prEN ISO 27065

SLOVENSKI STANDARD
01-julij-2025
Varovalna obleka - Zahtevane lastnosti za varovalno oblačilo, ki ga nosijo osebe,
ki rokujejo s pesticidi in delavci pri ponovnem vstopu na kontaminirano območje
(ISO/DIS 27065:2025)
Protective clothing - Performance requirements for protective clothing worn by operators
applying pesticides and for re-entry workers (ISO/DIS 27065:2025)
Schutzkleidung - Leistungsanforderungen an Schutzkleidung für die Anwender von
Pflanzenschutzmitteln sowie Personen für Nachfolgearbeiten (ISO/DIS 27065:2025)
Habillement de protection - Exigences de performance pour les vêtements de protection
portés par les opérateurs appliquant des pesticides et pour les travailleurs de rentrée
(ISO/DIS 27065:2025)
Ta slovenski standard je istoveten z: prEN ISO 27065
ICS:
13.340.10 Varovalna obleka Protective clothing
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
International
Standard
ISO/DIS 27065
ISO/TC 94/SC 13
Protective clothing — Performance
Secretariat: SNV
requirements for protective
Voting begins on:
garments worn by operators
2025-05-16
applying pesticides and for re-entry
Voting terminates on:
workers
2025-08-08
Habillement de protection — Exigences de performance pour les
vêtements de protection portés par les opérateurs appliquant des
pesticides et pour les travailleurs de rentrée
ICS: 13.340.10
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
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TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
NATIONAL REGULATIONS.
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
PROVIDE SUPPORTING DOCUMENTATION.
Reference number
ISO/DIS 27065:2025(en)
DRAFT
ISO/DIS 27065:2025(en)
International
Standard
ISO/DIS 27065
ISO/TC 94/SC 13
Protective clothing — Performance
Secretariat: SNV
requirements for protective
Voting begins on:
garments worn by operators applying
pesticides and for re-entry workers
Voting terminates on:
Habillement de protection — Exigences de performance pour les
vêtements de protection portés par les opérateurs appliquant des
pesticides et pour les travailleurs de rentrée
ICS: 13.340.10
THIS DOCUMENT IS A DRAFT CIRCULATED
FOR COMMENTS AND APPROVAL. IT
IS THEREFORE SUBJECT TO CHANGE
AND MAY NOT BE REFERRED TO AS AN
INTERNATIONAL STANDARD UNTIL
PUBLISHED AS SUCH.
This document is circulated as received from the committee secretariat.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL,
© ISO 2025
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
STANDARDS MAY ON OCCASION HAVE TO
ISO/CEN PARALLEL PROCESSING
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BE CONSIDERED IN THE LIGHT OF THEIR
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TO SUBMIT, WITH THEIR COMMENTS,
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Website: www.iso.org
Published in Switzerland Reference number
ISO/DIS 27065:2025(en)
ii
ISO/DIS 27065:2025(en)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 2
4 Classification and testing requirements . 4
5 Pre-treatment and conditioning . 5
5.1 Pre-treatment by cleaning .5
5.2 Conditioning.5
5.3 Deterioration of repellency by cleaning .6
6 Performance requirements of protective garment materials . 6
6.1 General .6
6.2 Test chemical EC-DY preparation . .6
6.3 Material resistance to penetration (pipette test) .6
6.4 Material Repellency (pipette test) .7
6.5 Material resistance to permeation .7
6.6 Material tensile strength .8
6.7 Material tear resistance .8
6.8 Material puncture resistance .8
7 Performance requirements of seams . 9
7.1 General .9
7.2 Seam resistance to penetration.9
7.3 Seam resistance to permeation .9
7.4 Seam tensile strength.10
8 Performance requirements of protective garment . 10
8.1 General .10
8.2 Practical Performance tests .10
8.3 Liquid penetration resistance .10
8.3.1 Low-level spray test .10
8.3.2 High-level spray test . .11
8.4 General .11
8.5 Specific to pesticide protection .11
8.6 Marking for partial body garments .11
8.7 Additional marking requirements . 12
9 Information supplied by the manufacturer .13
Annex A (normative) Test subject exercises for practical performance evaluation .15
Annex B (normative) Testing requirements for Levels C1, C2 and C3 protective clothing .16
Annex C (informative) Selection of test chemical for penetration tests . 17
Annex D (informative) Material water-vapour resistance (optional) .18
Annex E (informative) Food and Agricultural Organization (FAO) Pesticide Labelling
Pictograms . 19
Annex F (informative) Risk assessment — PPE to mitigate risk .20
Annex G (normative) Surrogate test chemical .22
Annex ZA (informative) Relationship between this European Standard and the essential
requirements of Regulation (EU) 2016/425 aimed to be covered .23
Bibliography .27

