ISO 24066:2026

FINAL DRAFT
International
Standard
ISO/FDIS 24066
ISO/TC 249/SC 1
Traditional Chinese medicine —
Secretariat: SAC
Euodia (syn. Evodia or Tetradium)
Voting begins on:
fruit
2025-11-13
Voting terminates on:
2026-01-08
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
ISO/FDIS 24066:2025(en) © ISO 2025

FINAL DRAFT
ISO/FDIS 24066:2025(en)
International
Standard
ISO/FDIS 24066
ISO/TC 249/SC 1
Traditional Chinese medicine —
Secretariat: SAC
Euodia (syn. Evodia or
Voting begins on:
Tetradium) fruit
Voting terminates on:
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2025
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO­
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ISO/FDIS 24066:2025(en) © ISO 2025

ii
ISO/FDIS 24066:2025(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Description . . 2
5 Quality and safety requirements and recommendations . 2
5.1 General characteristics .2
5.2 Macroscopic characteristics .2
5.3 Microscopic characteristics .2
5.4 Thin-layer chromatography (TLC) .3
5.5 Moisture .3
5.6 Total ash .3
5.7 Ethanol-soluble extractives .3
5.8 Marker compound(s) .3
5.9 Heavy metals .4
5.10 Pesticide residues .4
5.11 Sulfur dioxide . .4
6 Sampling . 4
7 Test methods . 4
7.1 Macroscopic identification .4
7.2 Microscopic identification .4
7.3 Thin-layer chromatographic identification .4
7.4 Determination of moisture .4
7.5 Determination of total ash .4
7.6 Determination of extractives .4
7.7 Determination of evodiamine, rutecarpine and limonin .4
7.8 Determination of heavy metals .4
7.9 Determination of pesticide residues .4
7.10 Determination of sulfur dioxide .5
8 Test report . 5
9 Packaging, storage and transportation . 5
10 Marking and labelling . 5
Annex A (informative) Thin-layer chromatographic (TLC) identification . 6
Annex B (informative) Determination of evodiamine, rutecarpine and limonin . 8
Annex C (informative) Reference information of national and regional requirements .11
Bibliography .13

iii
ISO/FDIS 24066:2025(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 249, Traditional medicine, Subcommittee SC 1,
Traditional Chinese medicine.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
ISO/FDIS 24066:2025(en)
Introduction
Euodia fruit is known as wúzhūyú (吴茱萸) in Chinese. It is a medicinal herb which has been widely used as
functional food and crude drug to dissipate cold, relieve pain, downbear counterflow, stop vomiting, assist
yang and stop diarrhoea in Asian countries for thousands of years.
The products of Euodia fruit are sold all over the world. Euodia fruit has been recorded by the Chinese
pharmacopoeia, Hong Kong Chinese materia medica standards, the European pharmacopoeia, the Japanese
pharmacopoeia and the Korean pharmacopoeia. However, the requirements and test methods of Euodia fruit
in these national and regional standards are varied and can cause barriers in international trade.
Factors including producing areas, processing, packaging and storage conditions can affect the quality of
Euodia fruit. The quality of Euodia fruit in the market can be unstable. In addition, due to its demand in the
global market, trade in Euodia fruit can be complicated by adulteration and substitution issues.
As an important herb in traditional Chinese medicine, Euodia fruit is ranked the seventeenth in the priority
list of single herbal medicines for developing standards in ISO/TR 23975. Thus, it is essential to develop an
International Standard for Euodia fruit to maintain consistent quality of Euodia fruit, ensure the safe use of
this herb and promote international trade.
As national implementation can differ, national standards bodies are invited to modify the values given in
5.6 in their national standards. Examples of national and regional values are given in Annex C.

v
FINAL DRAFT International Standard ISO/FDIS 24066:2025(en)
Traditional Chinese medicine — Euodia (syn. Evodia or
Tetradium) fruit
1 Scope
This document specifies the quality, safety requirements and test methods for Euodia fruit.
It is applicable to Euodia fruit that is sold and used as natural medicines in international trade, including
Chinese materia medica (whole medicinal materials) and decoction pieces.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 18664, Traditional Chinese Medicine — Determination of heavy metals in herbal medicines used in
Traditional Chinese Medicine
ISO/TS 21310, Traditional Chinese medicine — Microscopic examination of medicinal herbs
ISO 21371, Traditional Chinese medicine — Labelling requirements of products intended for oral or topical use
ISO 22217, Traditional Chinese medicine —Storage requirements for raw materials and decoction pieces
ISO 22258, Traditional Chinese medicine — Determination of pesticide residues in natural products by gas
chromatography
ISO 22590, Traditional Chinese medicine — Determination of sulfur dioxide in natural products by titration
ISO 23723, Traditional Chinese medicine — General requirements for herbal raw material and materia medica
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
Euodia fruit
dried and nearly ripe fruit of Euodia (Evodia) rutaecarpa (Juss.) Benth., Euodia (Evodia) rutaecarpa (Juss.)
Benth. var. officinalis (Dode) Huang or Euodia (Evodia) rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang
[Tetradium ruticarpum (A. Juss.) T. G. Hartley]
3.2
marker compound
chemical constituent within a medicinal herb that can be used to verify its quality
Note 1 to entry: Marker compounds are usual
...

