SIST EN ISO 6867:2001

Overview

EN ISO 6867:2000 - titled "Animal feeding stuffs - Determination of vitamin E content - Method using high-performance liquid chromatography (ISO 6867:2000)" - specifies an HPLC-based method for measuring vitamin E (DL-α‑tocopherol) in animal feedingstuffs and pet foods. Published by CEN as a harmonized adoption of the ISO method, this standard defines a laboratory procedure to obtain reliable, comparable results for vitamin E content across feed matrices.

Key Topics

• Analyte: DL-α‑tocopherol (vitamin E).
• Analytical technique: High-performance liquid chromatography (HPLC) as the primary quantitative method.
• Sample scope: Animal feeds, feed materials, premixes and pet foods (various matrices common to feed analysis).
• General procedure elements: sample preparation and extraction, chromatographic separation and detection, quantification using calibration with reference standards, and result expression for reporting.
• Quality considerations: Emphasis on reproducibility and traceability of results suitable for quality control and regulatory purposes.
• Standards context: Adopted as EN ISO to align national and European feed testing practices with the ISO method.

Note: EN ISO 6867:2000 defines the method framework and performance expectations; laboratories should follow good analytical practice (method validation, calibration, internal quality control) when implementing the procedure.

Applications

• Feed manufacturers: Verify vitamin E content in premixes and compounded feeds for nutritional specification and labelling.
• Pet food producers: Ensure ingredient and finished-product compliance with declared vitamin E levels.
• Quality control laboratories: Routine testing for batch release, stability studies, and supplier verification.
• Regulatory and certification bodies: Enforcement of feed composition regulations and verification of compliance with nutritional standards.
• Research laboratories: Nutritional studies, formulation development, and stability testing involving vitamin E in animal feeds.

Benefits

• Provides a standardized, reproducible analytical route for vitamin E determination.
• Supports compliance, traceability, and comparability of results across laboratories and markets.
• Useful for both routine QC and research-grade analyses.

Related Standards

• Other ISO and EN methods addressing feed composition, vitamin analyses, and laboratory quality systems may be relevant when implementing EN ISO 6867:2000 (e.g., standards for feed sampling, general HPLC practices, and method validation).

Keywords: EN ISO 6867:2000, vitamin E determination, DL-α‑tocopherol, animal feedingstuffs, pet food analysis, HPLC method, feed quality control, CEN standard.