SIST EN ISO 835:2007

SLOVENSKI STANDARD
SIST EN ISO 835:2007
01-september-2007
Laboratorijska steklovina - Graduirane pipete (ISO 835:2007)
Laboratory glassware - Graduated pipettes (ISO 835:2007)
Laborgeräte aus Glas - Messpipetten (ISO 835:2007)
Verrerie de laboratoire - Pipettes graduées (ISO 835:2007)
Ta slovenski standard je istoveten z: EN ISO 835:2007
ICS:
71.040.20 Laboratorijska posoda in Laboratory ware and related
aparati apparatus
SIST EN ISO 835:2007 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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EUROPEAN STANDARD
EN ISO 835
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2007
ICS 17.060

English Version
Laboratory glassware - Graduated pipettes (ISO 835:2007)
Verrerie de laboratoire - Pipettes graduées (ISO 835:2007) Laborgeräte aus Glas - Messpipetten (ISO 835:2007)
This European Standard was approved by CEN on 13 March 2007.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 835:2007: E
worldwide for CEN national Members.

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EN ISO 835:2007 (E)





Foreword


This document (EN ISO 835:2007) has been prepared by Technical Committee ISO/TC 48
"Laboratory glassware and related apparatus" in collaboration with Technical Committee
CEN/TC 332 "Laboratory equipment", the secretariat of which is held by DIN.

This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by October 2007, and conflicting national
standards shall be withdrawn at the latest by October 2007.

According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United
Kingdom.


Endorsement notice

The text of ISO 835:2007 has been approved by CEN as EN ISO 835:2007 without any
modifications.


2

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INTERNATIONAL ISO
STANDARD 835
First edition
2007-04-01


Laboratory glassware — Graduated
pipettes
Verrerie de laboratoire — Pipettes graduées




Reference number
ISO 835:2007(E)
©
ISO 2007

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ISO 835:2007(E)
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ii © ISO 2007 – All rights reserved

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ISO 835:2007(E)
Contents Page
Foreword. iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 1
4 Basis of adjustment. 2
4.1 Unit of volume. 2
4.2 Delivery volume . 2
4.3 Reference temperature. 2
5 Types and classes of accuracy. 2
5.1 Classes of accuracy . 2
5.2 Types of pipettes . 2
6 Maximum permissible errors. 3
7 Construction. 4
7.1 Material . 4
7.2 Dimensions. 4
7.3 Top of pipette . 4
7.4 Delivery jet. 4
7.5 Delivery time. 5
7.6 Waiting time. 5
8 Graduation, figuring and patterns. 5
8.1 Graduation patterns. 5
8.2 Position of graduation lines . 5
8.3 Figuring of graduation lines . 5
9 Setting of the meniscus . 6
10 Marking . 6
11 Visibility of graduation lines, figures and inscriptions. 7
12 Colour coding. 7
Annex A (normative) Definition of capacities and delivery times. 8
Bibliography . 12

© ISO 2007 – All rights reserved iii

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ISO 835:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 835 was prepared by Technical Committee ISO/TC 48, Laboratory equipment, Subcommittee SC 6,
Laboratory and volumetric ware.
This first edition of ISO 835 cancels and replaces ISO 835-1:1981, ISO 835-2:1981, ISO 835-3:1981 and
ISO 835-4:1981, which have been technically revised and combined into one document.

