Textiles - Determination of index ingredient from coloured textile - Part 3: Myrobalan

This document specifies a test method for the determination of the index ingredient of chemicals in coloured fabric with myrobalan.

Textiles — Détermination d'indicateurs d'ingrédients de textiles colorés — Partie 3: Mirobolant

General Information

Status
Published
Publication Date
29-Nov-2023
Technical Committee
Drafting Committee
Current Stage
6060 - International Standard published
Start Date
30-Nov-2023
Due Date
18-Jan-2025
Completion Date
30-Nov-2023
Ref Project

Overview

ISO 22195-3:2023 - Textiles - Determination of index ingredient from coloured textile - Part 3: Myrobalan - specifies an HPLC‑based test method to detect and quantify the index ingredient chebulagic acid in textiles dyed with myrobalan (fruits of Terminalia species). The standard defines sample preparation, reagent and apparatus requirements, chromatographic analysis (HPLC‑MS/HPLC‑DAD) and calculation/reporting procedures for expressing results in mg/kg.

Key topics and technical requirements

  • Scope: Identification and quantification of the index ingredient (chebulagic acid) in coloured fabrics dyed with myrobalan.
  • Reagents: HPLC‑grade solvents - methanol, acetonitrile, formic acid (30 % stock), and laboratory water compliant with ISO 3696; certified chebulagic acid reference standard (purity indicated).
  • Apparatus: Analytical balance (0.001 g resolution), ultrasonic bath (30 ± 2 °C), rotary evaporator, 0.2 μm membrane filter, and HPLC‑MS (or HPLC‑DAD for wavelength checks).
  • Sample preparation: Cut ~1 g of fabric into ~5 mm squares, extract with 10 mL methanol using ultrasonic extraction for (20 ± 1) min at (30 ± 2) °C, filter the extract into HPLC vials; dilution and concentration adjustments permitted and must be documented.
  • Analysis: Detect and identify chebulagic acid using HPLC‑MS. Annex A provides example chromatographic conditions (mobile phases: water/acetonitrile with 0.1 % formic acid; example columns and MS settings).
  • Calibration & quantification: Prepare calibration standards (typical range 1–100 mg/L with at least five points). Required calibration correlation coefficient: greater than 0.995 (note: laboratories may adapt ranges to equipment).
  • Calculation & reporting: Convert measured solution concentration to mg/kg of fabric using the specified formula and report test details, chromatographic conditions, any deviations, and test date. Reference ISO 22195-3:2023 must appear in the report.

Applications and who uses it

ISO 22195-3:2023 is intended for:

  • Textile testing laboratories performing natural dye identification and quantification.
  • Quality control teams in textile manufacturing verifying use of myrobalan or monitoring dye consistency.
  • Conservation scientists and museums authenticating historical textiles dyed with plant-based colourants.
  • Regulatory and customs authorities assessing claims of natural dye use, origin labeling, or compliance.
  • Academic and applied researchers studying natural colourants, dye chemistry, and extraction methods.

Practical uses include authentication of natural-dyed garments, forensic dye analysis, supplier verification, and research into dye composition and fastness.

Related standards

  • ISO 3696 - Water for analytical laboratory use (referenced for water quality).
  • Other parts of the ISO 22195 series (see ISO website) cover determination of index ingredients from other natural colourants.

Keywords: ISO 22195-3:2023, myrobalan, chebulagic acid, textiles, natural colourant, HPLC‑MS, dye identification, calibration curve, extraction, analytical method.

Standard
ISO 22195-3:2023 - Textiles — Determination of index ingredient from coloured textile — Part 3: Myrobalan Released:30. 11. 2023
English language
7 pages
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Frequently Asked Questions

ISO 22195-3:2023 is a standard published by the International Organization for Standardization (ISO). Its full title is "Textiles - Determination of index ingredient from coloured textile - Part 3: Myrobalan". This standard covers: This document specifies a test method for the determination of the index ingredient of chemicals in coloured fabric with myrobalan.

This document specifies a test method for the determination of the index ingredient of chemicals in coloured fabric with myrobalan.

ISO 22195-3:2023 is classified under the following ICS (International Classification for Standards) categories: 59.080.01 - Textiles in general. The ICS classification helps identify the subject area and facilitates finding related standards.

You can purchase ISO 22195-3:2023 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of ISO standards.

Standards Content (Sample)


INTERNATIONAL ISO
STANDARD 22195-3
First edition
2023-11
Textiles — Determination of index
ingredient from coloured textile —
Part 3:
Myrobalan
Textiles — Détermination d'indicateurs d'ingrédients de textiles
colorés —
Partie 3: Mirobolant
Reference number
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagent . 2
6 Apparatus . 2
7 Procedure .2
7.1 Standard preparation . 2
7.2 Preparation of test specimen . 2
7.3 Analysis . 3
7.4 Determination and calculation . 3
7.4.1 Determination of chebulagic acid . 3
7.4.2 Calibration curve. 3
7.4.3 Calculation of chebulagic acid . 3
8 Test report . 3
Annex A (informative) Example of test result . 5
iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
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ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO document should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use
of (a) patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed
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www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 38, Textiles.
A list of all parts in the ISO 22195 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
There is no doubt that dyeing plays the most important role in expressing the colour of clothes. Until
the invention of synthetic dyes capable of expressing diverse colours, materials obtained from nature
to dye fabric have been used. Typically, colourants were obtained from plants or various materials were
extracted from minerals or insects. When dyeing fabrics using materials derived from these natural
substances, it becomes necessary to identify which substances the colourant was derived from. In other
words, there has been a demand to confirm whether a fabric is dyed using a natural substance.
A test method is developed to identify which type of natural substances has been used.
v
INTERNATIONAL STANDARD ISO 22195-3:2023(E)
Textiles — Determination of index ingredient from
coloured textile —
Part 3:
Myrobalan
1 Scope
This document specifies a test method for the determination of the index ingredient of chemicals in
coloured fabric with myrobalan.
2 Normative references
The following document is referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analy
...

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