ASTM C1338-08
(Test Method)Standard Test Method for Determining Fungi Resistance of Insulation Materials and Facings
Standard Test Method for Determining Fungi Resistance of Insulation Materials and Facings
SIGNIFICANCE AND USE
The type of materials used in the manufacture of insulation products and the type of membrane used to face these products can sometimes affect fungi sustenance in the presence of high humidity.
This test method is used to determine the relative ability of an insulation and its facing to support or resist fungal growth under conditions favorable for their development.
This test method uses a comparative material to determine the relative ability of a material to support fungal growth. In some specialized product areas, it is required that no growth take place. In such cases, the use of the comparative material is omitted and the pass/fail criterion is based upon growth.
SCOPE
1.1 This test method covers the determination of the ability of new insulation materials and their facings to support fungal growth.
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
1.3 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.
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Designation: C1338 − 08
StandardTest Method for
Determining Fungi Resistance of Insulation Materials and
1
Facings
This standard is issued under the fixed designation C1338; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope 3.2 Environmental Chamber or Cabinet—Equipment for
this test method shall maintain a temperature of 82.4 to 86°F
1.1 This test method covers the determination of the ability
(28 to 30°C) and a relative humidity of 95% (64%).
of new insulation materials and their facings to support fungal
Provisions shall be made to prevent condensation from drip-
growth.
ping on the test specimen.There shall be free circulation of air
1.2 This standard does not purport to address all of the
around the test chamber.
safety concerns, if any, associated with its use. It is the
3.3 Atomizer—A chromatography atomizer capable of pro-
responsibility of the user of this standard to establish appro-
2
viding 100000 6 20000 spores/in. (15000 6 3000 spores/
priate safety and health practices and determine the applica-
2
cm ) shall be used for inoculation.
bility of regulatory limitations prior to use.
1.3 The values stated in inch-pound units are to be regarded
3.4 Autoclavable Biohazard Bags, or metal pan able to
as standard. The values given in parentheses are mathematical
withstand autoclaving.
conversions to SI units that are provided for information only
and are not considered standard. 4. Reagents and Materials
4.1 Purity of Water—Unless otherwise specified, references
2. Significance and Use
to water shall be understood to mean sterile distilled water or
2.1 The type of materials used in the manufacture of
water of equal purity.
insulation products and the type of membrane used to face
4.2 Inoculum:
these products can sometimes affect fungi sustenance in the
2
Fungi ATCC
presence of high humidity.
Aspergillus niger 9642
Aspergillus versicolor 11 730
2.2 Thistestmethodisusedtodeterminetherelativeability
Penicillium funiculosum 11 797
ofaninsulationanditsfacingtosupportorresistfungalgrowth
Chaetomium globosum 6205
under conditions favorable for their development. Aspergillus flavus 9643
4.3 Cultures—Maintain cultures of the Aspergillus fungi
2.3 This test method uses a comparative material to deter-
separately on Czapek Dox agar (see Note 1). Culture the
minetherelativeabilityofamaterialtosupportfungalgrowth.
Chaetomiumglobosumonstripsofcellulosefilterpaperonthe
Insomespecializedproductareas,itisrequiredthatnogrowth
surfaceofCzapekDoxagar.MaintainthePenicilliumfungion
take place. In such cases, the use of the comparative material
Sabouraud Dextrose agar. The stock cultures may be kept for
is omitted and the pass/fail criterion is based upon growth.
not more than 4 months at 43 6 7°F (6 6 4°C) at which time
subcultures shall be made, and new stocks selected from the
3. Apparatus
subcultures. If genetic or physiological changes occur, obtain
3.1 Glassware—Sterile disposable petri dishes, 4 or 6 in.
new cultures. Incubate subcultures used for preparing new
(100 or 150 mm) by 0.6 or 0.75 in. (15 or 20 mm) in size are
stock cultures or the spore suspension at 86 6 4°F (30 6 2°C)
preferred. For larger specimens, trays of borosilicate glass or
for 5 days or longer.
baking dishes up to 16 by 20 in. (400 by 600 mm) in size may
be used. NOTE 1—This media is readily available from any science/
microbiological supply house.
