ISO 27447:2019

INTERNATIONAL ISO
STANDARD 27447
Second edition
2019-07
Fine ceramics (advanced ceramics,
advanced technical ceramics) — Test
method for antibacterial activity
of semiconducting photocatalytic
materials
Céramiques techniques — Méthode d'essai de l'activité
antibactérienne des matériaux photocatalytiques semiconducteurs
Reference number
©
ISO 2019
© ISO 2019
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ii © ISO 2019 – All rights reserved

Contents Page
Foreword .v
Introduction .vii
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols . 2
5 Principle . 3
6 Materials . 4
6.1 Bacteria used and preparation for tests . 4
6.1.1 Film cover method. 4
6.1.2 Glass cover method . 4
6.1.3 Bacteria preparation. 4
6.2 Chemicals and implements . 5
6.2.1 General. 5
6.2.2 1/500 nutrient broth . 5
6.2.3 Nutrient broth . 5
6.2.4 Nutrient agar . 5
6.2.5 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP). 5
6.2.6 Physiological saline solution . 5
6.2.7 Physiological saline solution for washout . 5
6.2.8 Non-ionic surfactant . 6
7 Apparatus . 6
7.1 Test equipment . 6
7.2 Cover film . 6
7.3 Cover glass . 6
7.4 Moisture preservation glass . 7
7.5 Glass tube or glass rod . 7
7.6 Paper filter . 7
7.7 Light source . 7
7.8 UV radiometer . 7
7.9 Punched metal sheet . 7
8 Test piece . 9
8.1 Film cover method . 9
8.2 Glass cover method . 9
9 Procedure. 9
9.1 General . 9
9.2 Film cover method .11
9.3 Glass cover method .11
9.4 UV irradiation condition .12
9.5 Measurement of the number of living bacteria .13
10 Calculation .14
10.1 General .14
10.2 Film cover method .14
10.3 Glass cover method .15
11 Test report .16
Annex A (informative) Examples of test results .18
Annex B (informative) Reference data of cover films and cover glasses .20
Annex C (informative) Reference data of damage caused by ultraviolet to bacteria .22
Bibliography .24
iv © ISO 2019 – All rights reserved

Foreword
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This document was prepared by Technical Committee ISO/TC 206, Fine ceramics. This second edition
cancels and replaces the first edition (ISO 27447:2009), which has been technically revised. The main
changes to the previous edition are as follows:
— updating of reference document and cross-references;
— replacement of “adhesive” with “cover” throughout;
— clarification of definition of “photocatalyst antibacterial activity value” (3.4, 3.5, 3.6, 3.7) in Clause 3;
— deletion of a definition of “fluorescent UV lamp” in Clause 3 due to updating of the reference document
ISO 10677;
— inclusion of a statement in Clause 5 regarding treatment of results measured by the viable bacterial
count method;
— NOTE 1 changed to body text in 6.1.3;
— revision of “storage period of 1/500 nutrient broth” from 1 month ago to 1 week ago in 6.2.2
(formerly 6.2.1);
— addition of a new subclause, 6.2.1, renumbering of subsequent subclauses and updating of cross-
references in Clause 6;
— addition of a new subclause, 7.1, and renumbering of subsequent subclauses in Clause 7;
— revision of Figure 1, Figure 4 and Figure 5;
— addition of “paper filter” apparatus in 7.6;
— replacement of “black light fluorescent lamp” with “light source” in 7.7 (formerly 7.5) and revision of
a statement in 7.7 that the light source shall be 351BLB or 351BL as specified in ISO 10677;
— replacement of “ultraviolet light radiation mater” with “UV radiometer” in 7.8 (formerly 7.6) and
inclusion of a statement in 7.8 that the UV radiometer shall be used as specified in ISO 10677;
— NOTE changed to body text in 8.1;
— addition of a new subclause, 9.1, and renumbering of subsequent subclauses in Clause 9;
— revision of storage time of “the bacteria suspension in case of not using immediately” from 4 h to 2 h
in 9.2.1(formerly 9.1.1);
...