ISO 21702:2019

INTERNATIONAL ISO
STANDARD 21702
First edition
2019-05
Measurement of antiviral activity
on plastics and other non-porous
surfaces
Mesure de l'activité antivirale sur les matières plastiques et autres
surfaces non poreuses
Reference number
©
ISO 2019
© ISO 2019
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ii © ISO 2019 – All rights reserved

Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Materials . 2
4.1 Virus and host cell to be used for the tests . 2
4.2 Reagents. 2
4.3 Culture medium and solutions . 2
4.3.1 General. 2
4.3.2 Eagle's minimum essential medium (EMEM) . 2
4.3.3 RPMI 1640 medium . 2
4.3.4 7,5 % sodium bicarbonate solution . 3
4.3.5 Formaldehyde solution. 3
4.3.6 Methylene blue solution . 3
4.3.7 Inactivated fetal bovine serum (FBS) . 3
4.3.8 Growth medium . 3
4.3.9 Maintenance medium . 3
4.3.10 Double concentration of the maintenance medium . 4
4.3.11 Phosphate buffered saline [PBS (-)] . 4
4.3.12 Trypsin derived from beef pancreas and PBS (-) solution . 4
4.3.13 Trypsin EDTA solution . 4
4.3.14 DEAE-dextran solution . 4
4.3.15 Agar medium for plaque assay . 5
4.3.16 Soybean casein digest broth with lecithin and polyoxyethylene sorbitan
monooleate (SCDLP broth) . 5
5 Apparatus . 5
6 Preparation . 6
6.1 Restoration of host cell from cryopreservation . 6
6.2 Subculture of host cell . 7
6.3 Cell culture for measuring virus infectivity titer . 7
6.4 Preparation of test inoculums. 7
6.4.1 Influenza virus . 7
6.4.2 Feline calicivirus. 8
6.5 Preparation of test specimens . 8
6.6 Control test . 9
6.6.1 General. 9
6.6.2 Verification of cytotoxic effect on host cell . 9
6.6.3 Verification of cell sensitivity to virus and the inactivation of antiviral activity . 9
7 Test procedure .11
7.1 Preparation of test specimen .11
7.2 Inoculation of test specimens .11
7.3 Contact of the inoculated test specimens.12
7.4 Recovery of virus from test specimens .12
7.4.1 Test specimens immediately after inoculation .12
7.4.2 Test specimens after contact .12
7.5 Determining the infectivity titer of virus by plaque assay .12
8 Expression of results .13
8.1 Determination of the infectivity titer of virus .13
8.2 Conditions for a valid test .13
8.3 Calculation of the antiviral activity .14
8.4 Effectiveness of the antiviral agent .14
9 Repeatability and reproducibility .14
10 Test report .14
Annex A (informative) Composition of media .16
Annex B (informative) Repeatability and reproducibility .18
Bibliography .20
iv © ISO 2019 – All rights reserved

Foreword
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This document was prepared by Technical Committee ISO/TC 61, Plastics, Subcommittee SC 6, Ageing,
chemical and environmental resistance.
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Introduction
Antibacterial-treated porous and non-porous products have been widely accepted and used among
general consumers as their new choices to purchase for the additional function, which are different
from what traditional materials had in terms of material protection.
Recently, antiviral-treated porous and non-porous products have been also in the market.
The measuring test method of antibacterial activity on non-porous products is described in ISO 22196.
The measuring test method of antibacterial activity on porous products (textiles) is described in
ISO 20743.
The measuring test method of antiviral activity on porous products (textiles) is described in ISO 18184.
This document is the test method of antiviral activity on non-porous products. It is written based on
ISO 22196 and ISO 18184.
In ISO 22196, the scope has been expanded to include surfaces made of plastics and other non-porous
materials, thus this document is intended to be applicable to products such as plastics, coating mater
...