Plastics - Assessment of the effectiveness of fungistatic compounds in plastics formulations

ISO 16869:2008 specifies a method for determining the effectiveness of fungistatic compounds in protecting susceptible ingredients like plasticizers, stabilizers, etc., in plastics formulations. The method demonstrates whether or not a plastic product is actively protected against fungal attack. The evaluation is by visual examination. The test is applicable to any articles made of plastic that are in the form of films or plates no thicker than 10 mm. In addition, porous materials such as plastic foams may be tested provided that they are in the above-mentioned form. A minimum diffusion of the fungicides that migrate out of the matrix is necessary with this procedure. In contrast to ISO 846, the test films are not sprayed with a fungal spore suspension but covered with a layer of test agar containing spores. It has been found that this leads to a better distribution of the spores as well as providing a good supply of water necessary for spore germination on plastic surfaces that are normally hydrophobic.

Plastiques β€” Γ‰valuation de l'efficacitΓ© des composΓ©s fongistatiques dans les formulations de plastiques

General Information

Status
Published
Publication Date
13-May-2008
Current Stage
9093 - International Standard confirmed
Start Date
14-Jun-2021
Completion Date
13-Dec-2025

Relations

Effective Date
15-Apr-2008

Overview

ISO 16869:2008 - Plastics: Assessment of the effectiveness of fungistatic compounds in plastics formulations specifies a laboratory method to determine whether fungistatic additives incorporated into plastics protect susceptible ingredients (plasticizers, stabilizers, etc.) against fungal attack. The test applies to plastic articles in the form of films or plates (thickness ≀ 10 mm) and certain porous forms (e.g., foams) when cut to the required shape. Evaluation is by visual examination after exposure to fungal spores applied in an agar overlay, which promotes uniform spore distribution and water availability on hydrophobic plastic surfaces.

Key Topics and Requirements

  • Test principle: Specimens are overlaid with a thin layer of nutrient‑salt agar containing a mixed fungal spore suspension. Growth (or inhibition) on and around the specimen indicates absence (or presence) of effective fungistatic action; migration of fungicides into agar can produce inhibition zones.
  • Specimen dimensions: Round discs punched to 1–4 cm diameter, thickness not exceeding 10 mm; at least three replicates per material.
  • Organisms: Standard test fungi include Aspergillus niger, Chaetomium globosum, Paecilomyces variotii, Penicillium funiculosum and Trichoderma longibrachiatum (reference strains from national culture collections).
  • Culture media & conditions: Nutrient‑salt agar (pH ~5.5) is used for overlay; malt‑extract and Chaetomium agars are specified for cultivation. Plates are incubated at 24 Β°C Β± 1 Β°C. Example procedural details (sterilization methods, media recipes, centrifugation and spore preparation) are included in the standard.
  • Assessment: Visual scoring of fungal growth and any zone of inhibition; the method requires sufficient diffusion/migration of fungistat to be detected.
  • Safety & competence: Handling potentially hazardous microorganisms requires trained personnel and appropriate biosafety practices.

Applications and Who Uses It

  • Plastics manufacturers evaluating additive performance during formulation development.
  • Additive (fungistat) suppliers verifying efficacy and supporting product claims.
  • Quality control and testing laboratories performing routine batch verification or comparative testing.
  • R&D teams optimizing plasticizer/stabilizer packages for resistance to biodeterioration.
  • Regulatory and procurement specialists assessing material fitness for use in humidity-prone or biological-exposure environments.

Practical benefits include comparative efficacy data, detection of inadequate protection in finished articles, and insight into fungistat migration behavior (via inhibition zones).

Related Standards

  • ISO 846 (assessment of resistance to mould growth - different inoculation approach)
  • ISO 291:2008 (conditioning atmospheres)
  • IEC 60068‑2‑10 and ISO 7218 (guidance on personnel safety and microbiological practice)

Keywords: ISO 16869:2008, fungistatic compounds, plastics testing, fungal resistance, nutrient-salt agar, agar overlay, plastic films, inhibition zone, visual assessment.

Standard

ISO 16869:2008 - Plastics -- Assessment of the effectiveness of fungistatic compounds in plastics formulations

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Frequently Asked Questions

ISO 16869:2008 is a standard published by the International Organization for Standardization (ISO). Its full title is "Plastics - Assessment of the effectiveness of fungistatic compounds in plastics formulations". This standard covers: ISO 16869:2008 specifies a method for determining the effectiveness of fungistatic compounds in protecting susceptible ingredients like plasticizers, stabilizers, etc., in plastics formulations. The method demonstrates whether or not a plastic product is actively protected against fungal attack. The evaluation is by visual examination. The test is applicable to any articles made of plastic that are in the form of films or plates no thicker than 10 mm. In addition, porous materials such as plastic foams may be tested provided that they are in the above-mentioned form. A minimum diffusion of the fungicides that migrate out of the matrix is necessary with this procedure. In contrast to ISO 846, the test films are not sprayed with a fungal spore suspension but covered with a layer of test agar containing spores. It has been found that this leads to a better distribution of the spores as well as providing a good supply of water necessary for spore germination on plastic surfaces that are normally hydrophobic.

ISO 16869:2008 specifies a method for determining the effectiveness of fungistatic compounds in protecting susceptible ingredients like plasticizers, stabilizers, etc., in plastics formulations. The method demonstrates whether or not a plastic product is actively protected against fungal attack. The evaluation is by visual examination. The test is applicable to any articles made of plastic that are in the form of films or plates no thicker than 10 mm. In addition, porous materials such as plastic foams may be tested provided that they are in the above-mentioned form. A minimum diffusion of the fungicides that migrate out of the matrix is necessary with this procedure. In contrast to ISO 846, the test films are not sprayed with a fungal spore suspension but covered with a layer of test agar containing spores. It has been found that this leads to a better distribution of the spores as well as providing a good supply of water necessary for spore germination on plastic surfaces that are normally hydrophobic.

ISO 16869:2008 is classified under the following ICS (International Classification for Standards) categories: 83.080.01 - Plastics in general. The ICS classification helps identify the subject area and facilitates finding related standards.

ISO 16869:2008 has the following relationships with other standards: It is inter standard links to ISO 16869:2001. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.

