SIST EN ISO 6865:2001
(Main)Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)
Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg.
NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Futtermittel - Bestimmung des Rohfasergehaltes - Verfahren mit Zwischenfiltration (ISO/FDIS 6865:2000)
Diese Internationale Norm legt ein Verfahren zur Bestimmung des Rohfasergehaltes mit Zwischenfiltration fest. Es wird sowohl ein manuelles Verfahren als auch ein halbautomatisches Verfahren beschrieben. Dieses Verfahren ist auf Futtermittel mit einem Rohfasergehalt von größer als 10 g/kg anwendbar. ANMERKUNG Für Futtermittel mit einem Rohfasergehalt kleiner als oder gleich 10 g/kg darf das in ISO 6541 : 1981 beschriebene Verfahren angewandt werden. Diese Internationale Norm ist auch auf Getreide und Hülsenfrüchte anwendbar.
Aliments des animaux - Détermination de la teneur en cellulose brute - Méthode avec filtration intermédiaire (ISO/FDIS 6865:2000)
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg.
NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Krmila - Določevanje surovih vlaken - Metoda z vmesno filtracijo (ISO 6865:2000)
General Information
- Status
- Published
- Publication Date
- 31-Jan-2001
- Technical Committee
- KŽP - Agricultural food products
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 01-Feb-2001
- Due Date
- 01-Feb-2001
- Completion Date
- 01-Feb-2001
Overview
EN ISO 6865:2000 defines a laboratory method for the determination of crude fibre in animal feedingstuffs using a procedure with intermediate filtration. The standard describes both a manual and a semi-automatic procedure and is applicable to samples with crude fibre content greater than 10 g/kg. It is also explicitly applicable to cereals and pulses. For feeds with crude fibre ≤ 10 g/kg, ISO 6541 is recommended.
The method is widely used in feed-testing laboratories for routine quality control and regulatory compliance because it provides a reproducible, traceable measurement of the insoluble cellulosic fraction after acid and alkali treatment.
Key Topics
- Scope and applicability: animal feedingstuffs, cereals and pulses; intended for samples with crude fibre > 10 g/kg.
- Principle: the test portion is subjected to boiling dilute sulfuric acid followed by boiling potassium hydroxide. Residues are separated by filtration, washed, dried and ashed; the mass loss on ashing corresponds to crude fibre content.
- Procedures: both manual and semi‑automatic workflows are provided to accommodate different laboratory capacities and equipment.
- Typical test steps:
- Grind and prepare representative test sample (passes 1 mm sieve).
- Weigh test portion (~1 g) and carry out acid digestion, intermediate filtration, and washing.
- Perform alkali digestion, second filtration and washing.
- Dry residue (oven, 130 °C), weigh, then ash (muffle furnace, about 500 ± 25 °C) and reweigh.
- Include blank determinations and, where needed, defatting or carbonate removal steps.
- Reagents & apparatus: dilute sulfuric acid and potassium hydroxide solutions, filter aids, filter crucibles, cold-extraction/solvent devices and muffle furnace; water for analytical laboratory use (ISO 3696) is specified.
- Quality control: blank tests, repeatability and reproducibility data are reported (Annex A) and should be considered in method validation.
Applications
- Routine quality control of animal feed and feed ingredients.
- Analytical testing of cereals and pulses for formulation and nutritional labelling.
- Regulatory compliance testing where standardised, interlaboratory-comparable crude fibre results are required.
- Laboratories using either manual workflows or semi-automated digestion/filtration systems seeking an ISO-aligned method.
Related Standards
- ISO 6541 - alternative method for crude fibre ≤ 10 g/kg.
- ISO 3696 - water quality for analytical laboratory use.
- ISO 6498 - preparation of test samples of animal feedingstuffs.
- ISO 5725 - guidance on precision of methods (interlaboratory tests).
This concise summary highlights practical value: EN ISO 6865:2000 offers a reproducible, documented procedure suitable for feed testing laboratories that need consistent crude fibre measurements using intermediate filtration in manual or semi-automatic setups.
Frequently Asked Questions
SIST EN ISO 6865:2001 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)". This standard covers: Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg. NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
Cette norme internationale prescrit une méthode de détermination de la teneur en cellulose brute par filtration intermédiaire. Elle décrit un mode opératoire manuel et un mode opératoire semi-automatique. Cette méthode est applicable aux aliments des animaux ayant une teneur en cellulose brute supérieure à 10 g/kg. NOTE Pour des aliments pour animaux contenant de la cellulose brute inférieure ou égale à 10 g/kg, la méthode décrite dans l'ISO 6541 doit être utilisée. Cette Norme internationale est également applicable aux céréales et aux légumes.»
