SIST EN 18079:2025

SLOVENSKI STANDARD
01-september-2025
Lesne plošče - Določanje količine prostega melamina z ekstrakcijo in s tekočinsko
kromatografijo visoke ločljivosti (HPLC) z ultravijolično detekcijo
Wood-based panels - Determination of free melamine applying extraction and high-
performance liquid chromatography (HPLC) with ultraviolet detection
Holzwerkstoffe - Bestimmung von freiem Melamin durch Extraktion und
Hochleistungsflüssigkeitschromatographie (HPLC) mit Ultraviolett-Detektion
Panneaux à base de bois - Détermination de la mélamine libre par extraction et
chromatographie liquide à haute performance (HPLC) avec détection ultraviolette
Ta slovenski standard je istoveten z: EN 18079:2025
ICS:
79.060.01 Lesne plošče na splošno Wood-based panels in
general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN 18079
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2025
EUROPÄISCHE NORM
ICS 79.060.01
English Version
Wood-based panels - Determination of free melamine
applying extraction and high-performance liquid
chromatography (HPLC) with ultraviolet detection
Panneaux à base de bois - Détermination de la Holzwerkstoffe - Bestimmung von freiem Melamin
mélamine libre par extraction et chromatographie durch Extraktion und
liquide à haute performance (HPLC) avec détection Hochleistungsflüssigkeitschromatographie (HPLC) mit
ultraviolette Ultraviolett-Detektion
This European Standard was approved by CEN on 23 June 2025.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 18079:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents . 5
6 Apparatus . 6
7 Sampling . 6
7.1 General. 6
7.2 Sample preparation . 6
7.3 Extraction . 7
7.3.1 General procedure solid sample material . 7
7.3.2 General procedure liquid sample material . 7
7.3.3 Extract dilution for low melamine content . 7
7.3.4 Extract dilution for high melamine content . 7
8 Procedure . 7
8.1 HPLC analysis . 7
8.1.1 General. 7
8.1.2 Eluent preparation . 7
8.1.3 HPLC parameters . 8
8.1.4 Calibration . 8
8.1.5 Measurement and calculation . 8
8.1.6 Detection limits . 9
8.1.7 Quality assurance . 9
8.1.8 Maintenance . 9
9 Test report . 10
Annex A (informative) Example of HPLC parameters . 11
Annex B (informative) Example for an estimation of detection limits . 13
Bibliography . 14

European foreword
This document (EN 18079:2025) has been prepared by Technical Committee CEN/TC 112 “Wood-based
panels”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2026, and conflicting national standards shall
be withdrawn at the latest by January 2026.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a standardization request addressed to CEN by the European
Commission. The Standing Committee of the EFTA States subsequently approves these requests for its
Member States.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organisations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia,
Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland,
Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of North
Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the United
Kingdom.
Introduction
Melamine is part of adhesives (melamine-urea-formaldehyde resins, MUF) and impregnation resins
(melamine-formaldehyde-resins, MF) and is therefore present in wood-based products as well as
products with a decorative wear layer. Fully cured products contain mainly chemically bound melamine,
which makes the presence of free melamine unlikely.
Melamine (2,4,6-Triamino-1,3,5-triazine) is a heterocyclic, aromatic substance (CAS 108-78-1), see
Figure 1. It is a white solid at room temperature with a melting point of 280 °C. It decomposes above this
temperature. Melamine is poorly soluble in cold water. In hot water, melamine has a significantly higher
solubility. Dimethyl sulfoxide (DMSO) is an inert solvent and capable for melamine quantification.

Figure 1 — Melamine structure (CAS: 108-78-1), molar mass 126,12 g/mol
1 Scope
This document specifies the determination of free melamine in coated and uncoated wood-based-panels.
NOTE 1 It is also applicable to other wood-based products, to other solid products e.g. impregnates or decorative
paper and to liquid materials.
The determination of melamine is performed by extraction using dimethyl sulfoxide (DMSO) and
subsequent high-performance liquid chromatography (HPLC) analysis and ultraviolet (UV) detection.
NOTE 2 For determination of melamine in foodstuff, EN 16858 is applicable. For determination of melamine in
animal feeding stuffs, EN 17212 is applicable. For determination of melamine in textiles, EN ISO 1833-26 is
applicable.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
EN 322, Wood-based panels — Determination of moisture content
EN 326-1, Wood-based panels — Sampling, cutting and inspection — Part 1: Sampling and cutting of test
pieces and expression of test results
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp
— IEC Electropedia: available at https://www.electropedia.org/
4 Principle
A sample of a wood-based panel product is milled and extracted with dimethyl sulfoxide (DMSO). After
adding acetonitrile, the content of free melamine in the solution is determined by high-performance
liquid chromatography (HPLC) analysis and UV detection.
5 Reagents
5.1 Dimethyl sulfoxide (DMSO) of analytical grade.
5.2 Water (deionized).
5.3 Acetonitrile (AcN) of analytical grade.
5.4 Ammonium formate of analytical grade.
5.5 Melamine of analytical grade.
6 Apparatus
6.1 Cryomill (recommended).
6.2 Drying oven for determination of solid content.
6.3 HPLC device offering column oven and diode array detector (DAD).
6.4 Laboratory shaker.
6.5 Screw cap bottles, 20 ml.
6.6 HPLC vials, 2 ml.
6.7 Disposable filter (PTFE, 0,2 µm), disposable syringe.
7 Sampling
7.1 General
A representative sample of the wood-based panel product to be tested is collected. From this sample, a
set of 5 test pieces shall be cut and used for subsequent procedures. The tests pieces shall be cut according
to EN 326-1. The sample and the test pieces shall be stored in dust-free and moisture-impermeable
containers or bags (aluminium or PE).
NOTE Other solid materials (wood-based products, decoration paper, impregnates, dust, laminate) and liquids
can also be sampled and investigated.
The sample preparation is adapted to the content of free melamine expected in the sample material. High
contents of free melamine are expected in resins and not fully cured samples only. The extracts will be
diluted to preserve the HPLC column. DMSO dissolves not only melamine but also resin components
present in not fully cured samples. These components shall be separated before HPLC analysis. Therefore,
acetonitrile is added leading to precipitation of these resin components.
Free melamine soluble in DMSO remains dissolved and is completely available to subsequent analysis.
7.2 Sample preparation
Samples shall be analysed as they are and shall not be manipulated (e.g. by drying) to avoid any further
reactions.
Samples shall be representative of the material to be tested. In the case of panels, the samples and test
pieces shall be taken in the entire thickness of the board.
The solid sample material is milled to particle size preferably below 1 mm. To avoid excessive heat,
cryomilling is recommended. A representative sample amount requires at least 10 g of the test material.
The milled sample material shall be stored in dust-protected bags at room temperature.
NOTE Liquid samples are stored as specified in
...