ISO 16869:2008 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.

Standards Content (Sample)


INTERNATIONAL ISO
STANDARD 16869
Second edition
2008-06-01
Plastics β€” Assessment of the
effectiveness of fungistatic compounds
in plastics formulations
Plastiques β€” Γ‰valuation de l'efficacitΓ© des composΓ©s fongistatiques
dans les formulations de plastiques

Reference number
Β©
ISO 2008
PDF disclaimer
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Β©  ISO 2008
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
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Published in Switzerland
ii Β© ISO 2008 – All rights reserved

Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 2
4 Principle. 2
5 Apparatus and materials. 2
5.1 Apparatus . 2
5.2 Culture media and reagents . 3
5.3 Organisms and cultivation. 5
6 Test specimens . 5
6.1 Shape and dimensions. 5
6.2 Number of specimens . 5
7 Preparation of specimens. 5
7.1 Cleaning. 5
7.2 Labelling and storage. 5
8 Procedure . 6
8.1 Test temperature. 6
8.2 Filling the Petri dishes. 6
8.3 Arrangement of test specimens . 6
8.4 Inoculation of the test specimens. 6
8.4.1 Preparation of the spore suspension . 6
8.4.2 Inoculation of the nutrient-salt overlay agar. 6
8.4.3 Overlay of specimen. 6
8.4.4 Incubation. 7
8.4.5 Viability control . 7
9 Assessment of fungal growth . 7
10 Expression of results . 7
11 Precision and bias . 8
12 Test report . 8
Bibliography . 9

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 16869 was prepared by Technical Committee ISO/TC 61, Plastics, Subcommittee SC 6, Ageing, chemical
and environmental resistance.
This second edition cancels and replaces the first edition (ISO 16869:2001), of which it constitutes a minor
technical revision. The main changes are an increase in the maximum diameter of the test specimen to 4 cm
(see 6.1) and the introduction of centrifuging operations in the preparation of the spore suspension in 8.4.1.
iv Β© ISO 2008 – All rights reserved

Introduction
It is a well known phenomenon that plasticizers as well as other ingredients in plastics formulations can be
attacked by bacteria, yeasts and fungi, the latter being the most important deteriogens. Microbial attack results
in a reduction of the quality of the plastic, causing embrittlement as well as discoloration. This deterioration is
of economic importance.
The prevention of fungal attack can be achieved by the incorporation of a fungistatic compound into the
formulation. The function of this fungistat is to inhibit the growth of any fungi present on the surface of the
plastic product.
The method described in this International Standard determines the effectiveness of fungistatic compounds
incorporated into the plastic against the fungi used in the test.

INTERNATIONAL STANDARD ISO 16869:2008(E)

Plastics β€” Assessment of the effectiveness of fungistatic
compounds in plastics formulations
WARNING β€” Handling and manipulation of microorganisms that are potentially hazardous requires a
high degree of technical competence and may be subject to current national legislation and
regulations. Only personnel trained in microbiological techniques should carry out such tests. Codes
of practice for disinfection, sterilization and personal hygiene must be strictly observed.
It is recommended that workers consult IEC 60068-2-10:2005, Annex A β€œDanger to personnel”, and
ISO 7218, Microbiology of food and animal feeding stuffs β€” General requirements and guidance for
microbiological examinations.
1 Scope
This International Standard specifies a method for determining the effectiveness of fungistatic compounds in
protecting susceptible ingredients like plasticizers, stabilizers, etc., in plastics formulations. The method
demonstrates whether or not a plastic product is actively protected against fungal attack.
The evaluation is by visual examination.
The test is applicable to any articles made of plastic that are in the form of films or plates no thicker than
10 mm. In addition, porous materials such as plastic foams may be tested provided that they are in the above-
mentioned form.
A minimum diffusion of the fungicides that migrate out of the matrix is necessary with this procedure.
In contrast to ISO 846, the test films are not sprayed with a fungal spore suspension but covered with a layer
of test agar containing spores. It has been found that this leads to a better distribution of the spores as well as
providing a good supply of water necessary for spore germination on plastic surfaces that are normally
hydrophobic.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 291:2008, Plastics β€” Standard atmospheres for conditioning and testing
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
plastic susceptible to fungal attack
plastic material that contains in its formulation one or more nutrients that support fungal growth
3.2
fungistat
compound that prevents fungal growth on a material that is normally susceptible to fungal attack
4 Principle
Test specimens are exposed to a suspension of mixed fungal spores. The spores are applied to the surface of
the test specimen in a thin layer of an agar medium without an added carbon source. In this way, uniform
distribution of the spores is achieved as well as an optimum supply of water.
The absence of fungistatic agents in the plastic material will lead to germination of the fungal spores and initial
growth. When the ingredients in the material are susceptible to fungal attack and no active fungistat is
included in the formulation, further growth and sporulation will occur over and around the test specimen.
The presence of an active fungistat in the material will lead to suppression of spore germination and initial
growth in the area over and around the test specimen. Fungistatic agents can migrate into the agar around the
test specimen, thereby suppressing germination and appearing to give an increased zone of inhibition.
Although not relevant to the interpretation of the test results, the inhibition zone can be an indication of the
behaviour of the fungistat under test.
5 Apparatus and materials
5.1 Apparatus
Sterilize all glassware and all parts of the rest of the apparatus that will come into contact with the culture
media and/or reagents (except those which are supplied sterile) by one of the following methods:
method A: autoclave (see 5.1.2) at 121 Β°C for a minimum of 15 min;
method B: use a dry-heat sterilizer (see 5.1.2) at 180 Β°C for at least 30 min, at 170 Β°C for at
...


