EN ISO 18743:2015/A1:2023
(Amendment)Microbiology of the food chain - Detection of Trichinella larvae in meat by artificial digestion method - Amendment 1: Method validation studies and performance characteristics (ISO 18743:2015/Amd 1:2023)
Microbiology of the food chain - Detection of Trichinella larvae in meat by artificial digestion method - Amendment 1: Method validation studies and performance characteristics (ISO 18743:2015/Amd 1:2023)
Mikrobiologie der Lebensmittelkette - Nachweis von Trichinella-Larven in Fleisch mit künstlichem Verdauungsverfahren - Änderung 1: Verfahrensvalidierung und Leistungsmerkmale (ISO 18743:2015/Amd 1:2023)
No scope available
Microbiologie de la chaîne alimentaire - Recherche des larves de Trichinella dans la viande par une méthode de digestion artificielle - Amendement 1: Études de validation de la méthode et caractéristiques de performance (ISO 18743:2015/Amd 1:2023)
Mikrobiologija v prehranski verigi - Ugotavljanje prisotnosti ličink Trichinella v mesu z metodo umetne prebave - Dopolnilo A1: Študija validacije metode in značilnosti delovanja (ISO 18743:2015/Amd 1:2023)
General Information
Relations
Overview
EN ISO 18743:2015/A1:2023 is an important European Standard amendment focused on the microbiological detection of Trichinella larvae in meat through the artificial digestion method. Published by CEN in 2023, this amendment updates ISO 18743:2015 to include validated method studies and detailed performance characteristics. It ensures a standardized and reliable approach for the food industry and laboratories detecting Trichinella, a parasitic threat in the food chain, particularly in pork products.
This amendment sharpens the guideline by incorporating interlaboratory validation data and refining the sampling and testing procedures to enhance accuracy, reproducibility, and sensitivity in detecting Trichinella larvae in meat.
Key Topics
Artificial Digestion Method: Outlines the process of artificially digesting meat samples to release and detect Trichinella larvae using pepsin and hydrochloric acid solutions.
Method Validation: Includes interlaboratory studies that validate the detection technique on raw minced pig meat, confirming the method’s reliability and sensitivity.
Performance Characteristics:
- Level of Detection (LOD): Defines the sensitivity threshold, with validated detection capability at contamination levels as low as three larvae per 100 g of meat.
- Sensitivity and Specificity: Demonstrated 100% specificity and high sensitivity for detecting Trichinella larvae in contaminated samples through proficiency testing.
Updated Sampling Recommendations: Emphasizes the importance of sampling predilection sites (e.g., diaphragm pillars or masseter muscles) and advises increased sample sizes when specific muscles are unavailable.
Equipment and Decontamination: Specifies the use of glass, plastic, or steel equipment suitable for digestion and mandates strict cleaning or decontamination protocols to prevent cross-contamination.
Microscopic Examination: Details procedures for sediment analysis using stereomicroscopes or trichinoscopes at 10X to 20X magnification, ensuring thorough and systematic examination of samples.
Applications
Food Safety Testing: Applies to laboratories performing microbiological analysis in pork and other susceptible meat products to detect Trichinella larvae before products enter the consumer market.
Quality Control in Meat Processing: Enables meat producers and processors to implement standardized testing that complies with EU regulations, enhancing consumer safety and regulatory compliance.
Surveillance Programs: Supports governmental and veterinary diagnostic services monitoring Trichinella prevalence in animal populations to mitigate foodborne parasitic infections.
Research and Method Development: Provides a benchmark method with validated performance data for researchers refining detection technologies or developing alternative diagnostic protocols.
Related Standards
ISO 16140-1:2016: Addresses the validation of microbiological methods, referenced here for defining detection levels.
WOAH Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (11th Edition, 2022): Offers complementary guidance on Trichinellosis diagnosis applicable to veterinary and food safety contexts.
EU Regulation 2020/1478: Incorporates the ISO 18743 method for detection of Trichinella in raw meat in EU food safety legislation.
This amendment to EN ISO 18743 underscores the critical role of harmonized, validated testing methods in safeguarding the food chain microbiology. By enhancing the accuracy of detecting Trichinella in meat through artificial digestion, it supports public health objectives and the reliability of food safety programs across Europe and internationally.
