EN 13585:2001
(Main)Foodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up
Foodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up
This European Standard specifies a method for the determination of fumonisin B1 (FB1) and fumonisin B2 (FB2) in maize using high performance liquid chromatography (HPLC).
The method has been successfully validated in an interlaboratory study according to AOAC Guidelines for Collaborative Studies [1] on maize containing 405 µg/kg to 6732 mg/kg fumonisin B1 and 152 mg/kg to 2619 mg/kg fumonisin B2. The method works well with maize or minimally processed maize (e.g. fresh, dried and milled maize), but does not provide reliable results with most maize-based processed products.
Lebensmittel - Bestimmung von Fumonisin B1 und B2 in Mais - HPLC-Verfahren mit Reinigung durch Festphasenextraktion
Dieser Europäische Norm-Entwurf legt ein hochleistungs-flüssigchromatographisches (HPLC)-Verfahren für die Bestimmung von Fumonisin B1 (FB1) und Fumonisin B2 (FB2) in Mais fest. Das Verfahren wurde erfolgreich in einem Ringversuch nach ISO 5725: 1986 an Mais, der 405 Mikron g/kg bis 6732 Mikron g/kg Fumonisin B1 und 152 Mikron g/kg bis 2619 Mikron g/kg Fumonisin B2 enthält, validiert.
Produits alimentaires - Dosage des fumonisines B1 et B2 dans le maïs - Méthode CLHP avec purification par extraction en phase solide
La présente Norme européenne spécifie une méthode de dosage de la fumonisine B1 (FB1) et de la fumonisine B2 (FB2) dans le maïs, par chromatographie en phase liquide haute performance(CLHP).
Cette méthode a été validée par un essai interlaboratoires selon les Lignes Directrices AOAC pour Essais Interlaboratoires [1] sur du maïs contenant entre 405 µg/kg et 6 732 µg/kg de fumonisine B1 et entre 152 µg/kg et 2 619 µg/kg de fumonisine B2. Cette méthode fonctionne bien avec du maïs ou du maïs peu transformé (par exemple, du maïs moulu, séché ou frais) mais ne donne pas de résultats fiables avec la plupart des produits transformés à base de maïs.
Živila - Določevanje fumonizinov B1 in B2 v koruzi - HPLC metoda z ekstrakcijo na trdno fazo
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Bestimmung von Fumonisin B1 und B2 in Mais - HPLC-Verfahren mit Reinigung durch FestphasenextraktionProduits alimentaires - Dosage des fumonisines B1 et B2 dans le mais - Méthode CLHP avec purification par extraction en phase solideFoodstuffs - Determination of fumonisins B1 and B2 in maize - HPLC method with solid phase extraction clean-up67.060QMLKCereals, pulses and derived productsICS:Ta slovenski standard je istoveten z:EN 13585:2001SIST EN 13585:2002en01-junij-2002SIST EN 13585:2002SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 13585December 2001ICS 67.060English versionFoodstuffs - Determination of fumonisins B1 and B2 in maize -HPLC method with solid phase extraction clean-upProduits alimentaires - Dosage des fumonisines B1 et B2dans le maïs - Méthode CLHP avec purification parextraction en phase solideLebensmittel - Bestimmung von Fumonisin B1 und B2 inMais - HPLC-Verfahren mit Reinigung durchFestphasenextraktionThis European Standard was approved by CEN on 2 November 2001.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2001 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 13585:2001 ESIST EN 13585:2002
Typical chromatogram.10Annex B (informative)
Recovery and relative standard deviation.11Annex C (informative)
Precision data.12Bibliography.15ForewordThis European Standard has been prepared by Technical Committee CEN /TC 275, "Food analysis - Horizontalmethods", the secretariat of which is held by DIN.This European Standard shall be given the status of a national standard, either by publication of an identical text orby endorsement, at the latest by June 2002, and conflicting national standards shall be withdrawn at the latest byJune 2002.The annexes A, B and C are informative.According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland,France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden,Switzerland and the United Kingdom.SIST EN 13585:2002
= 335 nm and emissionwavelength of
= 440 nm.5.1.4
Data system5.2 Blender, homogenizer, or mixer.5.3 Fluted filter paper5.4 Strong-anion-exchanging solid phase extraction column, e.g. Bond-Elut
1) SAX-cartridges,containing 500 mg of sorbent, or similar has been found to be suitable.5.5 SPE extraction manifold5.6 Solvent evaporator, with heating module, or similar.5.7 Membrane filter, for aqueous solutions, with a pore size of 0,45 m.6 SamplingIt is important that the laboratory receives a sample which is truly representative and has not been damaged orchanged during transport or storage.7 Preparation of the test sampleGrind the sample to pass through a 1,0 mm sieve and homogenize the sample.8 Procedure8.1 ExtractionPlace 50 g of the test sample into a suitable glass or plastic container (e.g. a 250 ml plastic centrifuge bottle).Extract for 3 min with 100 ml of methanol solution (4.3) using the blender (5.2) at approximately 10 000 min-1. Thetime needed for complete extraction can vary with the type of equipment used.Centrifuge the blended extract for 10 min at 500 g and filter the supernatant through a fluted filter paper (5.3).Check the pH of the eluate and adjust, if necessary, with sodium hydroxide solution (4.11) to between pH 5,8 andpH 6,5.
1 Bond-Elut is an example of a suitable product available commercially. This information is given for the convenience of usersof this European Standard and does not constitute an endorsement by CEN of this product.SIST EN 13585:2002
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