method is designed to evaluate sanitizing/
D1193Specification for Reagent Water
disinfectant laundry detergents/additives for use in front load-
E177Practice for Use of the Terms Precision and Bias in
ing high efficiency (HE) automatic clothes washing operations
ASTM Test Methods
that typically utilize very low wash water volumes. This test
E691Practice for Conducting an Interlaboratory Study to
method is designed to provide testing with representative
Determine the Precision of a Test Method
vegetative bacteria but can also be designed to accommodate
E1054Test Methods for Evaluation of Inactivators of Anti-
the testing of fungi and viruses.
microbial Agents
1.2 ThistestmethodisintendedtocomplimentTestMethod
E2274Test Method for Evaluation of Laundry Sanitizers
E2274 and is to be used in the cases where this test method is
and Disinfectants
determined to be the worse case scenario for product usage.
E2756Terminology Relating toAntimicrobial andAntiviral
Agents
NOTE 1—Test Method E2274 is the recommended method to evaluate
sanitizing/disinfectant laundry detergent/additives for use in traditional
2.2 Other Standards:
high wash water volume automatic clothes washing operations.
AATCC70WaterRepellency;TumbleJarDynamicAbsorp-
3
1.3 Knowledge of microbiological techniques is required tion Test
OSCPP 810.2400: Disinfectants and Sanitizers for Use on
for these procedures.
4
Fabrics and Textiles – Efficacy Data Recommendations
1.4 It is the responsibility of the investigator to determine
5
40 CFR, Part 160Good Laboratory Practice Standards
whether Good Laboratory Practices (GLP) are required and to
follow them where appropriate (see section 40 CFR, 160 or as
3. Terminology
revised.
3.1 For definitions of terms used in this test method refer to
1.5 The values stated in SI units are to be regarded as
Terminology E2756.
standard. No other units of measurement are included in this
3.2 Definitions of Terms Specific to This Standard:
standard.
3.2.1 active antimicrobial ingredient—a substance added to
NOTE 2—In this test method, metric units are used for all applications,
a formulation intended specifically for the inhibition or inac-
except for distance, in which case inches are used.
tivation of microorganisms.
1.6 Appropriate modifications to the test method may be
3.2.2 antimicrobial agent(s)—an active ingredient designed
required when the testing organisms are not specified herein.
to suppress the growth or action of microorganisms.
1.7 This standard does not purport to address all of the
safety concerns, if any, associated with its use. It is the
responsibility of the user of this standard to establish appro-
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
priate safety and health practices and determine the applica-
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
bility of regulatory limitations prior to use.
Standards volume information, refer to the standard’s Document Summary page on
the ASTM website.
3
Available from American Association of Textile Chemists and Colorists
(AATCC), P.O. Box 12215, Research Triangle Park, NC 27709, http://
1
This test method is under the jurisdiction of ASTM Committee E35 on www.aatcc.org.
4
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct Available from United States Environmental Protection Agency (EPA), Ariel
responsibility of Subcommittee E35.15 on Antimicrobial Agents. Rios Bldg., 1200 Pennsylvania Ave., NW, Washington, DC 20460, http://
Current edition approved April 15, 2016. Published May 2016. Originally www.epa.gov.
5
approved in 2004. Last previous edition approved in 2009 as E2406–09. DOI: Available from U.S. Government Publishing Office Bookstore 710 North
10.1520/E2406-16. Capitol Street N.W. Washington, DC, http://www.gpo.gov/about/bookstore.htm
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2406 − 16
3.2.3 carrier count control—procedure used to determine
the initial number of microorga
...

This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E2406 − 09 E2406 − 16
Standard Test Method for
Evaluation of Laundry Sanitizers and Disinfectants for Use
1
in High Efficiency Washing Operations
This standard is issued under the fixed designation E2406; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method is designed to evaluate sanitizing/disinfectant laundry detergents/additives for use in front loading high
efficiency (HE) automatic clothes washing operations that typically utilize very low wash water volumes. This test method is
designed to provide testing with representative vegetative bacteria but can also be designed to accommodate the testing of fungi
and viruses.
NOTE 1—Test Method E2274 is the recommended method to evaluate sanitizing/disinfectant laundry detergent/additives for use in traditional high wash
water volume automatic clothes washing operations.
1.2 This test method is intended to compliment Test Method E2274 and is to be used in the cases where this test method is
determined to be the worse case scenario for product usage.
NOTE 1—Test Method E2274 is the recommended method to evaluate sanitizing/disinfectant laundry detergent/additives for use in traditional high wash
water volume automatic clothes washing operations.
1.3 Knowledge of microbiological techniques is required for these procedures.
1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practices (GLP) are required and to follow
them where appropriate (see section 40 CFR, 160 or as revised.
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
NOTE 2—In this test method, metric units are used for all applications, except for distance, in which case inches are used.
1.6 Appropriate modifications to the test method may be required when the testing organisms are not specified herein.
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
2. Referenced Documents
2
2.1 ASTM Standards:
D1193 Specification for Reagent Water
E177 Practice for Use of the Terms Precision and Bias in ASTM Test Methods
E691 Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents
E2274 Test Method for Evaluation of Laundry Sanitizers and Disinfectants
E2756 Terminology Relating to Antimicrobial and Antiviral Agents
1
This test method is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Nov. 1, 2009April 15, 2016. Published December 2009May 2016. Originally approved in 2004. Last previous edition approved in 20042009 as
E2406 – 04.E2406 – 09. DOI: 10.1520/E2406-09.10.1520/E2406-16.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1

---------------------- Page: 1 ----------------------
E2406 − 16
2.2 AATCC Standard:Other Standards:
3
AATCC 70-199770 Water Repellency; Tumble Jar Dynamic Absorption Test
4
OSCPP 810.2400 : Disinfectants and Sanitizers for Use on Fabrics and Textiles – Efficacy Data Recommendations
5
40 CFR, Part 160 Good Laboratory Practice Standards
2.3 EPA Standard:
DIS/TSS 13 Laundry Additives—Disinfection and Sanitization, U.S. Environmental Protection Agency, Office of Pesticide
4
Programs, April 1980
3. Terminology
3.1 For definitions of terms used in this test method refer to Terminology E2756.
3.2 Definitions:Definitions of Terms Specific to This Standard:
3.2.1 active antimicrobial ingredient—a substance added to a formulation intended specifically for the inhibition or inactivation
of microorganisms.
3.2.2 antimicrobial agent(s)—an active ingredient designed
...

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