SIST EN ISO 6865:2001 is classified under the following ICS (International Classification for Standards) categories: 65.120 - Animal feeding stuffs. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 6865:2001 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Futtermittel - Bestimmung des Rohfasergehaltes - Verfahren mit Zwischenfiltration (ISO/FDIS 6865:2000)Aliments des animaux - Détermination de la teneur en cellulose brute - Méthode avec filtration intermédiaire (ISO/FDIS 6865:2000)Animal feeding stuffs - Determination of crude fibre content - Method with intermediate filtration (ISO 6865:2000)65.120KrmilaAnimal feeding stuffsICS:Ta slovenski standard je istoveten z:EN ISO 6865:2000SIST EN ISO 6865:2001en01-februar-2001SIST EN ISO 6865:2001SLOVENSKI
STANDARD
ReferencenumberISO6865:2000(E)©ISO2000INTERNATIONALSTANDARDISO6865Firstedition2000-10-15Animalfeedingstuffs—Determinationofcrudefibrecontent—MethodwithintermediatefiltrationAlimentsdesanimaux—Déterminationdelateneurencellulosebrute—MéthodeavecfiltrationintermédiaireSIST EN ISO 6865:2001
ISO6865:2000(E)PDFdisclaimerThisPDFfilemaycontainembeddedtypefaces.InaccordancewithAdobe'slicensingpolicy,thisfilemaybeprintedorviewedbutshallnotbeeditedunlessthetypefaceswhichareembeddedarelicensedtoandinstalledonthecomputerperformingtheediting.Indownloadingthisfile,partiesacceptthereintheresponsibilityofnotinfringingAdobe'slicensingpolicy.TheISOCentralSecretariatacceptsnoliabilityinthisarea.AdobeisatrademarkofAdobeSystemsIncorporated.DetailsofthesoftwareproductsusedtocreatethisPDFfilecanbefoundintheGeneralInforelativetothefile;thePDF-creationparameterswereoptimizedforprinting.EverycarehasbeentakentoensurethatthefileissuitableforusebyISOmemberbodies.Intheunlikelyeventthataproblemrelatingtoitisfound,pleaseinformtheCentralSecretariatattheaddressgivenbelow.©ISO2000Allrightsreserved.Unlessotherwisespecified,nopartofthispublicationmaybereproducedorutilizedinanyformorbyanymeans,electronicormechanical,includingphotocopyingandmicrofilm,withoutpermissioninwritingfromeitherISOattheaddressbeloworISO'smemberbodyinthecountryoftherequester.ISOcopyrightofficeCasepostale56CH-1211Geneva20Tel.+41227490111Fax+41227490947E-mailcopyright@iso.chWebwww.iso.chPrintedinSwitzerlandii©ISO2000–AllrightsreservedSIST EN ISO 6865:2001
ISO6865:2000(E)©ISO2000–AllrightsreservediiiForewordISO(theInternationalOrganizationforStandardization)isaworldwidefederationofnationalstandardsbodies(ISOmemberbodies).TheworkofpreparingInternationalStandardsisnormallycarriedoutthroughISOtechnicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeenestablishedhastherighttoberepresentedonthatcommittee.Internationalorganizations,governmentalandnon-governmental,inliaisonwithISO,alsotakepartinthework.ISOcollaboratescloselywiththeInternationalElectrotechnicalCommission(IEC)onallmattersofelectrotechnicalstandardization.InternationalStandardsaredraftedinaccordancewiththerulesgivenintheISO/IECDirectives,Part3.DraftInternationalStandardsadoptedbythetechnicalcommitteesarecirculatedtothememberbodiesforvoting.PublicationasanInternationalStandardrequiresapprovalbyatleast75%ofthememberbodiescastingavote.AttentionisdrawntothepossibilitythatsomeoftheelementsofthisInternationalStandardmaybethesubjectofpatentrights.ISOshallnotbeheldresponsibleforidentifyinganyorallsuchpatentrights.InternationalStandardISO6865waspreparedbyTechnicalCommitteeISO/TC34,Foodproducts,SubcommitteeSC10,Animalfeedingstuffs.AnnexAofthisInternationalStandardisforinformationonly.SIST EN ISO 6865:2001