iii
ISO/DIS 27065:2025(en)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO [had/had not] received notice of
(a) patent(s) which may be required to implement this document. However, implementers are cautioned that
this may not represent the latest information, which may be obtained from the patent database available at
www.iso.org/patents. ISO shall not be held responsible for identifying any or all such patent rights.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee [or Project Committee] ISO/TC 94, Personal safety —
Protective clothing and equipment, Subcommittee SC 13, Protective clothing.
This third edition cancels and replaces the second edition (ISO 27065:2017), which has been technically
revised.
The main changes are as follows:
— 1 Introduction has been revised;
— 2.0 Normative references have been updated;
— 3 Terms and definitions have been updated;
— 5.2 Conditioning has been revised;
— 5, Deterioration of repellency by cleaning has been added;
— 6.5 Material resistance to permeation requirements has been revised;
— 6.6 Material tearing strength requirement and standard for testing woven fabrics have been changed;
— 7.3 Seam resistance to permeation requirement has been revised;
— 9.2 Text for placement of the pictogram has been revised;
— 9.3 Marking for partial body garments have been added;
— Annex F and G have been revised.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
ISO/DIS 27065:2025(en)
Introduction
This document addresses the performance requirements for protective clothing worn by operators handling
liquid pesticide products as well as protective clothing worn by re-entry workers. It includes requirements
for protective garments (e.g. shirts, jackets, trousers, and coveralls) and partial body protective garments
(e.g. aprons, smocks, protective sleeves, hoods/caps, and material placed below knapsack/backpack
sprayers). Requirements for protective clothing, including partial body, constructed with multiple layers or
materials are also included in this document.
This document classifies protective clothing, including partial body, into three performance levels. A brief
description for the three levels is given below.
Level C1 protective clothing, including partial body, is suitable when the potential risk is relatively low. Level
C1 protective clothing can be used as the base protective clothing with additional protective garments worn
based on risk assessment See Annex F for additional information on risk assessment and use of PPE for risk
mitigation.
Level C2 protective clothing, including partial body, is suitable when it has been determined that the
protection required is higher than that provided by Level C1 protective clothing. Level C2 protective clothing
typically provides a balance between comfort and protection. It can be used as the base protective clothing
in place of C1. with additional C3 partial body protective garments based on risk assessment.
Level C3 protective clothing, including partial body, is suitable for use when it has been determined that
additional protection is required based on risk assessment (including requirements based on GHS hazard
categories.) Precautionary measures such as short duration for use are necessary for Level C3 suits/coveralls
that may cause heat build-up resulting in heat exhaustion/stress. Level C3 protective clothing, including
partial body, is suitable for use with diluted as well as concentrated pesticides.
Personal Protective Equipment (PPE) is often used for risk mitigation. See Annex F for information on risk
assessment and use of PPE for risk mitigation. Since protective clothing can be contaminated in various ways
(e.g. fine spray, contact with wet surface, contact with pesticide product sprayed under pressure, contact
between the protective clothing and a contaminated surface), laboratory test methods used in the standard
rate materials and garments rather than simulate the various field conditions.
ISO 16602 focuses on industrial chemicals, whereas this document focuses on protection against pesticides
that are frequently applied in aqueous solutions. Penetration, permeation, and repellency tests in ISO 16602
are typically done with neat chemicals not used in pesticide application. In this document, penetration,
permeation, and repellency tests are conducted with a mixture. The test chemical selected for testing is a
pesticide surrogate that is representative of a best-known worst-case scenario for chemical ingress. Testing
for penetration is conducted with diluted formulation. For permeation, the standard provides a provision
for testing with diluted formulation. In additional, it allows for testing with additional pesticide products, if
required, based on risk assessment conducted for the required pesticide product.
This document is intended for fabric and protective clothing manufacturers and pesticide product
manufacturers, as well as trainers, regulators, and other individuals or organizations that make decisions
regarding protective clothing for protection against pesticide products.