Frequently Asked Questions
EN ISO 18743:2015/A1:2023 is a amendment published by the European Committee for Standardization (CEN). Its full title is "Microbiology of the food chain - Detection of Trichinella larvae in meat by artificial digestion method - Amendment 1: Method validation studies and performance characteristics (ISO 18743:2015/Amd 1:2023)". This standard covers: No scope available
No scope available
EN ISO 18743:2015/A1:2023 is classified under the following ICS (International Classification for Standards) categories: 07.100.30 - Food microbiology. The ICS classification helps identify the subject area and facilitates finding related standards.
EN ISO 18743:2015/A1:2023 has the following relationships with other standards: It is inter standard links to EN ISO 18743:2015. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
You can purchase EN ISO 18743:2015/A1:2023 directly from iTeh Standards. The document is available in PDF format and is delivered instantly after payment. Add the standard to your cart and complete the secure checkout process. iTeh Standards is an authorized distributor of CEN standards.
Standards Content (Sample)
SLOVENSKI STANDARD
01-december-2023
Mikrobiologija v prehranski verigi - Ugotavljanje prisotnosti ličink Trichinella v
mesu z metodo umetne prebave - Dopolnilo A1: Študija validacije metode in
značilnosti delovanja (ISO 18743:2015/Amd 1:2023)
Microbiology of the food chain - Detection of Trichinella larvae in meat by artificial
digestion method - Amendment 1: Method validation studies and performance
characteristics (ISO 18743:2015/Amd 1:2023)
Mikrobiologie der Lebensmittelkette - Nachweis von Trichinella-Larven in Fleisch mit
künstlichem Verdauungsverfahren - Änderung 1: Verfahrensvalidierung und
Leistungsmerkmale (ISO 18743:2015/Amd 1:2023)
Microbiologie de la chaîne alimentaire - Recherche des larves de Trichinella dans la
viande par une méthode de digestion artificielle - Amendement 1: Études de validation
de la méthode et caractéristiques de performance (ISO 18743:2015/Amd 1:2023)
Ta slovenski standard je istoveten z: EN ISO 18743:2015/A1:2023
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EN ISO 18743:2015/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
August 2023
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Detection of Trichinella
larvae in meat by artificial digestion method - Amendment
1: Method validation studies and performance
characteristics (ISO 18743:2015/Amd 1:2023)
Microbiologie de la chaîne alimentaire - Recherche des Mikrobiologie der Lebensmittelkette - Nachweis von
larves de Trichinella dans la viande par une méthode Trichinella-Larven in Fleisch mit künstlichem
de digestion artificielle - Amendement 1: Études de Verdauungsverfahren - Änderung 1: Überarbeitung des
validation de la méthode et caractéristiques de Textes und kleinerer technischer Fragestellungen,
performance (ISO 18743:2015/Amd 1:2023) Aktualisierung der Verweise und Aufnahme von
Leistungsmerkmalen des Verfahrens durch einen
Ringversuch (ISO 18743:2015/Amd 1:2023)
This amendment A1 modifies the European Standard EN ISO 18743:2015; it was approved by CEN on 22 July 2023.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2023 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 18743:2015/A1:2023 E
worldwide for CEN national Members.
Contents Page
European foreword . 3
European foreword
This document (EN ISO 18743:2015/A1:2023) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food
chain” the secretariat of which is held by AFNOR.
This Amendment to the European Standard EN ISO 18743:2015 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by February 2024,
and conflicting national standards shall be withdrawn at the latest by February 2024.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 18743:2015/Amd 1:2023 has been approved by CEN as EN ISO 18743:2015/A1:2023
without any modification.
INTERNATIONAL ISO
STANDARD 18743
First edition
2015-09-15
AMENDMENT 1
2023-08
Microbiology of the food chain —
Detection of Trichinella larvae in meat
by artificial digestion method
AMENDMENT 1: Method validation
studies and performance characteristics
Microbiologie de la chaîne alimentaire — Recherche des larves de
Trichinella dans la viande par une méthode de digestion artificielle
AMENDEMENT 1: Études de validation de la méthode et
caractéristiques de performance
Reference number
ISO 18743:2015/Amd.1:2023(E)
ISO 18743:2015/Amd.1:2023(E)
© ISO 2023
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
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ISO 18743:2015/Amd.1:2023(E)
Foreword
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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC
9, Microbiology, in collaboration with the European Committee for Standardization (CEN) Technical
Committee CEN/TC 463, Microbiology of the food chain, in accordance with the Agreement on technical
cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
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