ISO 21149:2017/Amd 1:2022
(Amendment)Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Amendment 1
Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Amendment 1
Cosmétiques — Microbiologie — Dénombrement et détection des bactéries aérobies mésophiles — Amendement 1
General Information
- Status
- Published
- Publication Date
- 29-Aug-2022
- Technical Committee
- ISO/TC 217 - Cosmetics
- Drafting Committee
- ISO/TC 217/WG 1 - Microbiological standards and limits
- Current Stage
- 6060 - International Standard published
- Start Date
- 30-Aug-2022
- Due Date
- 22-Nov-2022
- Completion Date
- 30-Aug-2022
Relations
- Amends
ISO 21149:2017 - Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Effective Date
- 08-Jan-2022
Overview
ISO 21149:2017/Amd 1:2022 is an important international standard published by the International Organization for Standardization (ISO) that focuses on cosmetics microbiology. Specifically, it presents an amendment to the existing guidelines for the enumeration and detection of aerobic mesophilic bacteria in cosmetic products. This amendment enhances and updates the microbiological testing methods to ensure rigorous safety and quality assessment of cosmetics.
The amendment introduces revisions related to culture media composition and preparation, supporting more accurate and reliable detection of aerobic mesophilic bacteria. This is essential for controlling microbial contamination and ensuring the safety of cosmetic products.
Key Topics
Aerobic Mesophilic Bacteria: Bacteria that require oxygen and grow best at moderate temperatures. Their enumeration is critical for assessing microbiological quality.
Enrichment Broths: Broth media that promote the growth of target bacteria while neutralizing inhibitory substances in cosmetic samples.
Modified Eugon LT Broth: Newly introduced as an alternative enrichment broth with a specified composition to enhance bacterial recovery.
Components include:
- Pancreatic digest of casein
- Papaic digest of soybean meal
- Sodium chloride
- L-cystine
- Sodium sulphite
- Glucose
- Egg lecithin
- Polysorbate 80
- Sodium lauryl ether sulfate
- Water
Modified Eugon LT Agar: A solid agar medium reflecting the modified broth composition, designed for detecting aerobic mesophilic bacteria in cosmetics.
Medium Preparation and Sterilization: Detailed preparation steps including dissolution, mixing, autoclaving at 121 ºC for 15 minutes, and pH adjustment to 7.0 ± 0.2 at room temperature.
These technical updates improve detection sensitivity and neutralize inhibitory components potentially present in cosmetic formulations.
Applications
- Cosmetic Quality Control: Enables manufacturers, laboratories, and regulatory agencies to monitor aerobic mesophilic bacterial contamination effectively.
- Microbiological Safety Testing: Facilitates routine microbiological assays ensuring cosmetics comply with hygiene and safety requirements.
- Product Development: Assists in evaluating new formulations and ingredients for microbiological stability.
- Regulatory Compliance: Supports adherence to international microbiological standards for cosmetics, thereby aiding global market access.
By adopting the updated media and methodological enhancements of ISO 21149:2017 Amendment 1:2022, stakeholders can improve the accuracy of microbial testing results, ensuring safer products that meet consumers’ expectations and regulatory mandates.
Related Standards
- ISO 17516 - Cosmetic Microbiology - Microbiological Limits: Provides guidelines on acceptable microbial levels in cosmetics.
- ISO 22716 - Cosmetics - Good Manufacturing Practices (GMP): Addresses hygiene and manufacturing conditions for cosmetics.
- ISO 21150 - Cosmetics - Microbiology - Detection of presumptive Escherichia coli: Complements detection methods for microbial contaminants.
- ISO 21144 - Cosmetics - Microbiology - Enumeration and detection of yeasts and molds: Expands testing coverage for fungal contaminants.
Together, these standards form a comprehensive framework for cosmetic microbiology testing, ensuring robust safety and quality management throughout the product lifecycle.
Keywords: ISO 21149 Amendment, cosmetic microbiology, aerobic mesophilic bacteria, microbiological enumeration, Eugon LT broth, cosmetic safety testing, microbiological standards for cosmetics, microbiological culture media, cosmetic product quality control.
ISO 21149:2017/Amd 1:2022 - Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria — Amendment 1 Released:30. 08. 2022
ISO 21149:2017/Amd 1:2022 - Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria — Amendment 1 Released:30. 08. 2022
Frequently Asked Questions
ISO 21149:2017/Amd 1:2022 is a standard published by the International Organization for Standardization (ISO). Its full title is "Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Amendment 1". This standard covers: Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Amendment 1
Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria - Amendment 1
ISO 21149:2017/Amd 1:2022 is classified under the following ICS (International Classification for Standards) categories: 07.100.40 - Cosmetics microbiology. The ICS classification helps identify the subject area and facilitates finding related standards.
ISO 21149:2017/Amd 1:2022 has the following relationships with other standards: It is inter standard links to ISO 21149:2017. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
ISO 21149:2017/Amd 1:2022 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
INTERNATIONAL ISO
STANDARD 21149
Second edition
2017-06
AMENDMENT 1
2022-08
Cosmetics — Microbiology —
Enumeration and detection of aerobic
mesophilic bacteria
AMENDMENT 1
Cosmétiques — Microbiologie — Dénombrement et détection des
bactéries aérobies mésophiles
AMENDEMENT 1
Reference number
ISO 21149:2017/Amd.1:2022(E)
ISO 21149:2017/Amd.1:2022(E)
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
ISO 21149:2017/Amd.1:2022(E)
Foreword
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This document was prepared by Technical Committee ISO/TC 217, Cosmetics, in collaboration with
the European Committee for Standar
...
NORME ISO
INTERNATIONALE 21149
Deuxième édition
2017-06
AMENDEMENT 1
2022-08
Cosmétiques — Microbiologie —
Dénombrement et détection des
bactéries aérobies mésophiles
AMENDEMENT 1
Cosmetics — Microbiology — Enumeration and detection of aerobic
mesophilic bacteria
AMENDMENT 1
Numéro de référence
ISO 21149:2017/Amd.1:2022(F)
ISO 21149:2017/Amd.1:2022(F)
DOCUMENT PROTÉGÉ PAR COPYRIGHT
© ISO 2022
Tous droits réservés. Sauf prescription différente ou nécessité dans le contexte de sa mise en œuvre, aucune partie de cette
publication ne peut être reproduite ni utilisée sous quelque forme que ce soit et par aucun procédé, électronique ou mécanique,
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être demandée à l’ISO à l’adresse ci-après ou au comité membre de l’ISO dans le pays du demandeur.
ISO copyright office
Case postale 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Genève
Tél.: +41 22 749 01 11
E-mail: copyright@iso.org
Web: www.iso.org
Publié en Suisse
ii
ISO 21149:2017/Amd.1:2022(F)
Avant-propos
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été rédigé conformément aux règles de rédaction données dans les Directives ISO/IEC, Partie 2 (voir
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Le présent document a été élaboré par le comité technique ISO/TC 217, Cosmétiques, en coll
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