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БВАНДАРВ 16869
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Plastics – Assessment of the effectiveness of fungistatic compounds in
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Π‘ΠΎΠ΄Π΅Ρ€ΠΆΠ°Π½ΠΈΠ΅ Π‘Ρ‚Ρ€Π°Π½ΠΈΡ†Π°
ΠŸΡ€Π΅Π΄ΠΈΡΠ»ΠΎΠ²ΠΈΠ΅ .iv
Π’Π²Π΅Π΄Π΅Π½ΠΈΠ΅ .v
1  ΠžΠ±Π»Π°ΡΡ‚ΡŒ примСнСния .1
2  НормативныС ссылки .1
3  Π’Π΅Ρ€ΠΌΠΈΠ½Ρ‹ ΠΈ опрСдСлСния .1
4  Π‘ΡƒΡ‰Π½ΠΎΡΡ‚ΡŒ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°.2
5  Аппаратура ΠΈ ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹.2
5.1  Аппаратура.2
5.2  ΠŸΠΈΡ‚Π°Ρ‚Π΅Π»ΡŒΠ½Π°Ρ срСда ΠΈ Ρ€Π΅Π°ΠΊΡ‚ΠΈΠ²Ρ‹ .3
5.3  ΠžΡ€Π³Π°Π½ΠΈΠ·ΠΌΡ‹ ΠΈ Π²Ρ‹Ρ€Π°Ρ‰ΠΈΠ²Π°Π½ΠΈΠ΅ .5
6  испытуСмыС ΠΎΠ±Ρ€Π°Π·Ρ†Ρ‹.5
6.1  Π€ΠΎΡ€ΠΌΠ° ΠΈ Ρ€Π°Π·ΠΌΠ΅Ρ€Ρ‹ .5
6.2  Число ΠΎΠ±Ρ€Π°Π·Ρ†ΠΎΠ² для испытания.5
7  ΠŸΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠ° ΠΎΠ±Ρ€Π°Π·Ρ†ΠΎΠ² для испытания .6
7.1  ΠžΡ‡ΠΈΡΡ‚ΠΊΠ° .6
7.2  Π­Ρ‚ΠΈΠΊΠ΅Ρ‚ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΈ Ρ…Ρ€Π°Π½Π΅Π½ΠΈΠ΅ .6
8  ΠŸΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠ΅ испытания.6
8.1  Π’Π΅ΠΌΠΏΠ΅Ρ€Π°Ρ‚ΡƒΡ€Π° испытания.6
8.2  НаполнСниС Ρ‡Π°ΡˆΠ΅ΠΊ ΠŸΠ΅Ρ‚Ρ€ΠΈ .6
8.3  РасполоТСниС испытуСмых ΠΎΠ±Ρ€Π°Π·Ρ†ΠΎΠ² .6
8.4  ПосСв Π½Π° испытуСмыС ΠΎΠ±Ρ€Π°Π·Ρ†Ρ‹.6
8.4.1  ΠŸΡ€ΠΈΠ³ΠΎΡ‚ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ суспСнзии спор.6
8.4.2  ПосСв слоя ΠΏΠΈΡ‚Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎ-солСвого Π°Π³Π°Ρ€Π° .7
8.4.3  НанСсСниС Π½Π° ΠΎΠ±Ρ€Π°Π·Π΅Ρ†.7
8.4.4  Π˜Π½ΠΊΡƒΠ±Π°Ρ†ΠΈΡ .7
8.4.5  ΠšΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒ ТизнСспособности .7
9  ΠžΡ†Π΅Π½ΠΊΠ° роста Π³Ρ€ΠΈΠ±ΠΊΠΎΠ² .7
10  ΠžΠ±Ρ€Π°Π±ΠΎΡ‚ΠΊΠ° Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚ΠΎΠ².8
11  ΠŸΡ€Π΅Ρ†ΠΈΠ·ΠΈΠΎΠ½Π½ΠΎΡΡ‚ΡŒ ΠΈ систСматичСская ΠΏΠΎΠ³Ρ€Π΅ΡˆΠ½ΠΎΡΡ‚ΡŒ .8
12  ΠŸΡ€ΠΎΡ‚ΠΎΠΊΠΎΠ» испытания.8
Библиография.9

