SIST EN ISO 16187:2025

SLOVENSKI STANDARD
01-maj-2025
Obutev in deli obutve - Preskusna metoda za ugotavljanje protibakterijskega
delovanja (ISO 16187:2025)
Footwear and footwear components - Test method to assess antibacterial activity (ISO
16187:2025)
Schuhe und Schuhbestandteile - Prüfverfahren zur Bestimmung der antibakteriellen
Wirkung (ISO 16187:2025)
Chaussure et composants de chaussure - Méthode d'essai pour évaluer l'activité
antibactérienne (ISO 16187:2025)
Ta slovenski standard je istoveten z: EN ISO 16187:2025
ICS:
61.060 Obuvala Footwear
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 16187
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2025
EUROPÄISCHE NORM
ICS 61.060 Supersedes EN ISO 16187:2013
English Version
Footwear and footwear components - Test method to
assess antibacterial activity (ISO 16187:2025)
Chaussure et composants de chaussure - Méthode Schuhe und Schuhbestandteile - Prüfverfahren zur
d'essai pour évaluer l'activité antibactérienne (ISO Bestimmung der antibakteriellen Wirkung (ISO
16187:2025) 16187:2025)
This European Standard was approved by CEN on 4 March 2025.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16187:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 16187:2025) has been prepared by Technical Committee ISO/TC 216
"Footwear" in collaboration with Technical Committee CEN/TC 309 “Footwear” the secretariat of which
is held by UNE.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2025, and conflicting national standards
shall be withdrawn at the latest by September 2025.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 16187:2013.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 16187:2025 has been approved by CEN as EN ISO 16187:2025 without any modification.

International
Standard
ISO 16187
Second edition
Footwear and footwear
2025-02
components — Test method to
assess antibacterial activity
Chaussure et composants de chaussure — Méthode d'essai pour
évaluer l'activité antibactérienne
Reference number
ISO 16187:2025(en) © ISO 2025
ISO 16187:2025(en)
© ISO 2025
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
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Published in Switzerland
ii
ISO 16187:2025(en)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Safety . 2
6 Apparatus . 2
7 Reagents and culture medium . 2
7.1 General .2
7.2 Water .3
7.3 Nutrient broth (NB) .3
7.3.1 Composition .3
7.3.2 Preparation .3
7.4 Nutrient agar (NA) .3
7.4.1 Composition .3
7.4.2 Preparation .3
7.5 Tryptic soy broth (TSB) .4
7.5.1 Composition .4
7.5.2 Preparation .4
7.6 Tryptone soy agar (TSA) .4
7.6.1 Composition .4
7.6.2 Preparation .4
7.7 Soybean casein digest broth with lecithin and polyoxyethylene medium (SCDLP) .4
7.7.1 Composition .4
7.7.2 Preparation .5
7.8 Sodium chloride solution (physiological saline).5
7.8.1 Composition .5
7.8.2 Preparation .5
8 Test microorganisms . 5
8.1 Test strains.5
8.2 Storage of strains .5
9 Preparation of test inoculums . 6
10 Preparation of test samples . 6
10.1 General .6
10.2 Test specimen .6
10.3 Pre-treatment of the test specimen .6
11 Test procedure . 6
12 Expression of results . 7
12.1 Calculation of the number of viable bacteria .7
12.2 Judgement of test effectiveness .7
12.3 Calculation of antibacterial activity ratio .8
13 Test report .
...