INTERNATIONALSTANDARDISO6865:2000(E)©ISO2000–Allrightsreserved1Animalfeedingstuffs—Determinationofcrudefibrecontent—Methodwithintermediatefiltration1ScopeThisInternationalStandardspecifiesamethodwithintermediatefiltrationforthedeterminationofthecrudefibrecontent.Amanualprocedureandasemi-automaticprocedurearedescribed.Themethodisapplicabletoanimalfeedingstuffswithacrudefibrecontentgreaterthan10g/kg.NOTEForanimalfeedingstuffswithacrudefibrecontentequaltoorlessthan10g/kg,themethoddescribedinISO6541[7]maybeused.ThisInternationalStandardisalsoapplicabletocerealsandpulses.2NormativereferencesThefollowingnormativedocumentscontainprovisionswhich,throughreferenceinthistext,constituteprovisionsofthisInternationalStandard.Fordatedreferences,subsequentamendmentsto,orrevisionsof,anyofthesepublicationsdonotapply.However,partiestoagreementsbasedonthisInternationalStandardareencouragedtoinvestigatethepossibilityofapplyingthemostrecenteditionsofthenormativedocumentsindicatedbelow.Forundatedreferences,thelatesteditionofthenormativedocumentsreferredtoapplies.MembersofISOandIECmaintainregistersofcurrentlyvalidInternationalStandards.ISO3696:1987,Waterforanalyticallaboratoryuse—Specificationandtestmethods.ISO6498:1998,Animalfeedingstuffs—Preparationoftestsamples.3TermanddefinitionForthepurposesofthisInternationalStandard,thefollowingtermanddefinitionapply.3.1crudefibrecontentlossinmassresultingfromashingofthedriedresidueobtainedafteracidandalkalinedigestionofthesamplebytheproceduredescribedinthisInternationalStandard,dividedbythemassofthetestportionNOTEThecrudefibrecontentisexpressedingramsperkilogram.Itmayalsobeexpressedasamassfractioninpercent.4PrincipleThetestportionistreatedwithboilingdilutesulfuricacid.Theresidueisseparatedbyfiltration,washedandthentreatedwithboilingpotassiumhydroxidesolution.Theresidueisseparatedbyfiltration,washed,dried,weighedandthenashed.Thelossinmassresultingfromashingcorrespondstothemassofcrudefibreinthetestportion.SIST EN ISO 6865:2001
ISO6865:2000(E)2©ISO2000–Allrightsreserved5ReagentsandmaterialsUseonlyreagentsofrecognizedanalyticalgrade.5.1Water,complyingwithatleastgrade3inaccordancewithISO3696.5.2Hydrochloricacid,c(HCl)=0,5mol/l.5.3Sulfuricacid,c(H2SO4)=(0,130,005)mol/l.5.4Potassiumhydroxidesolution,c(KOH)=(0,230,005)mol/l.5.5Acetone.5.6Filteraid:seasand,orCelite®1)545,ormaterialofanequivalentquality.Beforeuse,treatseasandwithboilinghydrochloricacid[c(HCl)=4mol/l],washwithwateruntilfreefromacidandheatatatemperatureof(50025)°Cforatleast1h.Beforeuse,heatotherfilteraidsatatemperatureof(50025)°Cforatleast4h.5.7Antifoamagent,forinstancen-octanol.5.8Lightpetroleum,boilingrange40°Cto60°C.6ApparatusUsuallaboratoryapparatusand,inparticular,thefollowing.6.1Grindingdevice,capableofgrindingthesamplesothatitpassescompletelythroughasievewith1mmapertures.6.2Analyticalbalance,withaccuracyofatleast0,1mg.6.3Filtercrucibles,ofquartz,porcelainorhardglass,withfusedsinteredfilterplatewithaporesizeof40mto100m(porositygradeP100accordingtoISO4793:1980[1]).Beforeusingforthefirsttime,carefullyandgraduallyheatanewfiltercrucibletoatemperaturenotexceeding525°Candleaveforafewminutesat(50025)°C.Stainless-steelcrucibleswithstainless-steelsieveplateswithanaperturesizeof90mofidenticalperformancecharacteristicsmaybeusedasanalternative.6.4Porcelainsieveplates.6.5Incinerationdishes.6.6Beakersorconicalflasks,of500mlcapacity,providedwithasuitablecoolingdevice,forexampleacondensororadish.1)Celite®isthetradenameofacommerciallyavailableproduct.ThisinformationisgivenfortheconvenienceofusersofthisInternationalStandardanddoesnotconstituteanendorsementbyISOofthisproduct.Equivalentproductsmaybeusediftheycanbeshowntoleadtothesameresults.SIST EN ISO 6865:2001
