ASTM E1259-23
(Practice)Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
SIGNIFICANCE AND USE
5.1 Guide D6469 details the types of problems associated with uncontrolled microbial growth in fuels and fuel systems. Treatment with effective antimicrobial agents is one element of contamination control strategy.
5.2 The procedure should be used to evaluate the relative efficacy of microbicides in liquid fuels boiling below 390 °C. The effect of environmental conditions, such as a variety of fuel additives, metal surfaces, and climatology, are variables that can be included in specific tests using this protocol.
5.3 This practice addresses product performance issues only. Regulatory Agencies restrict and control the use of both pesticides (in the U.S.: 40 CFR 152) and fuel additives (40 CFR 79). Regardless of performance in this method, antimicrobials must only be used in compliance with applicable regulations. Specific industries, for example, the aviation industry, may place further restrictions on chemicals used for fuel treatment.
SCOPE
1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid fuels (as defined by Specification D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, D6751, and D7467), system deterioration, or both.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them where appropriate (40 CFR, 160), or as revised.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E1259 − 23
Standard Practice for
Evaluation of Antimicrobials in Liquid Fuels Boiling Below
1
390 °C
This standard is issued under the fixed designation E1259; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope* D1655 Specification for Aviation Turbine Fuels
3
D2069 Specification for Marine Fuels (Withdrawn 2003)
1.1 This practice is designed to evaluate antimicrobial
D2880 Specification for Gas Turbine Fuel Oils
agents for the prevention of microbially influenced deteriora-
D3699 Specification for Kerosine
tion of liquid fuels (as defined by Specification D396, D910,
D4814 Specification for Automotive Spark-Ignition Engine
D975, D1655, D2069, D2880, D3699, D4814, D6227, D6751,
Fuel
and D7467), system deterioration, or both.
D5465 Practices for Determining Microbial Colony Counts
1.2 Knowledge of microbiological techniques is required
from Waters Analyzed by Plating Methods
for these procedures.
D6227 Specification for Unleaded Aviation Gasoline Con-
taining a Non-hydrocarbon Component
1.3 It is the responsibility of the investigator to determine
whether Good Laboratory Practice (GLP) is required and to D6293 Test Method for Oxygenates and Paraffin, Olefin,
Naphthene, Aromatic(O-PONA) Hydrocarbon Types in
follow them where appropriate (40 CFR, 160), or as revised.
Low-Olefin Spark Ignition Engine Fuels by Gas Chroma-
1.4 The values stated in SI units are to be regarded as
3
tography (Withdrawn 2009)
standard. No other units of measurement are included in this
D6469 Guide for Microbial Contamination in Fuels and Fuel
standard.
Systems
1.5 This standard does not purport to address all of the
D6729 Test Method for Determination of Individual Com-
safety concerns, if any, associated with its use. It is the
ponents in Spark Ignition Engine Fuels by 100 Metre
responsibility of the user of this standard to establish appro-
Capillary High Resolution Gas Chromatography
priate safety, health, and environmental practices and deter-
D6733 Test Method for Determination of Individual Com-
mine the applicability of regulatory limitations prior to use.
ponents in Spark Ignition Engine Fuels by 50-Metre
1.6 This international standard was developed in accor-
Capillary High Resolution Gas Chromatography
dance with internationally recognized principles on standard-
D6751 Specification for Biodiesel Fuel Blendstock (B100)
ization established in the Decision on Principles for the
for Middle Distillate Fuels
Development of International Standards, Guides and Recom-
D6974 Practice for Enumeration of Viable Bacteria and
mendations issued by the World Trade Organization Technical
Fungi in Liquid Fuels—Filtration and Culture Procedures
Barriers to Trade (TBT) Committee.
D7463 Test Method for Adenosine Triphosphate (ATP) Con-
tent of Microorganisms in Fuel, Fuel/Water Mixtures, and
2. Referenced Documents
Fuel Associated Water
2
2.1 ASTM Standards:
D7464 Practice for Manual Sampling of Liquid Fuels, As-
D396 Specification for Fuel Oils
sociated Materials and Fuel System Components for
D910 Specification for Leaded Aviation Gasolines
Microbiological Testing
D975 Specification for Diesel Fuel
D7467 Specification for Diesel Fuel Oil, Biodiesel Blend
(B6 to B20)
D7687 Test Method for Measurement of Cellular Adenosine
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides,
Triphosphate in Fuel and Fuel-associated Water With
Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Sample Concentration by Filtration
Subcommittee E35.15 on Antimicrobial Agents.
D7978 Test Method for Determination of the Viable Aerobic
Current edition approved June 1, 2023. Published June 2023. Originally
approved in 1988. Last previous edition approved in 2018 as E1259 – 18. DOI: Microbial Content of Fuels and Associated Water—
10.1520/E1259-23.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or
contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
3
Standards volume information, refer to the standard’s Document Summary page on The last approved version of this historical standard is referenced on
the ASTM website. www.astm.org.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E1259 − 23
Thixotropic Gel Culture
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E1259 − 18 E1259 − 23
Standard Practice for
Evaluation of Antimicrobials in Liquid Fuels Boiling Below
1
390 °C
This standard is issued under the fixed designation E1259; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope*
1.1 This practice is designed to evaluate antimicrobial agents for the prevention of microbially influenced deterioration of liquid
fuels (as defined by Specification D396, D910, D975, D1655, D2069, D2880, D3699, D4814, D6227, D6751, and D7467), system
deterioration, or both.
1.2 Knowledge of microbiological techniques is required for these procedures.
1.3 It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) is required and to follow them
where appropriate (40 CFR, 160), or as revised.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of
regulatory limitations prior to use.
1.6 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
2. Referenced Documents
2
2.1 ASTM Standards:
D396 Specification for Fuel Oils
D910 Specification for Leaded Aviation Gasolines
D975 Specification for Diesel Fuel
D1655 Specification for Aviation Turbine Fuels
3
D2069 Specification for Marine Fuels (Withdrawn 2003)
D2880 Specification for Gas Turbine Fuel Oils
D3699 Specification for Kerosine
D4814 Specification for Automotive Spark-Ignition Engine Fuel
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Oct. 1, 2018June 1, 2023. Published October 2018June 2023. Originally approved in 1988. Last previous edition approved in 20162018 as
E1259 – 16.E1259 – 18. DOI: 10.1520/E1259-18.10.1520/E1259-23.
2
For referenced ASTM standards, visit the ASTM website, www.astm.org, or contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM Standards
volume information, refer to the standard’s Document Summary page on the ASTM website.
3
The last approved version of this historical standard is referenced on www.astm.org.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E1259 − 23
D5465 Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
D6227 Specification for Unleaded Aviation Gasoline Containing a Non-hydrocarbon Component
D6293 Test Method for Oxygenates and Paraffin, Olefin, Naphthene, Aromatic(O-PONA) Hydrocarbon Types in Low-Olefin
3
Spark Ignition Engine Fuels by Gas Chromatography (Withdrawn 2009)
D6469 Guide for Microbial Contamination in Fuels and Fuel Systems
D6729 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 100 Metre Capillary High
Resolution Gas Chromatography
D6733 Test Method for Determination of Individual Components in Spark Ignition Engine Fuels by 50-Metre Capillary High
Resolution Gas Chromatography
D6751 Specification for Biodiesel Fuel Blendstock (B100) for Middle Distillate Fuels
D6974 Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
D7463 Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel
Associated Water
D7464 Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological
Testing
D7467 Specification for Diesel Fuel Oil, Biodiesel Blend (B6 to B20)
D7687 Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With S
...
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