EN 14481:2003

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Werkstoffe und Gegenstände in Kontakt mit Lebensmitteln - Kunstoffe - Prüfverfahren zur Bestimmung des fettigen KontaktesMatériaux et articles en contact avec les denrées alimentaires - Plastiques - Méthodes d'essai pour la détermination d'un contact grasMaterials and articles in contact with foodstuffs - Plastics - Test methods for the determination of fatty contact83.080.01Polimerni materiali na splošnoPlastics in general67.250Materiali in predmeti v stiku z živiliMaterials and articles in contact with foodstuffsICS:Ta slovenski standard je istoveten z:EN 14481:2003SIST EN 14481:2003en01-september-2003SIST EN 14481:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14481May 2003ICS 67.250; 83.080.01English versionMaterials and articles in contact with foodstuffs - Plastics - Testmethods for the determination of fatty contactMatériaux et articles en contact avec les denréesalimentaires - Plastiques - Méthodes d'essai pour ladétermination d'un contact grasWerkstoffe und Gegenstände in Kontakt mit Lebensmitteln- Kunstoffe - Prüfverfahren zur Bestimmung des fettigenKontaktesThis European Standard was approved by CEN on 9 January 2003.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and UnitedKingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2003 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14481:2003 ESIST EN 14481:2003

Typical HPLC chromatogram for standard 1,4-diphenyl-1,3-butadiene and 1,6-diphenyl-1,3,5-hexatriene.11Annex ZA (informative)
Relationship of this European Standard with Council Directive 85/572/EEC.12Bibliography.13SIST EN 14481:2003

Themethod described here can be used to determine whether a food makes fatty contact with plastics.NOTE 1The food types are specified in Council Directive 85/572/EEC [1].NOTE 2Guidance on the selection of conditions and test methods for overall migration, and the test methods for the specificmigration of substances from plastics into food and food simulants and the determination of substances in plastics and theselection of conditions of exposure to food simulants is given in EN 1186-1 and EN 13130-1 respectivelySIST EN 14481:2003

Testing some foods can require modifications to the method.
The method is applicable tocontact situations from -20 °C to 100 °C.2 PrincipleFatty contact between a food and a plastic material or article is determined by placing food in contact with apolyethylene film containing a fat-soluble fluorescent dye.
After exposure, the dye is extracted from the food anddetermined by high performance liquid chromatography (HPLC) with fluorescence detection.
The method employs1,6-diphenyl-1,3,5-hexatriene as internal standard.
The degree of transfer is related to the extent of fatty contactmade, and is used to determine whether simulant D be employed or not in subsequent tests of plastics intended tocome into contact with that food.The food used in the test should be the same as, or similar in composition to, the food which will come into contactwith the plastic in actual use.
To allow for possible difficulties encountered in obtaining reproducible film to foodcontact, the test is carried out on five replicate samples of the food.3 ReagentsAll reagents shall be of recognised analytical quality, unless otherwise specified.3.1Low density polyethylene film containing 1,4-diphenyl-1,3-butadiene, fluorescent dye, at 17,3 g/dm2 0,9g/dm2, in the form of a lay-flat tube of width 20 cm, e.g. the certified reference material BCR.593, Plastic film E,certified on the basis of the mass fraction of DPBD (12,58 mg/kg ± 0,63 mg/kg) and on the film thickness (149,7 µm± 0,7 µm).3.21,4-diphenyl-1,3-butadiene (DPBD)3.31,6-diphenyl-1,3,5-hexatriene (DPHT)NOTEAs DPBD and DPHT are slightly light sensitive, the film and standards should be stored with the exclusion of light.3.4n-Hexane, C6H14, HPLC gradeNOTEAs n-hexane is considered to be harmful by inhalation, ingestion and through skin contact, correct safety proceduresshould be followed at all times when using this solvent.3.5Dichloromethane, CH2Cl2, HPLC gradeNOTEAs dichloromethane is considered to be harmful by inhalation, ingestion and through skin contact and as a potentialcarcinogen, correct safety procedures should be followed at all times when using this solvent.4 Apparatus4.1Heat sealer suitable for sealing the polyethylene film (3.1)4.2Dispersion homogenizer4.3Centrifuge, bench-top4.4Centrifuge tubes, 10 ml to 20 ml capacity4.5Filter paper, 15,0 cm diameter circles, Whatman Grade 2 or equivalent4.6Volumetric flasks, 25 ml, 50 ml and 100 ml capacitySIST EN 14481:2003

Add, by syringe, 100 l of the stock solution(5.1.1).
Make to the mark with n-hexane.5.1.3 Stock solution of DPHT at 1mg/mlWeigh 0,050 g
0,001 g of DPHT (3.3) into a 50 ml volumetric flask and make to the mark with dichloromethane(3.5).5.1.4 Calibration solution of DPHT in n-hexane at 100 g/mlPlace approximately 30 ml of n-hexane into a 50 ml volumetric flask.
Add, by pipette (4.16), 5 ml of the stocksolution (5.1.3).
Make to the mark with n-hexane.NOTEAs DPBD and DPHT are sensitive to light, standard solutions should be stored with the exclusion of light.SIST EN 14481:2003

Unpack the film.
Unroll the outer turn from the reel, cut offand discard.
Cut a 220 mm length of the film.
Use the heat sealer (4.1) to close one of the cut edges.
Place thefood sample in the pouch so formed and then seal the opening to give a 200 mm x 200 mm pouch.Prepare a further four replicate pouches containing food.NOTEThe quantity of food used and the plastic-to-food contact, should be representative of that in actual use.
Somefoods can need modification to give representative contact e.g. for non-homogeneous foods with differing surfaces, it could benecessary to cut the food such that only the food surface which will contact the plastic in actual use comes into contact with thepolyethylene film.
Alternatively, the non-representative surface(s) can be shielded from contact by introducing an interlayer of n-hexane-washed aluminium foil between the surface(s) and the film.Where a food is non-homogeneous and an actual use-size portion will not fit in the pouch, it is suggested that the food samplebe modified by cutting or otherwise reducing in size, ensuring that the portion taken for exposure is representative of the food asa whole.
For example, for a cut of fish, the ratio of skin to lean flesh in the cut portion should be the same as that for the wholeportion.
Caution should be taken to ensure that cut edges do not modify the nature of the food.
If necessary, they may beshielded from contact by the use of aluminium foil.For homogeneous foods such as powders and liquids, a 100 mm x 100 mm pouch can be used and the quantity of foodreduced accordingly.5.2.2After sealing the food in the bag, insert the vacuum needle (4.12) at one corner and use vacuum suction toremove the trapped air.
When the majority of the air is removed and the food sample makes good contact with th
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