ISO 19056-2:2019

INTERNATIONAL ISO
STANDARD 19056-2
First edition
2019-11
Microscopes — Definition and
measurement of illumination
properties —
Part 2:
Illumination properties related to the
colour in bright field microscopy
Microscopes — Définition et mesurage des propriétés d'éclairage —
Partie 2: Propriétés d'illumination liées à la couleur en microscopie à
champ lumineux
Reference number
©
ISO 2019
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved

Contents  Page
Foreword .iv
1 Scope . 1
2  Normative references . 1
3  Terms and definitions . 1
4  Measurands . 1
4.1 General . 1
4.2 Spectral measurement. 1
4.3 Chromaticity . 2
4.4 Correlated colour temperature, T .
cp 3
4.4.1 General. 3
4.4.2 Correlated colour temperature using the CIE 1960 UCS chromaticity
diagram and its procedure . 3
4.4.3 Correlated colour temperature using (x, y) chromaticity coordinates . 3
5  Measurement procedures . 3
5.1 General . 3
5.2 Measurement environment. 4
5.3 Integrating sphere . 4
5.4 Microscope settings . 4
5.4.1 Diaphragm settings . . 4
5.4.2 Adjustment of samples and focusing for diaphragms . 4
5.4.3 Other combinations . 4
5.4.4 Adjustment of light source . 4
5.5 Stability of measurement . 4
6  Information provided to the user . 5
6.1 General . 5
6.2 Information of chromaticity and correlated colour temperature . 5
6.3 Additional information . 6
Bibliography . 8
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 172, Optics and photonics, Subcommittee
SC 5, Microscopes and endoscopes.
A list of all parts in the ISO 19056 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2019 – All rights reserved

INTERNATIONAL STANDARD ISO 19056-2:2019(E)
Microscopes — Definition and measurement of
illumination properties —
Part 2:
Illumination properties related to the colour in bright field
microscopy
1 Scope
This document specifies measurands and measurement procedures of colour properties for bright field
microscopy with transmitted light illumination. These measurements are defined in image planes or
intermediate image planes.
This document also specifies how the information is provided to the user.
2  Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 11664-1:2019, Colorimetry — Part 1: CIE standard colorimetric observers
ISO 11664-3, Colorimetry — Part 3: CIE tristimulus values
3  Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at http:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
4  Measurands
4.1  General
Since various light sources such as halogen lamps, tungsten lamps, LEDs, and OLEDs are used for
bright field microscopy, it is essential to understand the difference in colour properties due to the light
sources. This applies for different applications and various types of instruments.
4.2  Spectral measurement
The spectral radiant flux (W) shall be measured by placing an instrument equipped with an integrating
sphere and an optical spectrometer function in an image plane or in an intermediate image plane. The
measurements shall be performed in the measurement spectral range from 380 nm to 780 nm with
5 nm intervals or less.
4.3  Chromaticity
The chromaticity coordinates (x, y) shall be calculated from the
...