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INTERNATIONAL STANDARD ISO 835:2007(E)

Laboratory glassware — Graduated pipettes
1 Scope
This International Standard specifies metrological and constructional requirements for graduated pipettes,
adequate for general laboratory purposes.
The details specified are in conformity with the principles of design and construction of volumetric glassware
given in ISO 384.
NOTE For one-mark pipettes, see ISO 648. For piston-operated pipettes, see ISO 8655-2.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 384:1978, Laboratory glassware — Principles of design and construction of volumetric glassware
ISO 719, Glass — Hydrolytic resistance of glass grains at 98 °C — Method of test and classification
ISO 1769, Laboratory glassware — Pipettes — Colour coding
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
delivery volume
volume of liquid discharged from the pipette
NOTE Due to retention of liquid on the inner surface of the pipette, the volume of liquid delivered is not identical with
the volume of liquid contained by the pipette.
3.2
delivery time
time required for the pipette to deliver its nominal volume
3.3
waiting time
time to be observed after apparent completion of the liquid delivery of the pipette and before the final reading
of the delivered volume is taken
NOTE A waiting time applies for graduated pipettes of Class AS (see 5.1 and 7.6).
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ISO 835:2007(E)
4 Basis of adjustment
4.1 Unit of volume
3
The unit of volume shall be the millilitre (ml) which is equivalent to the cubic centimetre (cm ).
4.2 Delivery volume
Graduated pipettes shall be adjusted with water according to ISO 3696, grade 3, to deliver their specified
volume (Ex). The delivery should be under gravity and unrestricted. For details, see 7.5.
4.3 Reference temperature
The standard reference temperature, i.e. the temperature at which the pipette is intended to deliver its volume,
shall be 20 °C.
When the pipette is required for use in a country which has adopted a standard reference temperature of
27 °C (the alternative recommended in ISO 384 for tropical use), this figure shall be substituted for 20 °C.
5 Types and classes of accuracy
5.1 Classes of accuracy
Two classes of accuracy are specified:
⎯ Classes A and AS for the higher grade;
⎯ Class B for the lower grade.
The maximum permissible errors for both classes are specified in Table 1.
The pipettes shall be adjusted as follows:
⎯ Class A: without waiting time;
⎯ Class AS: with a specified waiting time of 5 s (see 7.6);
⎯ Class B: without waiting time.
5.2 Types of pipettes
5.2.1 Type 1: Partial delivery
Graduated pipettes adjusted for delivery of a liquid from zero line at the top to any graduation line; nominal
capacity shall be represented by the lowest graduation line.
5.2.2 Type 2: Total delivery
Graduated pipettes adjusted for delivery of a liquid from any graduation line down to the jet; nominal capacity
shall be represented by the highest graduation line.
2 © ISO 2007 – All rights reserved

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ISO 835:2007(E)
5.2.3 Type 3: Total delivery
Graduated pipettes adjusted for delivery of a liquid from zero line at the top to any graduation line; nominal
capacity shall be obtained by delivery down to the jet.
5.2.4 Type 4: Blow-out
Graduated pipettes for total delivery (see Type 3) where the last drop of liquid in the jet is expelled by blowing.
Blow-out pipettes shall be adjusted to accuracy Class B.
6 Maximum permissible errors
Maximum permissible errors in the delivered volume shall not exceed the limits specified in Table 1. These
limits represent the maximum permitted error at any point and also the maximum permissible difference
between the errors at any two points.
Table 1 — Capacities, subdivisions and maximum permissible errors
Nominal capacity Smallest scale Maximum permissible error
division
 Classes A and AS Class B
ml ml ± ml ± ml
0,1 0,01 0,006 0,01
0,2 0,01 0,006 0,01
0,5 0,01 0,006 0,01
1 0,01 0,007 0,01
1 0,10 0,007 0,01
2 0,02 0,010 0,02
2 0,10 0,010 0,02
5 0,05 0,030 0,05
5 0,10 0,030 0,05
10 0,1 0,05 0,1
20 0,1 0,1 0,2
a
25 0,1 0,1 0,2
25 0,2 0,1 0,2
a
Length 450 mm, see Table 2.

If graduated pipettes are required of capacities and/or subdivisions other than those listed in Table 1, they
shall conform to the essential requirements of this International Standard.
The relationships between maximum permissible error and capacity, as well as between maximum
permissible error and meniscus diameter as given in ISO 384:1978, Annex A and Annex B, shall be observed.
© ISO 2007 – All rights reserved 3

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