ΠŸΡ€Π΅Π΄ΠΈΡΠ»ΠΎΠ²ΠΈΠ΅
ΠœΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Π°Ρ организация ΠΏΠΎ стандартизации (ISO) являСтся всСмирной Ρ„Π΅Π΄Π΅Ρ€Π°Ρ†ΠΈΠ΅ΠΉ Π½Π°Ρ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½Ρ‹Ρ…
ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΉ ΠΏΠΎ стандартизации (ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚ΠΎΠ²-Ρ‡Π»Π΅Π½ΠΎΠ² ISO). Π Π°Π·Ρ€Π°Π±ΠΎΡ‚ΠΊΠ° ΠΌΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹Ρ… стандартов
ΠΎΠ±Ρ‹Ρ‡Π½ΠΎ осущСствляСтся тСхничСскими ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚Π°ΠΌΠΈ ISO. ΠšΠ°ΠΆΠ΄Ρ‹ΠΉ ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚-Ρ‡Π»Π΅Π½, заинтСрСсованный Π²
Π΄Π΅ΡΡ‚Π΅Π»ΡŒΠ½ΠΎΡΡ‚ΠΈ, для ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠΉ Π±Ρ‹Π» создан тСхничСский ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚, ΠΈΠΌΠ΅Π΅Ρ‚ ΠΏΡ€Π°Π²ΠΎ Π±Ρ‹Ρ‚ΡŒ прСдставлСнным Π² этом
ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚Π΅. ΠœΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹Π΅ ΠΏΡ€Π°Π²ΠΈΡ‚Π΅Π»ΡŒΡΡ‚Π²Π΅Π½Π½Ρ‹Π΅ ΠΈ Π½Π΅ΠΏΡ€Π°Π²ΠΈΡ‚Π΅Π»ΡŒΡΡ‚Π²Π΅Π½Π½Ρ‹Π΅ ΠΎΡ€Π³Π°Π½ΠΈΠ·Π°Ρ†ΠΈΠΈ, ΠΈΠΌΠ΅ΡŽΡ‰ΠΈΠ΅ связи с
ISO, Ρ‚Π°ΠΊΠΆΠ΅ ΠΏΡ€ΠΈΠ½ΠΈΠΌΠ°ΡŽΡ‚ участиС Π² Ρ€Π°Π±ΠΎΡ‚Π°Ρ…. ISO Ρ€Π°Π±ΠΎΡ‚Π°Π΅Ρ‚ Π² тСсном сотрудничСствС с ΠœΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½ΠΎΠΉ
элСктротСхничСской комиссиСй (IEC) ΠΏΠΎ всСм вопросам стандартизации Π² области элСктротСхники.
ΠœΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹Π΅ стандарты Ρ€Π°Π·Ρ€Π°Π±Π°Ρ‚Ρ‹Π²Π°ΡŽΡ‚ΡΡ Π² соотвСтствии с ΠΏΡ€Π°Π²ΠΈΠ»Π°ΠΌΠΈ, установлСнными Π²
Π”ΠΈΡ€Π΅ΠΊΡ‚ΠΈΠ²Π°Ρ… ISO/IEC, Π§Π°ΡΡ‚ΡŒ 2.
Основная Π·Π°Π΄Π°Ρ‡Π° тСхничСских ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚ΠΎΠ² состоит Π² ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²ΠΊΠ΅ ΠΌΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹Ρ… стандартов. ΠŸΡ€ΠΎΠ΅ΠΊΡ‚Ρ‹
ΠΌΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹Ρ… стандартов, ΠΎΠ΄ΠΎΠ±Ρ€Π΅Π½Π½Ρ‹Π΅ тСхничСскими ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚Π°ΠΌΠΈ, Ρ€Π°ΡΡΡ‹Π»Π°ΡŽΡ‚ΡΡ ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚Π°ΠΌ-Ρ‡Π»Π΅Π½Π°ΠΌ
Π½Π° голосованиС. Π˜Ρ… ΠΎΠΏΡƒΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½ΠΈΠ΅ Π² качСствС ΠΌΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹Ρ… стандартов Ρ‚Ρ€Π΅Π±ΡƒΠ΅Ρ‚ одобрСния, ΠΏΠΎ
мСньшСй ΠΌΠ΅Ρ€Π΅, 75 % ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚ΠΎΠ²-Ρ‡Π»Π΅Π½ΠΎΠ², ΠΏΡ€ΠΈΠ½ΠΈΠΌΠ°ΡŽΡ‰ΠΈΡ… участиС Π² голосовании.
Π‘Π»Π΅Π΄ΡƒΠ΅Ρ‚ ΠΈΠΌΠ΅Ρ‚ΡŒ Π² Π²ΠΈΠ΄Ρƒ, Ρ‡Ρ‚ΠΎ Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ элСмСнты этого Π΄ΠΎΠΊΡƒΠΌΠ΅Π½Ρ‚Π° ΠΌΠΎΠ³ΡƒΡ‚ Π±Ρ‹Ρ‚ΡŒ ΠΎΠ±ΡŠΠ΅ΠΊΡ‚ΠΎΠΌ ΠΏΠ°Ρ‚Π΅Π½Ρ‚Π½Ρ‹Ρ… ΠΏΡ€Π°Π².
ISO Π½Π΅ Π΄ΠΎΠ»ΠΆΠ΅Π½ нСсти ΠΎΡ‚Π²Π΅Ρ‚ΡΡ‚Π²Π΅Π½Π½ΠΎΡΡ‚ΡŒ Π·Π° ΠΈΠ΄Π΅Π½Ρ‚ΠΈΡ„ΠΈΠΊΠ°Ρ†ΠΈΡŽ ΠΊΠ°ΠΊΠΎΠ³ΠΎ-Π»ΠΈΠ±ΠΎ ΠΎΠ΄Π½ΠΎΠ³ΠΎ ΠΈΠ»ΠΈ всСх ΠΏΠ°Ρ‚Π΅Π½Ρ‚Π½Ρ‹Ρ… ΠΏΡ€Π°Π².
ISO 16869 Π±Ρ‹Π» ΠΏΠΎΠ΄Π³ΠΎΡ‚ΠΎΠ²Π»Π΅Π½ ВСхничСским ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚ΠΎΠΌ ISO/TC 61, ΠŸΠ»Π°ΡΡ‚ΠΌΠ°ΡΡΡ‹, ΠŸΠΎΠ΄ΠΊΠΎΠΌΠΈΡ‚Π΅Ρ‚ΠΎΠΌ SC 6,
Π‘Ρ‚Π°Ρ€Π΅Π½ΠΈΠ΅, ΡΡ‚ΠΎΠΉΠΊΠΎΡΡ‚ΡŒ ΠΊ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ химичСских вСщСств ΠΈ ΠΎΠΊΡ€ΡƒΠΆΠ°ΡŽΡ‰ΠΈΡ… условий.
НастоящСС Π²Ρ‚ΠΎΡ€ΠΎΠ΅ ΠΈΠ·Π΄Π°Π½ΠΈΠ΅ отмСняСт ΠΈ замСняСт ΠΏΠ΅Ρ€Π²ΠΎΠ΅ ΠΈΠ·Π΄Π°Π½ΠΈΠ΅ (ISO 16869:2001), ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠ΅ ΠΏΡ€ΠΎΡˆΠ»ΠΎ
тСхничСский пСрСсмотр. Π’ число Π³Π»Π°Π²Π½Ρ‹Ρ… ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π²Ρ…ΠΎΠ΄ΠΈΡ‚ ΡƒΠ²Π΅Π»ΠΈΡ‡Π΅Π½ΠΈΠ΅ максимального Π΄ΠΈΠ°ΠΌΠ΅Ρ‚Ρ€Π°
испытуСмого ΠΎΠ±Ρ€Π°Π·Ρ†Π° Π΄ΠΎ 4 см (см. 6.1) ΠΈ Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ ΠΎΠΏΠ΅Ρ€Π°Ρ†ΠΈΠΉ цСнтрифугирования ΠΏΡ€ΠΈ ΠΏΡ€ΠΈΠ³ΠΎΡ‚ΠΎΠ²Π»Π΅Π½ΠΈΠΈ
суспСнзии спор Π² 8.4.1.
iv Β© ISO 2008 – ВсС ΠΏΡ€Π°Π²Π° ΡΠΎΡ…Ρ€Π°Π½ΡΡŽΡ‚ΡΡ

