ISO 13730:1996

IS0
INTERNATIONAL
13730
STANDARD
First edition
1996-12-15
Meat and meat products - Determination
of total phosphorus content -
Spectrometric method
Viande et produits 2 base de viande - Dktermination de la teneur en
M6 thode spectromk trique
phosphore total -
Reference number
IS0 13730: 1996(E)

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ISO13730:1996(E)
Foreword
IS0 (the international Organization for Standardization) is a worldwide
federation of national standards bodies (IS0 member bodies). The work of
preparing International Standards is normally carried out through IS0
technical committees. Each member body interested in a subject for which
a technical committee has been established has the right to be represented
on that committee. International organizations, governmental and non-
governmental, in liaison with ISO, also take part in the work. IS0
collaborates closely with the International Electrotechnical Commission
(IEC) on all matters of electrotechnical standardization.
Draft International Standards adopted by the technical committees are
circulated to the member bodies for voting. Publication as an International
Standard requires approval by at least 75 % of the member bodies casting
a vote.
International Standard IS0 13730 was prepared by Technical Committee
ISOTTC 34, Agricultural food products, Subcommittee SC 6, Meat and meat
products.
Annex A of this International Standard is for information only.
0 IS0 1996
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced
or utilized in any form or by any means, electronic or mechanical, including protocopying and
microfilm, without permission in writing from the publisher.
International Organization for Standardization
Case postale 56 l CH-1211 Geneve 20 l Switzerland
Internet: central @ isocs.iso.ch
x.400: c=ch; a=400net; p=iso; o=isocs; s=central
Printed in Switzerland
ii

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IS0 13730: 1996(E)
INTERNATIONAL STANDARD 0 IS0
Meat and meat products - Determination of total
phosphorus content - Spectrometric method
1 Scope
This International Standard specifies a method for the determination of the total phosphorus content of all kinds of
meat and meat products, including poultry. The precision results quoted in this method relate only to processed
sausages.
2 Normative reference
-The following standard contains provisions which, through reference in this text, constitute provisions of this
International Standard. At the time of publication, the edition indicated was valid. All standards are subject to
revision, and parties to agreements based on this International Standard are encouraged to investigate the
possibility of applying the most recent edition of the standard indicated below. Members of IEC and IS0 maintain
registers of currently valid International Standards.
IS0 936:-1>, Meat and meat products - Determination of ash.
3 Definition
For the purposes of this International Standard, the following definition applies.
31 total phosphorus content of meat and meat products: Mass of phosphorus pentoxide determined
by the procedure specified in this International Standard, expressed as a percentage of the mass of the test portion.
4 Principle
Drying of the test portion and incineration of the residue. After cooling, hydrolysis of the ash with nitric acid.
Filtration and dilution followed by the formation of a yellow compound with a mixture of ammonium
monovanadate and ammonium heptamolybdate. Photometric measurement at a wavelength of 430 nm.
1) To be published. (Revision of IS0 936: 1978)

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IS0 13730: 1996(E)
0 IS0
5 Reagents
Use only reagents of recognized analytica l grade and distilled or demineralized water or water of at least
equivalent purity.
51 . Nitric acid, 1 + 2 (WV) dilution
Mix 1 volume of nitric acid [65 % (m/m); p20 = 1,40 g/ml] with two volumes of water.
52 . Ammonium monovanadate solution (NHAVCOj), 2,5 g/l.
Dissolve 2,5 g of ammonium monovanadate in 500 ml of boiling water. Cool and transfer quantitatively to a
1000 ml volumetric flask (6.7). Add 20 ml of the nitric acid (5. l), dilute to the mark with water and mix.
53 . Ammonium heptamolybdate solution, [(NH&Mo7024=4H20], 50 g/l.
Dissolve 50 g of ammonium heptamolybdate tetrahydrate in about 800 ml of warm water (at approx. 50 “C). Cool
and transfer quantitatively to a 1000 ml volumetric flask. Dilute to the mark with water and mix.
54 . Colour reagent.
Mix one volume of the nitric acid (5.1) with one volume of the ammonium monovanadate solution (5.2).
Subsequently add one volume of the ammonium heptamolybdate solution (5.3) and mix. The colour reagent should
turn from pale yellow to completely clear.
55 . Phosphate stock solution, c(P) = 218 mg/l; c(PzOs> = 500 mg/l.
Dissolve in water 958,8 mg of potassium dihydrogen phosphate (KHzPO& previously dried for 3 h at
103 OC * 2 OC and allowed to cool in a desiccator.
Transfer quantitatively to a 1000 ml volumetric flask (6.7). Dilute to the mark with water and mix.
56 . Phosphate standard solutions, containing between 0,05 mg and 0,30 mg of P205 per millilitre.
Transfer by pipette or burette to 100 ml volumetric flasks (6.7) 10 ml, 20 ml, 30 ml, 40 ml, 50 ml and 60 ml of the
phosphate stock solution (5.5). Add 10 ml of the nitric acid (5.1). Dilute to the mark with water and mix.
57 .
Blank solution.
Pipette 2 ml of the nitric acid (5.1) and 30 ml of the colour reagent (5.4) into a 100 ml volumetric flask (6.7).
Dilute to the mark with water and mix.
6 Apparatus
IMPORTANT: All glassware shall be thoroughly cleaned using a phosphate-free detergent and then rinsed with
water.
Usual laboratory equipment and, in particular, the following.
61 . Mechanical or electrical equipment capable of homogenizing the laboratory sample. This includes a
high speed rotational cutter, or a mincer fitted with a plate with holes not exceeding 4,5 mm in
diameter (see also clause 8).
62 . Water bath, capable of being maintained at 100 OC.
63 .
Fluted filter paper, of diameter 15 cm, phosphate-free.

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@ IS0 IS0 13730:1996(E)
64 . Spectrometer, capable of being used at a wavelength of 430 nm * 2 nm, or a photo-electric
calorimeter with an interference filter with absorption maximum at 430 nm k 2 nm.
65 . Glass cells, of 10 mm optical path length.
.
66 Analytical balance, capable of weighing to an accur
...