oSIST prEN 49-2:2025

SLOVENSKI STANDARD
01-december-2025
Biocidni proizvodi za zaščito lesa - Ugotavljanje učinkovitosti preventivne zaščite
proti navadnemu trdoglavcu Anobium punctatum (De Geer) glede na število
odloženih jajčec in preživelih ličink - 2. del: Postopek z globinsko impregnacijo
lesa (laboratorijska metoda)
Wood preservatives - Determination of the protective effectiveness against Anobium
punctatum (De Geer) by egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenüber Anobium
punctatum (De Geer) durch Beobachten der Eiablage und des Überlebens von Larven -
Teil 2: Anwendung durch Volltränkung (Laboratoriumsverfahren)
Produits de préservation du bois - Détermination de l'efficacité protectrice vis-à-vis
d'Anobium punctatum (De Geer) par l'observation de la ponte et du taux de survie des
larves - Partie 2 : Application par imprégnation (Méthode de laboratoire)
Ta slovenski standard je istoveten z: prEN 49-2
ICS:
71.100.50 Kemikalije za zaščito lesa Wood-protecting chemicals
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
EUROPEAN STANDARD
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2025
ICS 71.100.50 Will supersede EN 49-2:2015
English Version
Wood preservatives - Determination of the protective
effectiveness against Anobium punctatum (De Geer) by
egg-laying and larval survival - Part 2: Application by
impregnation (Laboratory method)
Produits de préservation du bois - Détermination de Holzschutzmittel - Bestimmung der vorbeugenden
l'efficacité protectrice vis à vis de Anobium punctatum Wirkung gegenüber Anobium punctatum (De Geer)
(De Geer) par l'observation de la ponte et de la survie durch Beobachten der Eiablage und des Überlebens
des larves - Partie 2 : Application par imprégnation von Larven - Teil 2: Anwendung durch Volltränkung
(Méthode de laboratoire) (Laboratoriumsverfahren)
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 38.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATIO N

EUROPÄISCHES KOMITEE FÜR NORMUN G

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2025 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 49-2:2025 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative reference . 5
3 Terms and definitions . 5
4 Principle . 5
5 Test materials . 6
5.1 Biological material . 6
5.2 Products and reagents . 6
5.3 Apparatus . 7
6 Sampling . 8
7 Test specimens . 8
7.1 Species of wood . 8
7.2 Wood quality . 8
7.3 Provision of test specimens . 8
7.4 Dimensions of test specimens . 8
7.5 Number of test specimens . 9
8 Procedure. 9
8.1 Preparation of the test specimens . 9
8.1.1 Conditioning of test specimens before treatment . 9
8.1.2 Treatment of the test specimens . 9
8.1.3 Drying and conditioning of the test specimens after treatment . 10
8.2 Exposure of the test specimens to the insects . 11
8.3 Conditions and duration of the test . 11
8.4 Examination of the test specimens . 11
9 Validity of test . 12
10 Expression of results . 12
10.1 Assessment of the protective effectiveness . 12
10.2 Toxic values . 12
11 Test report . 12
Annex A (informative) Example of a test report . 14
Annex B (informative) Identification of sex of test insects (Anobium punctatum). 16
Annex C (informative) Culturing technique for Anobium punctatum. 17
Annex D (informative) Environmental, health and safety precautions within
chemical/biological laboratory . 20
Bibliography . 21

European foreword
This document (prEN 49-2:2025) has been prepared by Technical Committee CEN/TC 38 “Biological
durability of wood and wood-based products”, the secretariat of which is held by SIS.
This document is currently submitted to the CEN Enquiry.
This document will supersede EN 49-2:2015.
Significant technical differences between this document and EN 49-2:2015 are as follows:
a) requirements for temperature and humidity were aligned with other insect test standards (5.3.1;
5.3.5);
b) imaging facilities for non-destructive recordings of insect tunnels inside test specimens were
generalized (5.3.13);
c) storage limitations for wood from which test specimens are to be made from have been cancelled
(7.2).
NOTE Test results obtained according to earlier versions of this document or when the tests had started
before this version of EN 49-1 was published are considered as valid.
EN 49, Wood preservatives ― Determination of the protective effectiveness against Anobium punctatum
(De Geer) by egg-laying and larval survival, consists of two parts:
— Part 1: Application by surface treatment (Laboratory method);
— Part 2: Application by impregnation (Laboratory method).
EN 49-1 is required to enable effectiveness assessments of wood preservatives which are intended to
be applied by surface treatment and EN 49-2 those which are intended to be applied by impregnation.

Introduction
This document describes a laboratory method of testing which gives a basis for assessment of the
effectiveness of a wood preservative, against Anobium punctatum. It allows the determination of the
concentration at which the product prevents the development of infestation from egg laying.
The method simulates conditions which can occur in practice on timber which has been treated some
time previously with a deeply penetrating wood preservative and on which eggs of Anobium punctatum
are laid.
This laboratory method provides one criterion by which the value of a product can be assessed. In
making this assessment the methods by which the preservative may be applied should be taken into
account. It is further recommended that results from this test should be supplemented by those from
other appropriate tests, and above all by comparison with practical experience.
When products which are very active at low concentrations are used it is very important to take
suitable precautions to isolate and separate, as far as possible, operations involving chemical products,
other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include
the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special
training for personnel (see also Annex D for environmental, health and safety precautions).

1 Scope
This document specifies a method for the determination of the protective effectiveness or the toxic
values of a wood preservative against Anobium punctatum (De Geer) by egg-laying and larval survival in
wood which has been treated previously by full impregnation. This method is applicable to:
— water-insoluble chemicals which are being studied as active insecticides;
— organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates;
— organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of
concentrates;
— water-soluble materials, for example salts.
The method is applicable whether or not the test specimens have been subjected to appropriate ageing
procedures.
2 Normative reference
The following documents, in whole or in part, are normatively referenced in this document and are
indispensable for its application. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any amendments) applies.
EN 73, Durability of wood and wood-based products — Accelerated ageing of treated wood prior to
biological testing — Evaporative ageing procedure
EN 84, Durability of wood and wood-based products — Accelerated ageing of treated wood prior to
biological testing — Leaching procedure
EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
representative sample
sample having its physical or chemical characteristics identical to the volumetric average
characteristics of the total volume being sampled
3.2
supplier
sponsor of the test (person or company providing the sample of wood preservative to be tested)
4 Principle
The treated test specimens are exposed to gravid females of Anobium punctatum. The numbers of eggs
laid, the numbers of eggs hatched, and the numbers of the surviving larvae are compared with those in
untreated control test specimens. If the preservative has been prepared in
...