ASTM E2966-14

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Designation: E2966 − 14
Standard Test Method for
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Quantitative Assessment of Sanitizing Solutions for Carpet
This standard is issued under the fixed designation E2966; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.1.2 carpet sanitizer, n—chemical solution that reduces
biocontaminant levels on carpet when applied at the recom-
1.1 This test method is designed to evaluate quantitatively
mended dilution and contact time specified on the product
the antibacterial and antifungal activity of solutions for sani-
label.
tizing carpets.
3.1.3 sanitizer, n—chemical or physical agent(s) used to
1.2 Efficacy is reported as the log reduction in viable
reduce the number of microorganisms to a level judged to be
bacteria and fungi.
appropriate for a defined purpose and/or claim.
1.3 The bacteria used in the test are Staphylococcus aureus,
3.1.3.1 Discussion—The US EPA regulates sanitizers used
Enterobacter aerogenes, and Pseudomonas aeruginosa. The
on porous and non-porous surfaces. EPA 810 Guidelines
mold used is Aspergillus brasiliensis.
provide a description of each category, the required test
1.4 Knowledge of microbiological techniques is required
method, test conditions, and performance criteria. EPA
for this test method.
810.2400 (f) describes requirements for testing carpet sanitiz-
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1.5 Units—The values stated in SI units are to be regarded
ers.
asstandard.Nootherunitsofmeasurementareincludedinthis 3.1.3.2 Discussion—In the context of this method effective
standard.
sanitization reduces the number of microorganisms to levels
considered safe as determined by public health codes or
1.6 This standard does not purport to address all of the
regulations.
safety concerns, if any, associated with its use. It is the
3.1.4 vacuum extraction unit, n—machinefordeepcleaning
responsibility of the user of this standard to establish appro-
carpetthatdeliversasprayofcleaningsolution,providesbrush
priate safety and health practices and determine the applica-
agitation of the pile, and recovers soil and cleaning solution
bility of regulatory limitations prior to use.
under vacuum.
2. Referenced Documents
4. Summary of Test Method
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2.1 ASTM Standards:
4.1 In this test method, the efficacy of solutions intended to
E1054Test Methods for Evaluation of Inactivators of Anti-
have a sanitizing effect on carpet are quantitatively evaluated.
microbial Agents
Carpetsamplecouponsarecutfromalargerfieldofcarpetand
E2471Test Method for Using Seeded-Agar for the Screen-
re-embedded within the field. The carpet coupons are inocu-
ing Assessment of Antimicrobial Activity In Carpets
lated with microorganisms followed by a drying period. After
the inoculum-drying period, the sanitizing solution is applied
3. Terminology
tothecarpetcouponsfollowedbyscrubbing.Additionalcarpet
3.1 Definitions:
coupons are spray treated and scrubbed with a inert solution.
3.1.1 carpet cleaner/sanitizer, n—solution that cleans em-
After the chemical contact period, carpet coupons are asepti-
bedded soil from the carpet fiber and reduces biocontaminant
cally removed and placed into neutralizing broth. Each neu-
levelsoncarpetwhenappliedattherecommendeddilutionand
tralizing broth with carpet coupon is placed into a ultrasonic
contact time specified on the product label.
bathfor1minfollowedby1minofwristactionshaking.Serial
dilutions are performed on each sample followed by plating
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(pour or spread plates or other standard for enumerating viable
This test method is under the jurisdiction of ASTM Committee E35 on
Pesticides, Antimicrobials, and Alternative Control Agents and is the direct
cells). Log reduction of the viable cell counts recovered from
responsibility of Subcommittee E35.15 on Antimicrobial Agents.
“scrubbed-controls” versus viable cell counts recovered from
Current edition approved May 15, 2014. Published June 2014. DOI: 10.1520/
the sanitizer-treated carpets are recorded.
E2966–14
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For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
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Standards volume information, refer to the standard’s Document Summary page on http://www.epa.gov/ocspp/pubs/frs/publications/Test_Guidelines/
the ASTM website. series810.htm
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E2966 − 14
5. Significance and Use 7.1.3 Recommendedapplicationrates(volumeperunitarea,
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thatis,ml/m )areextrapolatedandreportedfromtheproposed
5.1 Carpet,whenexposedtotheenvir
...