ISO6865:2000(E)©ISO2000–Allrightsreserved36.7Dryingoven,electricallyheatedandventilated,capableofbeingmaintainedatatemperatureof(1302)°C.6.8Desiccator,containingbluesilicagelasdesiccant,providedwithaperforatedplate,preferablyofaluminiumorstainlesssteel,ofthickness2mmto3mm.6.9Mufflefurnace,electricallyheated,providedwithaircirculationandtemperaturecontrolcapableofmaintainingthetemperaturearoundthecruciblestothenearest25°Cattemperaturesof475°Cto525°C.Thereadingofthepyrometerofthemufflefurnacecannotalwaysbetrusted:deviationsmayoccur.Therefore,thetemperatureinthemufflefurnaceshouldbecheckedregularly.Dependingonthesizeandtypeofthemufflefurnace,temperaturesinthefurnacemaydifferfromoneplacetoanotherplace.Whentheovendoorisclosed,adequateairsupplyshouldbeguaranteed.Thevolumeflowrateofairshouldnotbesogreatthatmaterialiscarriedawayfromthecrucibles.6.10Cold-extractiondevice,providedwithasupportforthefiltercrucible(6.3);adischargepipewithataptothevacuumandliquidoutlet;andconnectingringsforconnectingthefiltercrucible(6.3).6.11Heatingdevice(formanualmethod),providedwithasuitablecoolingdevicecapableofmaintainingthevolumeconstantduringboiling.6.12Heatingdevice(forthesemi-automaticmethod)foracidandalkalinedigestion,providedwithasupportforthefiltercrucible(6.3);adischargepipewithataptothevacuumandliquidoutlet;aboilingcylinderofatleast270mlcapacity,witharefluxcondenser;connectingringsforconnectingtheheatingdevicetothefiltercrucible(6.3)andtheboilingcylinder;andoptionally,provisionforcompressedair.Beforeuse,preheattheapparatuswithboilingwaterfor5min.7SamplingSamplingisnotpartofthemethodspecifiedinthisInternationalStandard.ArecommendedsamplingmethodisgiveninISO6497[6].Itisimportantthatthelaboratoryreceiveasamplewhichistrulyrepresentativeandhasnotbeendamagedorchangedduringtransportorstorage.8PreparationoftestsamplePreparethetestsampleinaccordancewithISO6498.Usingthegrindingdevice(6.1),grindtheair-drylaboratorysamplesothatitpassescompletelythroughasievewith1mmapertures.Mixthoroughly.SIST EN ISO 6865:2001
ISO6865:2000(E)4©ISO2000–AllrightsreservedForthemanualmethod,proceedinaccordancewithclause9.Forthesemi-automaticmethod,proceedinaccordancewithclause10.9Procedureformanualmethod9.1TestportionWeighabout1gofthepreparedtestsample(clause8)tothenearest0,1mg(m1).Ifthefatcontentofthesampleexceeds100g/kg,orifthesamplecontainsfatswhichcannotbeextracteddirectlywithlightpetroleum(5.8),transferthesampletoacrucible(6.3)andproceedinaccordancewith9.2.Ifthefatcontentofthesampledoesnotexceed100g/kg,transferthesampletoabeaker(6.6)andproceedinaccordancewith9.3ifthecarbonatecontent,expressedascalciumcarbonate,exceeds50g/kg.Ifthisisnotthecase,proceedinaccordancewith9.4.9.2PreliminarydefattingInthecold-extractiondevice(6.10),defatthesamplethreetimesundervacuumwith30mloflightpetroleum(5.8)eachtime.Drytheresiduebysuctionaftereachwashing.Transfertheresiduetoabeaker(6.6).Ifthecarbonatecontent,expressedascalciumcarbonate,exceeds50g/kg,proceedinaccordancewith9.3.Ifnot,proceedinaccordancewith9.4.9.3RemovalofcarbonatePour100mlofhydrochloricacid(5.2)overthesample.Stircontinuouslyfor5min.Carefullypourthemixtureintoafiltercrucible,thebottomofwhichiscoveredwithathinlayeroffilteraid(5.6).Decanttwicewith100mlofwatereachtime.Takecaresothataslittlematerialaspossibleendsuponthefilter.Transferthecontentsofthecrucibletotheoriginalbeakerandproceedinaccordancewith9.4.9.4AciddigestionPour150mlofsulfuricacid(5.3)overthesample.Bringtotheboilasquicklyaspossibleandcontinuesteadyboilingfor(301)min.Atthebeginningofboiling,swirlafewtimes.Iffoamingoccurs,addafewdropsofantifoamagent(5.7).Duringboiling,maintainaconstantvolumebyusingasuitablecoolingdevice(see6.6and6.11).9.5FirstfiltrationInthefiltercrucible(6.3)applyalayeroffilteraid
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