SIST EN ISO 6320:2017
(Main)Animal and vegetable fats and oils - Determination of refractive index (ISO 6320:2017)
Animal and vegetable fats and oils - Determination of refractive index (ISO 6320:2017)
ISO 6320:2017 specifies a method for the determination of the refractive index of animal and vegetable fats and oils.
Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
Tierische und pflanzliche Fette und Öle - Bestimmung des Brechungsindex (ISO 6320:2017)
Dieses Dokument legt ein Verfahren zur Bestimmung des Brechungsindex von tierischen und pflanzlichen Fetten und Ölen fest.
Milch und Milcherzeugnisse (oder Fett aus Milch und Milcherzeugnissen) sind vom Anwendungsbereich dieses Dokuments ausgenommen.
Corps gras d'origines animale et végétale - Détermination de l'indice de réfraction (ISO 6320:2017)
ISO 6320:2017 spécifie une méthode de détermination de l'indice de réfraction des corps gras d'origines animale et végétale.
Le lait et les produits laitiers (ou les corps gras issus du lait et des produits laitiers) ne relèvent pas du domaine d'application du présent document.
Rastlinske in živalske maščobe in olja - Določevanje lomnega koeficienta (ISO 6320:2017)
Ta dokument določa metodo za določevanje lomnega koeficienta živalskih in rastlinskih masti in olj.
Mleko in mlečni izdelki (ali maščoba iz mleka in mlečnih izdelkov) niso zajeti v tem dokumentu.
General Information
Relations
Overview
EN ISO 6320:2017 (ISO 6320:2017) specifies a standardized method for the determination of refractive index of animal and vegetable fats and oils. The procedure covers sample preparation, instrument calibration, measurement temperatures and calculation rules for reporting refractive index values. Milk and milk‑derived fats are excluded from the scope.
Key topics and technical requirements
- Scope: Measurement of refractive index for animal and vegetable fats and oils (excludes milk fats).
- Instruments: Abbé‑type refractometers or modern digital refractometers; instrument accuracy to ±0.0001 over n = 1.300–1.700.
- Light source: Sodium vapour lamp (or white light with achromatic compensation).
- Temperature control: Prism temperature must be maintained and monitored; water baths with thermostatic control (±0.1 °C) are used for temperature stability.
- Calibration: Verify calibration using a glass plate of known refractive index or ethyl laurate of known refractive index.
- Sample prep: Follow ISO 661 for preparation; determine refractive index on dried and filtered fats and oils. Solid samples must be fully melted and temperature‑stabilized before measurement.
- Measurement temperatures: Typical reference temperatures are 20 °C, 40 °C, 50 °C, 60 °C, 80 °C or higher, chosen depending on melting behavior of the sample.
- Cleaning reagents: Solvents such as hexane, light petroleum, acetone or toluene are recommended for prism cleaning.
- Procedure & calculation:
- Take three independent readings and report the arithmetic mean to four decimal places.
- If measurement temperature differs from the reference by < 3 °C, apply the standard temperature correction factor F (e.g., 0.00035 at 20 °C; 0.00036 at 40–60 °C; 0.00037 at 80 °C+).
- If the temperature difference is ≥ 3 °C, repeat the measurement.
- Precision: The standard includes provisions for repeatability, reproducibility and interlaboratory testing (see ISO 5725 series).
Practical applications and users
- Quality control laboratories in edible oil, fats and oleochemical industries use EN ISO 6320:2017 for product specification and batch verification.
- Food processors and refiners apply refractive index testing for identity, purity checks and process control.
- Regulatory bodies and testing laboratories use this method for standardized reporting and interlaboratory comparisons.
- R&D teams employ refractive index data to characterize lipid blends, detect adulteration and support product development.