Π’Π²Π΅Π΄Π΅Π½ΠΈΠ΅
Π₯ΠΎΡ€ΠΎΡˆΠΎ извСстно явлСниС воздСйствия Π½Π° пластификаторы, Π° Ρ‚Π°ΠΊΠΆΠ΅ Π΄Ρ€ΡƒΠ³ΠΈΠ΅ ΠΈΠ½Π³Ρ€Π΅Π΄ΠΈΠ΅Π½Ρ‚Ρ‹, входящиС Π²
состав пластмасс Π±Π°ΠΊΡ‚Π΅Ρ€ΠΈΠΉ, Π΄Ρ€ΠΎΠΆΠΆΠ΅ΠΉ ΠΈ плСсСнСй, послСдниС ΠΈΠ· ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… ΡΠ²Π»ΡΡŽΡ‚ΡΡ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅
Ρ€Π°Π·Ρ€ΡƒΡˆΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ΠΌΠΈ. ВоздСйствиС ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠΎΠ² ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ ΡƒΡ…ΡƒΠ΄ΡˆΠ΅Π½ΠΈΡŽ качСства пластмассы, Π΅Π΅
ΠΎΡ…Ρ€ΡƒΠΏΡ‡ΠΈΠ²Π°Π½ΠΈΡŽ, Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΎΠ±Π΅ΡΡ†Π²Π΅Ρ‡ΠΈΠ²Π°Π½ΠΈΡŽ. Π­Ρ‚ΠΎ ΠΈΠΌΠ΅Π΅Ρ‚ экономичСскоС Π·Π½Π°Ρ‡Π΅Π½ΠΈΠ΅.
ΠŸΡ€Π΅Π΄ΠΎΡ‚Π²Ρ€Π°Ρ‰Π΅Π½ΠΈΠ΅ воздСйствия плСсСни ΠΌΠΎΠΆΠ½ΠΎ ΠΎΡΡƒΡ‰Π΅ΡΡ‚Π²ΠΈΡ‚ΡŒ ΠΏΡƒΡ‚Π΅ΠΌ Π²ΠΊΠ»ΡŽΡ‡Π΅Π½ΠΈΡ Π² состав пластмассы
фунгистатичСского вСщСства. Ѐункция Ρ‚Π°ΠΊΠΎΠ³ΠΎ Ρ„ΡƒΠ½Π³ΠΈΡ†ΠΈΠ΄Π° Π·Π°ΠΊΠ»ΡŽΡ‡Π°Π΅Ρ‚ΡΡ Π² ΠΈΠ½Π³ΠΈΠ±ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ роста плСсСни ,
ΠΏΡ€ΠΈΡΡƒΡ‚ΡΡ‚Π²ΡƒΡŽΡ‰Π΅ΠΉ Π½Π° повСрхности пластмассового ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Π°.
ΠœΠ΅Ρ‚ΠΎΠ΄, описанный Π² Π΄Π°Π½Π½ΠΎΠΌ ΠΌΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½ΠΎΠΌ стандартС, опрСдСляСт ΡΡ„Ρ„Π΅ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ фунгистатичСских
соСдинСний, Π²ΠΊΠ»ΡŽΡ‡Π΅Π½Π½Ρ‹Ρ… Π² состав пластмассы, ΠΏΡ€ΠΎΡ‚ΠΈΠ² плСсСни, ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΠ΅ΠΌΠΎΠΉ Π² испытании.

ΠœΠ•Π–Π”Π£ΠΠΠ ΠžΠ”ΠΠ«Π™ БВАНДАРВ ISO 16869:2008(R)

