SIST ISO 11027:2026
(Main)Pepper and pepper oleoresins — Determination of piperine content — Method using high-performance liquid chromatography (ISO 11027:2026)
- Abstract
This document specifies a method for the determination of piperine content of peppers (Piper nigrum Linnaeus), whole or ground, as well as their extracts (Oleoresins) by high performance liquid chromatography.
This method also enables the separation and, if necessary, the determination of the other alkaloids of pepper (isochavicine, isopiperine and piperittin).
- Status
- Published
- Publication Date
- 05-Oct-2026
- Technical Committee
- KŽP - Agricultural food products
- Current Stage
- 6060 - National Implementation/Publication (Adopted Project)
- Start Date
- 06-Oct-2026
- Due Date
- 11-Dec-2026
- Completion Date
- 06-Oct-2026
Relations
- Effective Date
- 01-Nov-2026
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Frequently Asked Questions
SIST ISO 11027:2026 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Pepper and pepper oleoresins — Determination of piperine content — Method using high-performance liquid chromatography (ISO 11027:2026)". This standard covers: This document specifies a method for the determination of piperine content of peppers (Piper nigrum Linnaeus), whole or ground, as well as their extracts (Oleoresins) by high performance liquid chromatography. This method also enables the separation and, if necessary, the determination of the other alkaloids of pepper (isochavicine, isopiperine and piperittin).
This document specifies a method for the determination of piperine content of peppers (Piper nigrum Linnaeus), whole or ground, as well as their extracts (Oleoresins) by high performance liquid chromatography. This method also enables the separation and, if necessary, the determination of the other alkaloids of pepper (isochavicine, isopiperine and piperittin).
SIST ISO 11027:2026 is classified under the following ICS (International Classification for Standards) categories: 67.220.10 - Spices and condiments. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST ISO 11027:2026 has the following relationships with other standards: It is inter standard links to SIST ISO 11027:1997. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
SIST ISO 11027:2026 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
SLOVENSKI STANDARD
01-november-2026
Nadomešča:
SIST ISO 11027:1997
Poper in oleorezini popra - Določanje piperina - Metoda s tekočinsko
kromatografijo visoke ločljivosti (ISO 11027:2026)
Pepper and pepper oleoresins — Determination of piperine content — Method using
high-performance liquid chromatography (ISO 11027:2026)
Poivres, oléorésines de poivres — Détermination de la teneur en pipérine — Méthode
par chromatographie en phase liquide à haute performance (ISO 11027:2026)
Ta slovenski standard je istoveten z: ISO 11027:2026
ICS:
67.220.10 Začimbe Spices and condiments
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
International
Standard
ISO 11027
Second edition
Pepper and pepper oleoresins —
2026-03
Determination of piperine content
— Method using high-performance
liquid chromatography
Poivres, oléorésines de poivres — Détermination de la teneur en
pipérine — Méthode par chromatographie en phase liquide à
haute performance
Reference number
© ISO 2026
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
4.1 Ground pepper .1
4.2 Whole pepper .1
4.3 Oleoresins of pepper .1
5 Reagents . 2
6 Apparatus . 2
7 Calibration method . 3
7.1 Reference solution .3
7.2 Calibration curve .3
7.3 Calculation of the response factor, K .3
8 Sampling . 3
9 Preparation of test sample . 3
9.1 Peppers in powder form .4
9.2 Whole peppers .4
9.3 Oleoresins of peppers .4
10 Procedure . 4
10.1 Test portion .4
10.1.1 Peppers, whole or ground .4
10.1.2 Oleoresins of pepper .4
10.2 Determination .4
11 Calculation . 5
11.1 Peppers, whole or ground .5
11.2 Oleoresins of pepper .5
12 Repeatability . 5
13 Test report . 5
Annex A (informative) Typical chromatograms. 6
Bibliography . 7
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO’s adherence to
...
International
Standard
ISO 11027
Second edition
Pepper and pepper oleoresins —
2026-03
Determination of piperine content
— Method using high-performance
liquid chromatography
Poivres, oléorésines de poivres — Détermination de la teneur en
pipérine — Méthode par chromatographie en phase liquide à
haute performance
Reference number
© ISO 2026
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
4.1 Ground pepper .1
4.2 Whole pepper .1
4.3 Oleoresins of pepper .1
5 Reagents . 2
6 Apparatus . 2
7 Calibration method . 3
7.1 Reference solution .3
7.2 Calibration curve .3
7.3 Calculation of the response factor, K .3
8 Sampling . 3
9 Preparation of test sample . 3
9.1 Peppers in powder form .4
9.2 Whole peppers .4
9.3 Oleoresins of peppers .4
10 Procedure . 4
10.1 Test portion .4
10.1.1 Peppers, whole or ground .4
10.1.2 Oleoresins of pepper .4
10.2 Determination .4
11 Calculation . 5
11.1 Peppers, whole or ground .5
11.2 Oleoresins of pepper .5
12 Repeatability . 5
13 Test report . 5
Annex A (informative) Typical chromatograms. 6
Bibliography . 7
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO’s adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 34, Food products, Committee Subcommittee
SC 7, Spices, culinary herbs and condiments.
This second edition cancels and replaces the first edition (ISO 11027:1993), which has been technically
revised.
The main changes are as follows:
— the references have been updated and usage of the term s
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