1
ThistestmethodisunderthejurisdictionofASTMCommitteeC16onThermal
2
InsulationandisthedirectresponsibilityofSubcommitteeC16.31onChemicaland The sole source of supply of the cultures known to the committee at this time
Physical Properties. is American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville,
CurrenteditionapprovedJune1,2008.PublishedJuly2008.Originallyapproved MD20852.Ifyouareawareofalternativesuppliers,pleaseprovidethisinformation
in 1996. Last previous edition approved in 2000 as C1338–00. DOI: 10.1520/ to ASTM International Headquarters. Your comments will receive careful consid-
1
C1338-08. erationatameetingoftheresponsibletechnicalcommittee, whichyoumayattend.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
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C1338 − 08
5. Specimens the spore pellet. Resuspend the residue in 50 mL of sterile
water and centrifuge. (It may be necessary to add a small
5.1 Viability Specimens—Determine the viability of the
quantity of nontoxic wetting agent, not to exceed 0.10 g/L, to
spore suspension during incubation with these controls: with
prevent clumping o
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation:C1338–00 Designation: C 1338 – 08
Standard Test Method for
Determining Fungi Resistance of Insulation Materials and
1
Facings
This standard is issued under the fixed designation C 1338; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope
1.1 This test method covers the determination of the ability of new insulation materials and their facings to support fungal
growth.
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory
limitations prior to use.
1.3The values stated in inch-pound units are to be regarded as the standard. The values given in parentheses are provided for
information only.
1.3 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical
conversions to SI units that are provided for information only and are not considered standard.
2. Significance and Use
2.1 The type of materials used in the manufacture of insulation products and the type of membrane used to face these products
can sometimes affect fungi sustenance in the presence of high humidity.
2.2 This test method is used to determine the relative ability of an insulation and its facing to support or resist fungal growth
under conditions favorable for their development.
2.3 Thistestmethodusesacomparativematerialtodeterminetherelativeabilityofamaterialtosupportfungalgrowth.Insome
specialized product areas, it is required that no growth take place. In such cases, the use of the comparative material is omitted
and the pass/fail criterion is based upon growth.
3. Apparatus
3.1 Glassware—Steriledisposablepetridishes,4or6in.(100or150mm)by0.6or0.75in.(15or20mm)insizearepreferred.
For larger specimens, trays of borosilicate glass or baking dishes up to 16 by 20 in. (400 by 600 mm) in size may be used.
3.2 Environmental Chamber or Cabinet —Equipment for this test method shall maintain a temperature of 82.4 to 86°F (28 to
30°C) and a relative humidity of 95 % (64 %). Provisions shall be made to prevent condensation from dripping on the test
specimen. There shall be free circulation of air around the test chamber.
2 2
3.3 Atomizer—A chromatography atomizer capable of providing 100 000 6 20 000 spores/in. (15 000 6 3000 spores/cm )
shall be used for inoculation.
3.4 Autoclavable Biohazard Bags , or metal pan able to withstand autoclaving.
4. Reagents and Materials
4.1 PurityofWater—Unlessotherwisespecified,referencestowatershallbeunderstoodtomeansteriledistilledwaterorwater
of equal purity.
4.2 Inoculum:
2
Fungi ATCC
Aspergillus niger 9642
Aspergillus versicolor 11 730
1
This test method is under the jurisdiction of ASTM Committee C016 on Thermal Insulation and is the direct responsibility of Subcommittee C16.31 on Chemical and
Physical Properties.
Current edition approved May 10.2000. Published August 2000.
1
This test method is under the jurisdiction of ASTM Committee C16 on Thermal Insulation and is the direct responsibility of Subcommittee C16.31 on Chemical and
Physical Properties.
Current edition approved June 1, 2008. Published July 2008. Originally approved in 1996. Last previous edition approved in 2000 as C 1338–00.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
1
---------------------- Page: 1 ----------------------
C1338–08
Penicillium funiculosum 11 797
Chaetomium globosum 6205
Aspergillus flavus 9643
4.3 Cultures—Maintain cultures of theAspergillus fungi separately on Czapek Dox agar (see Note 1). Culture the Chaetomium
globosum on strips of Whatman 500 cellulose filter paper on the surface of Czapek Dox agar. Maintain the Penicillium fungi on
Sabouraud Dextrose agar. The stock cultures may be kept for not more than 4 months at 43 6 7°F (6 6 4°C) at which time
subcultures shall be made, and new stocks selected from the subcultures. Obtain new cultures from ATCC annually.If genetic or
physiological changes occur, obtain new cultures. Incubate subcultures used for preparing new stock cultures or the spore
suspension at 86 6 4°F (30 6 2°C) for 5 days or longer.
NOTE 1—This media is readily available from any sci
...
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