ΠŸΠ»Π°ΡΡ‚ΠΌΠ°ΡΡΡ‹. ΠžΡ†Π΅Π½ΠΊΠ° эффСктивности фунгистатичСских
соСдинСний Π² составС пластмасс
ΠŸΠ Π•Π”Π£ΠŸΠ Π•Π–Π”Π•ΠΠ˜Π• β€” Π Π°Π±ΠΎΡ‚Π° с ΠΏΠΎΡ‚Π΅Π½Ρ†ΠΈΠ°Π»ΡŒΠ½ΠΎ опасными ΠΌΠΈΠΊΡ€ΠΎΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠ°ΠΌΠΈ Ρ‚Ρ€Π΅Π±ΡƒΠ΅Ρ‚ высокой
стСпСни ΠΊΠΎΠΌΠΏΠ΅Ρ‚Π΅Π½Ρ†ΠΈΠΈ ΠΈ ΠΌΠΎΠΆΠ΅Ρ‚ ΡΠ²Π»ΡΡ‚ΡŒΡΡ ΠΏΡ€Π΅Π΄ΠΌΠ΅Ρ‚ΠΎΠΌ Π΄Π΅ΠΉΡΡ‚Π²ΡƒΡŽΡ‰Π΅Π³ΠΎ Π½Π°Ρ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ
Π·Π°ΠΊΠΎΠ½ΠΎΠ΄Π°Ρ‚Π΅Π»ΡŒΡΡ‚Π²Π° ΠΈ Ρ€Π΅Π³Π»Π°ΠΌΠ΅Π½Ρ‚ΠΎΠ². Π’Π°ΠΊΠΈΠ΅ испытания Π΄ΠΎΠ»ΠΆΠ΅Π½ Π²Ρ‹ΠΏΠΎΠ»Π½ΡΡ‚ΡŒ Ρ‚ΠΎΠ»ΡŒΠΊΠΎ пСрсонал,
ΠΈΠΌΠ΅ΡŽΡ‰ΠΈΠΉ ΠΎΠΏΡ‹Ρ‚ Ρ€Π°Π±ΠΎΡ‚Ρ‹ с микробиологичСскими ΠΌΠ΅Ρ‚ΠΎΠ΄Π°ΠΌΠΈ. ΠŸΡ€Π°Π²ΠΈΠ»Π° Π΄Π΅Π·ΠΈΠ½Ρ„Π΅ΠΊΡ†ΠΈΠΈ, стСрилизации
ΠΈ Π»ΠΈΡ‡Π½ΠΎΠΉ Π³ΠΈΠ³ΠΈΠ΅Π½Ρ‹ Π΄ΠΎΠ»ΠΆΠ½Ρ‹ строго ΡΠΎΠ±Π»ΡŽΠ΄Π°Ρ‚ΡŒΡΡ.
ΠŸΠ΅Ρ€ΡΠΎΠ½Π°Π»Ρƒ рСкомСндуСтся ΠΎΠ±Ρ€Π°Ρ‚ΠΈΡ‚ΡŒ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ Π½Π° ΠΌΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹Π΅ стандарты
IEC 60068-2-10:2005, ΠŸΡ€ΠΈΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅ A β€œΠžΠΏΠ°ΡΠ½ΠΎΡΡ‚ΡŒ для пСрсонала”, ΠΈ ISO 7218, ΠœΠΈΠΊΡ€ΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡ
ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ΠΎΠ² питания ΠΈ ΠΊΠΎΡ€ΠΌΠΎΠ² для ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…. ΠžΠ±Ρ‰ΠΈΠ΅ трСбования ΠΈ ΠΏΡ€Π°Π²ΠΈΠ»Π°
микробиологичСских исслСдований.
1 ΠžΠ±Π»Π°ΡΡ‚ΡŒ примСнСния
Настоящий ΠΌΠ΅ΠΆΠ΄ΡƒΠ½Π°Ρ€ΠΎΠ΄Π½Ρ‹ΠΉ стандарт устанавливаСт ΠΌΠ΅Ρ‚ΠΎΠ΄ опрСдСлСния эффСктивности
фунгистатичСских соСдинСний ΠΏΡ€ΠΈ Π·Π°Ρ‰ΠΈΡ‚Π΅ Ρ‡ΡƒΠ²ΡΡ‚Π²ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… ΠΈΠ½Π³Ρ€Π΅Π΄ΠΈΠ΅Π½Ρ‚ΠΎΠ², Π½Π°ΠΏΡ€ΠΈΠΌΠ΅Ρ€, пластификаторов,
стабилизаторов ΠΈ Ρ‚.Π΄. Π² составС пластмассы. ΠœΠ΅Ρ‚ΠΎΠ΄ дСмонстрируСт, Ρ…ΠΎΡ€ΠΎΡˆΠΎ ΠΈΠ»ΠΈ ΠΏΠ»ΠΎΡ…ΠΎ Π·Π°Ρ‰ΠΈΡ‰Π΅Π½
пластмассовый ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ ΠΎΡ‚ воздСйствия плСсСни.
ΠžΡ†Π΅Π½ΠΊΡƒ Π²Ρ‹ΠΏΠΎΠ»Π½ΡΡŽΡ‚ посрСдством Π²ΠΈΠ·ΡƒΠ°Π»ΡŒΠ½ΠΎΠ³ΠΎ обслСдования.
Π”Π°Π½Π½ΠΎΠ΅ испытаниС ΠΏΡ€ΠΈΠΌΠ΅Π½ΠΈΠΌΠΎ ΠΊ Π»ΡŽΠ±ΠΎΠΌΡƒ издСлию ΠΈΠ· пластмассы Π² Ρ„ΠΎΡ€ΠΌΠ΅ ΠΏΠ»Π΅Π½ΠΊΠΈ ΠΈΠ»ΠΈ листов Π½Π΅ Ρ‚ΠΎΠ»Ρ‰Π΅
10 ΠΌΠΌ. ΠšΡ€ΠΎΠΌΠ΅ Ρ‚ΠΎΠ³ΠΎ, пористыС ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹, Ρ‚Π°ΠΊΠΈΠ΅ ΠΊΠ°ΠΊ пСнопласты, ΠΌΠΎΠΆΠ½ΠΎ ΠΈΡΠΏΡ‹Ρ‚Ρ‹Π²Π°Ρ‚ΡŒ, ΠΏΡ€ΠΈ условии, Ρ‡Ρ‚ΠΎ
ΠΎΠ½ΠΈ исполнСны Π² ΡƒΠΊΠ°Π·Π°Π½Π½ΠΎΠΉ Π²Ρ‹ΡˆΠ΅ Ρ„ΠΎΡ€ΠΌΠ΅.
Минимальная диффузия Ρ„ΡƒΠ½Π³ΠΈΡ†ΠΈΠ΄ΠΎΠ², ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΠΌΠΈΠ³Ρ€ΠΈΡ€ΡƒΡŽΡ‚ ΠΈΠ· ΠΌΠ°Ρ‚Ρ€ΠΈΡ†Ρ‹, Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΠΎ для Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°.
А ΠΏΡ€ΠΎΡ‚ΠΈΠ²ΠΎΠΏΠΎΠ»ΠΎΠΆΠ½ΠΎΡΡ‚ΡŒ ISO 846, испытуСмыС ΠΏΠ»Π΅Π½ΠΊΠΈ Π½Π΅ ΠΎΠΏΡ€Ρ‹ΡΠΊΠΈΠ²Π°ΡŽΡ‚ взвСсью спор плСсСни, Π°
ΠΏΠΎΠΊΡ€Ρ‹Π²Π°ΡŽΡ‚ слоСм ΠΈΡΠΏΡ‹Ρ‚Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΠ³ΠΎ Π°Π³Π°Ρ€Π°, содСрТащСго споры. ΠžΠ±Π½Π°Ρ€ΡƒΠΆΠ΅Π½ΠΎ, Ρ‡Ρ‚ΠΎ это Π²Π΅Π΄Π΅Ρ‚ ΠΊ Π»ΡƒΡ‡ΡˆΠ΅ΠΌΡƒ