Related standards
- ISO 661 - Preparation of test sample (fats and oils)
- ISO 5555 - Recommended sampling for oils and fats (sampling not in scope)
- ISO 5725‑1 / ISO 5725‑2 - Accuracy, repeatability and reproducibility of measurement methods
Using EN ISO 6320:2017 ensures consistent, comparable refractive index results across laboratories, supporting product quality, compliance and reliable analytical reporting for animal and vegetable fats and oils.
Standards Content (Sample)
SLOVENSKI STANDARD
01-maj-2017
1DGRPHãþD
SIST EN ISO 6320:2001
SIST EN ISO 6320:2001/AC:2007
5DVWOLQVNHLQåLYDOVNHPDãþREHLQROMD'RORþHYDQMHORPQHJDNRHILFLHQWD ,62
Animal and vegetable fats and oils - Determination of refractive index (ISO 6320:2017)
Tierische und pflanzliche Fette und Öle - Bestimmung des Brechungsindex (ISO
6320:2017)
Corps gras d'origines animale et végétale - Détermination de l'indice de réfraction (ISO
6320:2017)
Ta slovenski standard je istoveten z: EN ISO 6320:2017
ICS:
67.200.10 5DVWOLQVNHLQåLYDOVNH Animal and vegetable fats
PDãþREHLQROMD and oils
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 6320
EUROPEAN STANDARD
NORME EUROPÉENNE
March 2017
EUROPÄISCHE NORM
ICS 67.200.10 Supersedes EN ISO 6320:2000
English Version
Animal and vegetable fats and oils - Determination of
refractive index (ISO 6320:2017)
Corps gras d'origines animale et végétale - Tierische und pflanzliche Fette und Öle - Bestimmung
Détermination de l'indice de réfraction (ISO des Brechungsindex (ISO 6320:2017)
6320:2017)
This European Standard was approved by CEN on 9 March 2017.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6320:2017 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 6320:2017) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats
and oils and their by-products - Methods of sampling and analysis” the secretariat of which is held by
AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by September 2017, and conflicting national standards
shall be withdrawn at the latest by September 2017.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent
rights.
This document supersedes EN ISO 6320:2000.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 6320:2017 has been approved by CEN as EN ISO 6320:2017 without any modification.
INTERNATIONAL ISO
STANDARD 6320
Fifth edition
2017-02
Animal and vegetable fats and oils —
Determination of refractive index
Corps gras d’origines animale et végétale — Détermination de l’indice
de réfraction
Reference number
ISO 6320:2017(E)
©
ISO 2017
ISO 6320:2017(E)
© ISO 2017, Published in Switzerland
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
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ii © ISO 2017 – All rights reserved
ISO 6320:2017(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative reference . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 2
7 Sampling . 2
8 Preparation of test sample . 2
9 Procedure. 2
9.1 Calibration of the instrument . 3
9.2 Determination . 3
10 Calculation . 3
11 Precision . 4
11.1 Interlaboratory test. 4
11.2 Repeatability . 4
11.3 Reproducibility . 4
12 Test report . 4
Annex A (normative) Results of an interlaboratory test. 5
Bibliography . 6
ISO 6320:2017(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (s
...
Frequently Asked Questions
SIST EN ISO 6320:2017 is a standard published by the Slovenian Institute for Standardization (SIST). Its full title is "Animal and vegetable fats and oils - Determination of refractive index (ISO 6320:2017)". This standard covers: ISO 6320:2017 specifies a method for the determination of the refractive index of animal and vegetable fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
ISO 6320:2017 specifies a method for the determination of the refractive index of animal and vegetable fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.
SIST EN ISO 6320:2017 is classified under the following ICS (International Classification for Standards) categories: 67.200.10 - Animal and vegetable fats and oils. The ICS classification helps identify the subject area and facilitates finding related standards.
SIST EN ISO 6320:2017 has the following relationships with other standards: It is inter standard links to SIST EN ISO 6320:2001/AC:2007, SIST EN ISO 6320:2001. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase SIST EN ISO 6320:2017 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of SIST standards.
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