ISO/FDIS 24066:2025(en)
ISO/TC 249/SC 1/WG 1
Secretariat: SAC
Date: 2025-09-0510-29
Traditional Chinese medicine— — Euodia (syn. Evodia or Tetradium)
fruit
FDIS stage
ISO/DISFDIS 24066:2025(en)
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: + 41 22 749 01 11
Fax: +41 22 749 09 47
EmailE-mail: copyright@iso.org
Website: www.iso.org
Published in Switzerland
© ISO #### 2025 – All rights reserved
ii
ISO/FDIS 24066:2025(en)
Contents
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Description . 2
5 Quality and safety requirements and recommendations . 3
5.1 General characteristics . 3
5.2 Macroscopic characteristics . 3
5.3 Microscopic characteristics. 3
5.4 Thin-layer chromatography (TLC) . 5
5.5 Moisture . 5
5.6 Total ash . 5
5.7 Ethanol-soluble extractives. 5
5.8 Marker compound(s) . 5
5.9 Heavy metals . 5
5.10 Pesticide residues . 5
5.11 Sulfur dioxide . 5
6 Sampling . 5
7 Test methods . 5
7.1 Macroscopic identification . 5
7.2 Microscopic identification . 6
7.3 Thin-layer chromatographic identification . 6
7.4 Determination of moisture . 6
7.5 Determination of total ash . 6
7.6 Determination of extractives . 6
7.7 Determination of evodiamine, rutecarpine and limonin . 6
7.8 Determination of heavy metals. 6
7.9 Determination of pesticide residues . 6
7.10 Determination of sulfur dioxide . 6
8 Test report . 6
9 Packaging, storage and transportation . 7
10 Marking and labelling . 7
Annex A (informative) Thin-layer chromatographic (TLC) identification . 8
Annex B (informative) Determination of evodiamine, rutecarpine and limonin . 11
Annex C (informative) Reference information of national and regional requirements . 16
Bibliography . 18

iii
ISO/DISFDIS 24066:2025(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types of
ISO document should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent rights
in respect thereof. As of the date of publication of this document, ISO had not received notice of (a) patent(s)
which may be required to implement this document. However, implementers are cautioned that this may not
represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 249, Traditional medicine, Subcommittee SC 1,
Traditional Chinese medicine.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
© ISO #### 2025 – All rights reserved
iv
ISO/FDIS 24066:2025(en)
Introduction
Euodia fruit is the dried and nearly ripe fruit of Euodia (Evodia) rutaecarpa (Juss.) Benth., Euodia (Evodia)
rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang or Euodia (Evodia) rutaecarpa (Juss.) Benth. var.
bodinieri (Dode) Huang (Tetradium ruticarpum (A. Juss.) T. G. Hartley) andEuodia fruit is known as wúzhūyú
(吴茱萸) in Chinese. It is a medicinal herb which has been widely used as functional food and crude drug to
dissipate cold, relieve pain, downbear counterflow, stop vomiting, assist yang and stop diarrheadiarrhoea in
Asian countries for thousands of years.
The products of Euodia fruit are sold all over the world. Euodia fruit has been recorded by the Chinese
pharmacopoeia, Hong Kong Chinese materia medica standards, the European pharmacopoeia, the Japanese
pharmacopoeia and the Korean pharmacopoeia. However, the requirements and test methods of Euodia fruit
in these national and regional standards are varied and maycan cause barriers in international trade.
Factors including producing areas, processing, packaging and storage conditions maycan affect the quality of
Euodia fruit. The quality of Euodia fruit in the market maycan be unstable. In addition, due to its demand in
the global market, trade in Euodia fruit maycan be complicated by adulteration, and substitution issues.
As an important herb in traditional Chinese medicine, Euodia fruit is ranked the seventeenth in the ISO/TR
23975: 2025 Traditional Chinese medicine — Prioritypriority list of single herbal medicines for developing
standards. in ISO/TR 23975. Thus, it is essential to develop the international standardan International
Standard for Euodia fruit to ensure the consistency in maintain consistent quality of Euodia fruit, which is
important inensure the safe use of this herb and also promote the international trade.
As national implementation maycan differ, national standards bodies are invited to modify the values given in
5.65.6 in their national standards. Examples of national and regional values are given in Annex CAnnex C.
v
ISO/FDIS 24066:2025(en)
FINAL DRAFT International Standard