Ρ€Π°ΡΠΏΡ€Π΅Π΄Π΅Π»Π΅Π½ΠΈΡŽ спор, Π° Ρ‚Π°ΠΊΠΆΠ΅ обСспСчиваСт Ρ…ΠΎΡ€ΠΎΡˆΠ΅ΠΉ ΠΏΠΎΠ΄Π°Ρ‡Π΅ΠΉ Π²Π»Π°Π³ΠΈ, Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΠΎΠΉ для прорастания
спор Π½Π° повСрхности пластмассы, которая ΠΎΠ±Ρ‹Ρ‡Π½ΠΎ являСтся Π³ΠΈΠ΄Ρ€ΠΎΡ„ΠΎΠ±Π½ΠΎΠΉ.
2 НормативныС ссылки
Π‘Π»Π΅Π΄ΡƒΡŽΡ‰ΠΈΠ΅ справочныС Π΄ΠΎΠΊΡƒΠΌΠ΅Π½Ρ‚Ρ‹ Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΡ‹ для примСнСния Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π΄ΠΎΠΊΡƒΠΌΠ΅Π½Ρ‚Π°. Для ТСстких
ссылок ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΠ΅Ρ‚ΡΡ Ρ‚ΠΎΠ»ΡŒΠΊΠΎ ΡƒΠΊΠ°Π·Π°Π½Π½ΠΎΠ΅ ΠΈΠ·Π΄Π°Π½ΠΈΠ΅. Для ΠΏΠ»Π°Π²Π°ΡŽΡ‰ΠΈΡ… ссылок ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΠ΅Ρ‚ΡΡ самоС
послСднСС ΠΈΠ·Π΄Π°Π½ΠΈΠ΅ основного Π΄ΠΎΠΊΡƒΠΌΠ΅Π½Ρ‚Π° (Π²ΠΊΠ»ΡŽΡ‡Π°Ρ всС измСнСния).
ISO 291:2008, ΠŸΠ»Π°ΡΡ‚ΠΌΠ°ΡΡΡ‹. Π‘Ρ‚Π°Π½Π΄Π°Ρ€Ρ‚Π½Ρ‹Π΅ атмосфСры для кондиционирования ΠΈ испытания
3 Π’Π΅Ρ€ΠΌΠΈΠ½Ρ‹ ΠΈ опрСдСлСния
ΠŸΡ€ΠΈΠΌΠ΅Π½ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ ΠΊ Π΄Π°Π½Π½ΠΎΠΌΡƒ Π΄ΠΎΠΊΡƒΠΌΠ΅Π½Ρ‚Ρƒ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΡŽΡ‚ΡΡ ΡΠ»Π΅Π΄ΡƒΡŽΡ‰ΠΈΠ΅ Ρ‚Π΅Ρ€ΠΌΠΈΠ½Ρ‹ ΠΈ опрСдСлСния.
3.1
пластмассы, Ρ‡ΡƒΠ²ΡΡ‚Π²ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅ ΠΊ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ плСсСни
plastic susceptible to fungal attack
пластмассовый ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π», ΠΊΠΎΡ‚ΠΎΡ€Ρ‹ΠΉ содСрТит Π² своСм составС ΠΎΠ΄Π½ΠΎ ΠΈΠ»ΠΈ нСсколько ΠΏΠΈΡ‚Π°Ρ‚Π΅Π»ΡŒΠ½Ρ‹Ρ…
вСщСств/. ΠΏΠΎΠ΄Π΄Π΅Ρ€ΠΆΠΈΠ²Π°ΡŽΡ‰ΠΈΡ… рост плСсСни
3.2
Ρ„ΡƒΠ½Π³ΠΈΡ†ΠΈΠ΄
fungistat
соСдинСниС, ΠΊΠΎΡ‚ΠΎΡ€ΠΎΠ΅ ΠΏΡ€Π΅Π΄ΠΎΡ‚Π²Ρ€Π°Ρ‰Π°Π΅Ρ‚ рост плСсСни Π½Π° ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π΅, ΠΎΠ±Ρ‹Ρ‡Π½ΠΎ Ρ‡ΡƒΠ²ΡΡ‚Π²ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΌ ΠΊ
Π²ΠΎΠ·Π΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ плСсСни
4 Π‘ΡƒΡ‰Π½ΠΎΡΡ‚ΡŒ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°
Π˜ΡΠΏΡ‹Ρ‚ΡƒΠ΅ΠΌΡ‹Π΅ ΠΎΠ±Ρ€Π°Π·Ρ†Ρ‹ ΠΏΠΎΠ΄Π²Π΅Ρ€Π³Π°ΡŽΡ‚ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ суспСнзии ΡΠΌΠ΅ΡˆΠ°Π½Π½Ρ‹Ρ… спор плСсСни. Π‘ΠΏΠΎΡ€Ρ‹ наносят Π½Π°
ΠΏΠΎΠ²Π΅Ρ€Ρ…Π½ΠΎΡΡ‚ΡŒ испытуСмого ΠΎΠ±Ρ€Π°Π·Ρ†Π° Ρ‚ΠΎΠ½ΠΊΠΈΠΌ слоСм Π½Π° Π°Π³Π°Ρ€ΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠΉ срСдС Π±Π΅Π· добавлСния источника
ΡƒΠ³Π»Π΅Ρ€ΠΎΠ΄Π°. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ достигаСтся Ρ€Π°Π²Π½ΠΎΠΌΠ΅Ρ€Π½ΠΎ распрСдСлСниС спор, Π° Ρ‚Π°ΠΊΠΆΠ΅ ΠΎΠΏΡ‚ΠΈΠΌΠ°Π»ΡŒΠ½Ρ‹ΠΉ ΠΏΠΎΠ΄Π²ΠΎΠ΄
Π²Π»Π°Π³ΠΈ.
ΠžΡ‚ΡΡƒΡ‚ΡΡ‚Π²ΠΈΠ΅ фунгистатичСских вСщСств Π² пластмассовом ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π΅ ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ ΠΏΡ€ΠΎΡ€Π°ΡΡ‚Π°Π½ΠΈΡŽ спор
