ASTM C1338-00

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation:C1338–00
Standard Test Method for
Determining Fungi Resistance of Insulation Materials and
Facings
This standard is issued under the fixed designation C 1338; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (e) indicates an editorial change since the last revision or reapproval.
1. Scope ping on the test specimen. There shall be free circulation of air
around the test chamber.
1.1 This test method covers the determination of the ability
3.3 Atomizer—A chromatography atomizer capable of pro-
of new insulation materials and their facings to support fungal
viding 100 000 6 20 000 spores/in. (15 000 6 3000 spores/
growth.
cm ) shall be used for inoculation.
1.2 This standard does not purport to address all of the
3.4 Autoclavable Biohazard Bags, or metal pan able to
safety concerns, if any, associated with its use. It is the
withstand autoclaving.
responsibility of the user of this standard to establish appro-
priate safety and health practices and determine the applica-
4. Reagents and Materials
bility of regulatory limitations prior to use.
4.1 Purity of Water—Unless otherwise specified, references
1.3 The values stated in inch-pound units are to be regarded
to water shall be understood to mean sterile distilled water or
as the standard. The values given in parentheses are provided
water of equal purity.
for information only.
4.2 Inoculum:
2. Significance and Use
Fungi ATCC
2.1 The type of materials used in the manufacture of
Aspergillus niger 9642
insulation products and the type of membrane used to face
Aspergillus versicolor 11 730
these products can sometimes affect fungi sustenance in the
Penicillium funiculosum 11 797
Chaetomium globosum 6205
presence of high humidity.
Aspergillus flavus 9643
2.2 This test method is used to determine the relative ability
ofaninsulationanditsfacingtosupportorresistfungalgrowth 4.3 Cultures—Maintain cultures of the Aspergillus fungi
separately on Czapek Dox agar (see Note 1). Culture the
under conditions favorable for their development.
2.3 This test method uses a comparative material to deter- Chaetomium globosum on strips of Whatman 500 filter paper
on the surface of Czapek Dox agar. Maintain the Penicillium
mine the relative ability of a material to support fungal growth.
In some specialized product areas, it is required that no growth fungi on Sabouraud Dextrose agar. The stock cultures may be
kept for not more than 4 months at 43 6 7°F (6 6 4°C) at
take place. In such cases, the use of the comparative material
which time subcultures shall be made, and new stocks selected
is omitted and the pass/fail criterion is based upon growth.
from the subcultures. Obtain new cultures from ATCC annu-
3. Apparatus
ally. Incubate subcultures used for preparing new stock cul-
3.1 Glassware—Sterile disposable petri dishes, 4 or 6 in. tures or the spore suspension at 86 6 4°F (30 6 2°C) for 5
days or longer.
(100 or 150 mm) by 0.6 or 0.75 in. (15 or 20 mm) in size are
preferred. For larger specimens, trays of borosilicate glass or
NOTE 1—This media is readily available from any science/
baking dishes up to 16 by 20 in. (400 by 600 mm) in size may
microbiological supply house.
be used.
5. Specimens
3.2 Environmental Chamber or Cabinet—Equipment for
this test method shall maintain a temperature of 82.4 to 86°F
5.1 Viability Specimens—Determine the viability of the
(28 to 30°C) and a relative humidity of 95 % (64 %).
spore suspension during incubation with these controls: with
Provisions shall be made to prevent condensation from drip-
each daily group of tests, place one piece of sterilized What-
2 2
man 500 filter paper, 1 in. (6.4 cm ) on each of two prepared
This test method is under the jurisdiction of ASTM Committee C016 on
Thermal Insulation and is the direct responsibility of Subcommittee C16.31 on
Chemical and Physical Properties. Available from American Type Culture Collection, 12301 Parklawn Drive,
Current edition approved May 10.2000. Published August 2000. Rockville, MD 20852.
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.
C1338
hardened Czapek Dox agar specimens in separate petri dishes. the resultant spore suspension shall contain 1 000 0006
Prepare a third petri dish with Sabouraud Dextrose agar. 200 000sporespermLasdeterminedwithacountingchamber.
5.2 Comparative Material—A white birch tongue depres-
Repeat the operation for each organism used in the test and
sor,0.75by6in.(20by150mm)orsouthernyellowpineisthe blend equal volumes of the resultant spore suspensions to
comparative item to determine the relative growth on speci-
obtain the final mixed spore suspension. The spore suspension
mens being tested. The choice of comparative item should be
may be prepared fresh each day or may be held at 43 6 7°F (6
noted. Refer to the appropriate materials standard for relevant
6 4°C) for not more than 28 days, or until the viability test
comparative materials.
indicates poor growth, or until growth appears in the sealed
storage bottle.
NOTE 2—The comparative item is chosen to reflect the building
construction material. In some cases, other material may be more relevant 6.2 Inoculation of Test Specimens, Comparative Material,
as a comparative item.
and Control Specimens—Precondition the chamber and its
contents at 866 4°F (30 6 2°C) and 956 4 % relative
5.3 Test Specimens:
humidity for at least 4 h. Place each test, comparative material,
5.3.1 Prepare triplicate specimens from each test sample.
and viability control specimen in separate sterile petri dishes.
5.3.1.1 If the test sample is of different construction on each
Inoculate each specimen with approximately 0.50 mL of spore
face, prepare triplicate specimens of each face in the up
suspension by spraying exposed
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