Traditional Chinese medicine — — Euodia (syn. Evodia or Tetradium)
fruit
1 Scope
This document specifies the quality, safety requirements and test methods for Euodia fruit that is derived from
the fruit of Euodia (Evodia) rutaecarpa (Juss.) Benth., Euodia (Evodia) rutaecarpa (Juss.) Benth. var. officinalis
(Dode) Huang or Euodia (Evodia) rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang (Tetradium ruticarpum
(A. Juss.) T. G. Hartley).
It is applicable to Euodia fruit that areis sold and used as natural medicines in international trade, including
Chinese materia medica (whole medicinal materials) and decoction pieces.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 18664, Traditional Chinese Medicine — Determination of heavy metals in herbal medicines used in
Traditional Chinese Medicine
ISO/TS 21310, Traditional Chinese medicine -— Microscopic examination of medicinal herbs
ISO 21371, Traditional Chinese medicine — Labelling requirements of products intended for oral or topical use
ISO 22217, Traditional Chinese medicine —Storage requirements for raw materials and decoction pieces
ISO 22258, Traditional Chinese medicine — Determination of pesticide residues in natural products by gas
chromatography
ISO 22590, Traditional Chinese medicine — Determination of sulfur dioxide in natural products by titration
ISO 23723, Traditional Chinese medicine — General requirements for herbal raw material and materia medica
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— — ISO Online browsing platform: available at https://www.iso.org/obp
— — IEC Electropedia: available at https://www.electropedia.org/
ISO/DISFDIS 24066:2025(en)
3.1 3.1
Euodia fruit
dried and nearly ripe fruit of Euodia (Evodia) rutaecarpa (Juss.) Benth., Euodia (Evodia) rutaecarpa (Juss.)
Benth. var. officinalis (Dode) Huang or Euodia (Evodia) rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang
([Tetradium ruticarpum (A. Juss.) T. G. Hartley)]
3.2 3.2
marker compound
chemical constituent within a medicinal herb that can be used to verify its quality
Note 1 to entry: Usually Marker compounds are usually described as active ingredients or chemicals that confirm the
correct botanical identity of the starting material.
Note 2 to entry: There can be one or more marker compounds for a medicinal herb.
4 Description
4 Euodia fruit is shown in Figure 1Descriptions
Euodia fruit is the dried and nearly ripe fruit of Euodia (Evodia) rutaecarpa (Juss.) Benth., Euodia (Evodia)
rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang or Euodia (Evodia) rutaecarpa (Juss.) Benth. var.
bodinieri (Dode) Huang (Tetradium ruticarpum (A. Juss.) T. G. Hartley), as shown in Figure 1.

.
Key
protrudings
2a) Side view oil dotsb) Vertical view
3 cleft
4 stalk
© ISO #### 2025 – All rights reserved
ISO/FDIS 24066:2025(en)
Key
1 protrudings
2 oil dots
3 cleft
4 stalk
Figure 1 — Structure of Euodia fruit
5 Quality and safety requirements and recommendations
5.1 General characteristics
The following requirements shall be met before sampling:.
a) a) Euodia fruit shall be clean and free from foreign matter;.
b) b) The presence of living insects, moulds and external contaminants which are visible to the
naked eye shall not be permitted.
5.2 Macroscopic characteristics
SpheroidalEuodia fruit is spheroidal or slightly flattened-pentagon spheroidal, 2 mm to 5 mm in diameter.
ExternallyIt is externally dark yellowish-green to brown, rough, with numerous spotted protrudings or
depressed oil dots. A pentagon-stellate cleft is present at the apex and a yellow-tomentose fruit stalk at the
base. Texture,The texture is hard and fragile. TransverseThe transverse section showingshows 5-locular
ovary, each locule possessing 1 yellowish seed. Odour,The odour is strong and aromatic;. The taste, is pungent
and bitter.
5.3 Microscopic characteristics
a) a) Transverse section, ( [see Figure 2 aFigure 2a):)]: 1 row epidermis cells of exocarp (1); the oil
chamber (2) is large, with a diameter of 45 µm to 460 µm, distributed in mesocarp (3); the vascular
bundles (4) are distributed near the endocarp (5); clusters of calcium oxalate (6) are distributed in
mesocarp parenchyma cells, especially near endocarp composed of 4- to 5 rows of oblong parenchyma
cells; sometimes stone cells (7) can be seen.
b) b) Powder, ( [see Figure 2 bFigure 2b):)]: brown; 1- to 6 celled non-glandular hairs (8), 140- µm
to 350 µm long, warts obvious, some lumina containing brownish-yellow to brownish-red contents;
glandular hairs (9) with a 7- to 14 celled head, elliptical, usually containing yellowish-brown contents,
and with a 2- to 5 celled stalk; clusters of calcium oxalate (6) frequently, 10 µm to 25 µm in diameter;
stone cells (7) subrounded or rectangular, 35 µm to 70 µ
...