плСсСни ΠΈ Π½Π°Ρ‡Π°Π»ΡŒΠ½ΠΎΠΌΡƒ росту. Если ΠΈΠ½Π³Ρ€Π΅Π΄ΠΈΠ΅Π½Ρ‚Ρ‹ Π² ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π΅ Ρ‡ΡƒΠ²ΡΡ‚Π²ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ ΠΊ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ плСсСни ΠΈ
Π² состав Π΅Π³ΠΎ Π½Π΅ Π²Ρ…ΠΎΠ΄ΠΈΡ‚ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΠ³ΠΎ Ρ„ΡƒΠ½Π³ΠΈΡ†ΠΈΠ΄Π° происходит дальнСйший рост ΠΈ спорообразованиС ΠΏΠΎ
всСму испытуСмому ΠΎΠ±Ρ€Π°Π·Ρ†Ρƒ.
ΠŸΡ€ΠΈΡΡƒΡ‚ΡΡ‚Π²ΠΈΠ΅ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΠ³ΠΎ Ρ„ΡƒΠ½Π³ΠΈΡ†ΠΈΠ΄Π° Π² ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Π΅ ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ подавлСнию прорастания спор ΠΈ
Π½Π°Ρ‡Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ роста плСсСни ΠΏΠΎ всСму испытуСмому ΠΎΠ±Ρ€Π°Π·Ρ†Ρƒ. ЀунгистатичСскиС вСщСства ΠΌΠΎΠ³ΡƒΡ‚
ΠΌΠΈΠ³Ρ€ΠΈΡ€ΠΎΠ²Π°Ρ‚ΡŒ Π² Π°Π³Π°Ρ€ ΠΏΠΎ испытуСмому ΠΎΠ±Ρ€Π°Π·Ρ†Ρƒ, подавляя ΠΏΡ€ΠΈ этом прорастаниС ΠΈ формируя
ΡƒΠ²Π΅Π»ΠΈΡ‡ΠΈΠ²Π°ΡŽΡ‰ΡƒΡŽΡΡ Π·ΠΎΠ½Ρƒ ингибирования.
Π₯отя это ΠΈ Π½Π΅ относится ΠΊ ΠΈΠ½Ρ‚Π΅Ρ€ΠΏΡ€Π΅Ρ‚Π°Ρ†ΠΈΠΈ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚ΠΎΠ², Π·ΠΎΠ½Π° ингибирования ΠΌΠΎΠΆΠ΅Ρ‚ ΡΠ²Π»ΡΡ‚ΡŒΡΡ
ΠΏΠΎΠΊΠ°Π·Π°Ρ‚Π΅Π»Π΅ΠΌ повСдСния Π°Π½Π°Π»ΠΈΠ·ΠΈΡ€ΡƒΠ΅ΠΌΠΎΠ³ΠΎ Ρ„ΡƒΠ½Π³ΠΈΡ†ΠΈΠ΄Π°.
5 Аппаратура ΠΈ ΠΌΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹
5.1 Аппаратура
Π‘Ρ‚Π΅Ρ€ΠΈΠ»ΠΈΠ·ΡƒΡŽΡ‚ всю ΡΡ‚Π΅ΠΊΠ»ΡΠ½Π½ΡƒΡŽ посуду ΠΈ части ΠΎΡΡ‚Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ оборудования, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ Π±ΡƒΠ΄ΡƒΡ‚ ΡΠΎΠΏΡ€ΠΈΠΊΠ°ΡΠ°Ρ‚ΡŒΡΡ с
ΠΏΠΈΡ‚Π°Ρ‚Π΅Π»ΡŒΠ½ΠΎΠΉ срСдой ΠΈ/ΠΈΠ»ΠΈ Ρ€Π΅Π°ΠΊΡ‚ΠΈΠ²Π°ΠΌΠΈ (Π·Π° ΠΈΡΠΊΠ»ΡŽΡ‡Π΅Π½ΠΈΠ΅ΠΌ Ρ‚Π΅Ρ…, ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Π΅ ΠΏΠΎΡΡ‚Π°Π²Π»ΡΡŽΡ‚ΡΡ ΡΡ‚Π΅Ρ€ΠΈΠ»ΡŒΠ½Ρ‹ΠΌΠΈ)
ΠΎΠ΄Π½ΠΈΠΌ ΠΈΠ· ΡΠ»Π΅Π΄ΡƒΡŽΡ‰ΠΈΡ… ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ²:
ΠœΠ΅Ρ‚ΠΎΠ΄ A: Π² Π°Π²Ρ‚ΠΎΠΊΠ»Π°Π²Π΅ (см. 5.1.2) ΠΏΡ€ΠΈ Ρ‚Π΅ΠΌΠΏΠ΅Ρ€Π°Ρ‚ΡƒΡ€Π΅ 121 Β°C Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ 15 ΠΌΠΈΠ½;
ΠΌΠ΅Ρ‚ΠΎΠ΄ B: ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΡŽΡ‚ стСрилизатор сухим ΠΆΠ°Ρ€ΠΎΠΌ (см. 5.1.2) ΠΏΡ€ΠΈ Ρ‚Π΅ΠΌΠΏΠ΅Ρ€Π°Ρ‚ΡƒΡ€Π΅ 180 Β°C Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ Π½Π΅
ΠΌΠ΅Π½Π΅Π΅ 30 ΠΌΠΈΠ½, ΠΏΡ€ΠΈ Ρ‚Π΅ΠΌΠΏΠ΅Ρ€Π°Ρ‚ΡƒΡ€Π΅ 170 Β°C Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ 1 Ρ‡, ΠΈΠ»ΠΈ ΠΏΡ€ΠΈ Ρ‚Π΅ΠΌΠΏΠ΅Ρ€Π°Ρ‚ΡƒΡ€Π΅ 160 Β°C Π²
Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ Π½Π΅ ΠΌΠ΅Π½Π΅Π΅ 2 Ρ‡;
ΠΌΠ΅Ρ‚ΠΎΠ΄ C: ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΡŽΡ‚ систСму Ρ„ΠΈΠ»ΡŒΡ‚Ρ€ΠΎΠ²Π°Π½ΠΈΡ Ρ‡Π΅Ρ€Π΅Π· ΠΌΠ΅ΠΌΠ±Ρ€Π°Π½Π½Ρ‹ΠΉ Ρ„ΠΈΠ»ΡŒΡ‚Ρ€ с Ρ€Π°Π·ΠΌΠ΅Ρ€ΠΎΠΌ ΠΏΠΎΡ€ 0,45 ΠΌΠΊΠΌ.
5.1.1 Π˜Π½ΠΊΡƒΠ±Π°Ρ‚ΠΎΡ€ (тСрмостат), ΠΏΠΎΠ΄Π΄Π΅Ρ€ΠΆΠΈΠ²Π°Π΅ΠΌΡ‹ΠΉ ΠΏΡ€ΠΈ Ρ‚Π΅ΠΌΠΏΠ΅Ρ€Π°Ρ‚ΡƒΡ€Π΅ 24 Β°C Β± 1 Β°C.
5.1.2 ΠžΠ±ΠΎΡ€ΡƒΠ΄ΠΎΠ²Π°Π½ΠΈΠ΅ для стСрилизации:
5.1.2.1 Для стСрилизации Π²Π»Π°ΠΆΠ½
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