ISO 23922:2020

INTERNATIONAL ISO
STANDARD 23922
First edition
2020-09
Cigarettes — Determination of
selected carbonyls in the mainstream
smoke of cigarettes with an intense
smoking regime — Method using high
performance liquid chromatography
Cigarettes — Dosage de carbonyles sélectionnés dans le courant
principal de la fumée de cigarette avec un régime de fumage intense
— Méthode par chromatographie liquide haute performance
Reference number
©
ISO 2020
© ISO 2020
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ii © ISO 2020 – All rights reserved

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Apparatus . 2
6 Reagents . 3
7 Preparation . 3
7.1 Preparation of glassware. 3
7.2 Preparation of solutions . 4
7.3 Preparation of standards. 4
7.3.1 HPLC calibration standards and working solutions . 4
7.3.2 Carbonyl working standards . 5
8 Sampling . 5
9 Tobacco product preparation . 5
10 Sample generation — Smoking of cigarettes . 5
10.1 General . 5
10.2 Smoking machine setup . 5
10.3 Smoking . 6
10.3.1 General. 6
10.3.2 Linear smoking . 6
10.3.3 Rotary smoking . 6
11 Sample analysis . 7
11.1 Preparation of mainstream smoke extract solution . 7
11.2 Reversed phase high performance liquid chromatography . 7
11.2.1 Chromatographic conditions . 7
11.2.2 Mobile phase reagents . 7
11.2.3 HPLC separation conditions . 8
11.3 Calculation . 8
11.3.1 Calibration curve . 8
11.3.2 Sample quantification . . 8
12 Repeatability and reproducibility . 9
12.1 General . 9
12.2 Results from the 2012 collaborative study .10
13 Test report .13
Annex A (informative) Recrystallization of 2,4-dinitrophenylhydrazine .14
Annex B (normative) Example of calibration standards preparation .15
Annex C (informative) HPLC chromatogram of a typical combined carbonyl calibration
standard .16
Annex D (informative) HPLC chromatogram of carbonyls in DNPH extract of mainstream
cigarette smoke .17
Bibliography .18
Foreword
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iv © ISO 2020 – All rights reserved

Introduction
At the outset of this work, discussions in the CORESTA (www .coresta .org) Special Analytes Sub-Group
(since 2017 the Sub-Group changed its name to Smoke Analytes) determined that most laboratories used
a method involving derivatization of carbonyls with 2,4-dinitrophenylhydrazine (DNPH) because they
considered it the most suitable. This was chosen as the basis of the CORESTA Recommended Method
(CRM). The CRM comprised smoke collection in impinger traps, derivatization of carbonyls with DNPH
followed by their determination using reversed phase High Performance Liquid Chromatography with
Ultra Violet or Diode Array Detection (HPLC-UV or HPLC-DAD).
This document was produced from a 2012 collaborative study involving 19 laboratories from 11 countries
[1][2]
and included 10 samples with different tar yields . This method includes recommendations about
critical steps that should be controlled to provide data as robust and consistent as the repeatability and
reproducibility data provided. Cigarettes were smoked using the intense smoking regime parameters
given in Health Canada Official Method T-115. At the time the collaborative study was conducted, the
study protocol stipulated the use of Health Canada Official Method (T-115) for intense conditions as
there was not an International Standard that defined intense smoking conditions. ISO 20778 was
published in 2018 and is equivalent to Health Canada Intense conditions. Statistical evaluations were
[3] [4]
carried out according to ISO 5725-1 and ISO 5725-2 .
No machine smoking regime can represent all human smoking behaviour.
— It is recommended that cigarettes also be tested under conditions of a different intensity of machine
smoking than those specified in this document.
— Machine smoking testing is useful to characterize cigarette emissions for design and regulatory
purposes, but communication of machine measurements to smokers can result in misunderstandings
about differences in exposure and risk across brands.
— Smoke emission data from machine measurements may be used as inputs for product hazard
assessment, but they are not intended to be nor are they valid as measures of human exposure or
risks. Communicating differences between products in machine measurements as differences in
exposure or risk is a misuse of testing using International Standards.
INTERNATIONAL STANDARD ISO 23922:2020(E)
Cigarettes — Determination of selected carbonyls in
the mainstream smoke of cigarettes with an intense
smoking regime — Method using high performance liquid
chromatography
WARNING — The use of this document can involve hazardous materials, operations and
equipment. This document does not purport to address all the safety problems associated with
its use. It is the responsibility of the user of this document to establish appropriate safety and
health practices and determine the applicability of any other restrictions prior to
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