ISO 21326:2019

INTERNATIONAL ISO
STANDARD 21326
First edition
2019-06
Textiles — Test methods for
determining the efficiency of products
against house dust mite
Textiles — Méthodes d'essai pour déterminer l'efficacité des produits
contre les acariens
Reference number
©
ISO 2019
© ISO 2019
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ii © ISO 2019 – All rights reserved

Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
4.1 Repelling method by using Petri-dish . 2
4.2 Repelling method by using glass tube, Method A and Method B . 3
4.3 Proliferation method by using Petri-dish Method A and using vial Method B . 3
4.4 Penetration method . 3
5 Preparation of test . 3
5.1 Reagents. 3
5.2 Apparatus . 4
6 Reference sample . 5
7 Preparation of mite medium. 5
8 Test conditions . 6
8.1 Condition for work area . 6
8.2 Testing conditions . 6
9 Test methods . 6
9.1 Repelling method by using Petri dish. 6
9.2 Repelling method by using glass tube . 6
9.3 Proliferation method by using Petri dish (Method A) and using vial (Method B) . 6
9.4 Penetration method . 6
10 Test report . 7
10.1 Overview . 7
10.2 General . 7
10.3 Repelling methods . 7
10.4 Proliferation methods . 7
10.5 Penetration method . 7
Annex A (normative) Preparation of mite medium . 8
Annex B (normative) Counting methods for live mites .10
Annex C (normative) Repelling method by using Petri dish .13
Annex D (normative) Repelling method by using glass tube .17
Annex E (normative) Proliferation method .21
Annex F (normative) Penetration method .26
Bibliography .30
Foreword
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iv © ISO 2019 – All rights reserved

Introduction
The World Health Organization's (WHO) statement on the correlation of house dust mite to asthma and
other allergic disorders resulted in the increased number of textile products treated against house dust
mite available to the consumer.
However, the testing method to evaluate the efficacy against house dust mite of textiles has not been
standardized to date. This has caused confusion among consumers because of various testing methods
and results.
The purpose of this test method is to standardize the testing method of efficacy of products against
house dust mite in textiles.
INTERNATIONAL STANDARD ISO 21326:2019(E)
Textiles — Test methods for determining the efficiency of
products against house dust mite
1 Scope
This document specifies test methods for efficiency of chemically or physically treated textile products
against house dust mites.
For the products treated by chemicals against house dust mites, the test methods specified in a) to c)
are applied. For the physically treated products, the test method specified in d) is applied.
a) Repelling method by using Petri dish
This method is applied to carpet, bedding surface fabric, bed sheeting, bed covering and blanket.
b) Repelling method by using glass tube (Methods A and B)
This method is applied to wadding (bedding, etc.) with a fibre content of cotton, wool or synthetic
fibre, feathers/down.
c) Proliferation method by using Petri dish (Method A) and using vial (Method B)
Method A is applied to carpet, bedding surface fabric, bed sheeting, bed covering and blanket.
Method B is applied to wadding.
d) Penetration method
This method is applicable to the outer fabric of a futon, bed sheeting and bed covering. However,
this method is not applicable to the multiple component non-woven fabrics and fibre products with
the high stretch properties such as jersey fabrics.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 105-F02, Textiles — Tests for colour fastness — Part F02: Specification for cotton and viscose
adjacent fabrics
ISO 3310-1, Test sieves — Technical requirements and testing — Part 1: Test sieves of metal wire cloth
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 9237, Textiles — Determination of the permeability of fabrics to air
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https: //www .iso .org/obp
— IEC Electropedia: available at http: //www .electropedia .org/
3.1
house dust mite
universal dominant species belonging to Pyroglyphidae, observed in or on floor surfaces, carpets and
bedding with house dust as example
3.2
efficiency of repellency
efficiency of the treatment in repelling house dust mites
3.3
rate of repellency
ratio of the number of live mites in treated material against the number of live mites in untreated
material
Note 1 to entry: The rate of repellency is expressed in percentage (%) and represents the efficacy of repellency of
treated materials.
3.4
rate of suppression of house dust mite reproduction
ratio of the number of live mites in treated material against the number of live mites in untreated
material tested by the proliferation method
Note 1 to entry: The rate of suppression for reproduction of house dust mite is expressed in percentage (%) and
represents the degree of population control of treated materials.
3.5
culture medium
diet for breeding house dust mite
3.6
mite medium
mix of diet and live house dust mite
3.7
live mite
mites which react when stimulated from the outside
Note 1 to entry: It includes all of larva, nymph (protonymph and tritonymph) and adult mites but exclude eggs.
3.8
population density
degree of live mites in a mite medium
Note 1 to entry: The number of live mites in 1 g of th
...