v
DRAFT International Standard ISO/DIS 27065:2025(en)
Protective clothing — Performance requirements for
protective garments worn by operators applying pesticides
and for re-entry workers
1 Scope
This document establishes minimum performance, classification, and marking requirements for protective
clothing worn by operators handling pesticide products as well as re-entry workers. For the purpose of this
document, the term pesticide applies to insecticides, herbicides, fungicides, and other substances applied in
liquid form that are intended to prevent, destroy, repel, or reduce any pest or weeds in agricultural settings,
green spaces, roadsides, etc. It does not include biocidal products used for agricultural and non-agricultural
settings.
Pesticide handling includes mixing and loading, application, and other activities such as cleaning
contaminated equipment and containers. Concentrated pesticides are typically handled during mixing
and loading. Protective garments covered by this document include, but are not limited to, shirts, jackets,
trousers, coveralls, aprons, protective sleeves, caps/hats and other headwear (excluding hard hats made of
rigid materials, e.g. hats worn by construction workers), and accessories used under knapsack/backpack
sprayers.
This document does not address items used for the protection of the respiratory tract, hands, and feet. This
document does not address protection against fumigants.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
ISO 9073-4, Nonwovens — Test methods — Part 4: Determination of tear resistance by the trapezoid procedure
ISO 13688:2013, Protective clothing — General requirements
ISO 13934-1, Textiles — Tensile properties of fabrics — Part 1: Determination of maximum force and elongation
at maximum force using the strip method
ISO 13935-2, Textiles — Seam tensile properties of fabrics and made-up textile articles — Part 2: Determination
of maximum force to seam rupture using the grab method
ISO 13996, Protective clothing — Mechanical properties — Determination of resistance to puncture
ISO 17491-4:2024, Protective clothing — Test methods for clothing providing protection against chemicals —
Part 4: Determination of resistance to penetration by a spray of liquid (spray test)
ISO 19918, Protective clothing — Protection against chemicals — Measurement of cumulative permeation of
chemicals with low vapour pressure through materials
ISO 22608:2021, Protective clothing — Protection against liquid chemicals — Measurement of repellency,
retention, and penetration of liquid pesticide formulations through protective clothing materials

ISO/DIS 27065:2025(en)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— IEC Electropedia: available at https:// www .electropedia .org/
— ISO Online browsing platform: available at https:// www .iso .org/ obp
3.1
analytical technique
procedure whereby the concentration of the chemical is determined quantitatively
Note 1 to entry: These techniques are often specific to individual chemical and collection medium combinations.
Applicable techniques include, but are not limited to, flame ionization, photo ionization, electro-chemical, ultraviolet
and infrared spectrophotometry, gas and liquid chromatography, and colourimetry.
3.2
cleaning
process by which a garment is made again serviceable and/or hygienically wearable by removing any dirt or
contamination
[SOURCE: ISO 11610:2023, 4.6.16]
3.3
cumulative permeation mass
total amount of chemical that permeates during a specified time since the start of the test
[SOURCE: ISO 11610:2023, 7.5.15]
3.4
fumigant
pesticide in the form of gas
3.5
limited-use chemical protective garment
chemical protective garment for limited duration of use, i.e. to be worn until hygienic cleaning becomes
necessary or chemical contamination has occurred and disposal is required
Note 1 to entry: This includes protective clothing for single use and for limited re-use, according to the information
supplied by the manufacturer.
[SOURCE: ISO 11610:2023, 7.2.8, Note 2 to entry added]
3.6
partial body protective clothing
protective clothing that does not provide full-body coverage
Note 1 to entry: Partial body protective clothing may be used separately or in combination with other protective
clothing to increase the protection level of specific parts of the body. Examples applicable to this document include
items such as aprons, overshoes, sleeve protectors, material placed below knapsack/backpack sprayers, and smocks.
3.7
penetration
process by which a chemical moves through pores, apertures or other
openings in a material or finished garment on a non-molecular level
[SOURCE: ISO 11610:2023, 7.5.21]

ISO/DIS 27065:2025(en)
3.8
permeation
process by which a chemical moves through a protective clothing material on a molecular level
Note 1 to entry: The permeation involves sorption of the molecules of the chemical into the contacted (outside) surface
of a material, diffusion of the sorbed molecules in the material, and desorption of the molecules from the opposite
(inner) surface of the material.
Note 2 to entry: In this document, a chemical is a pesticide.
[SOURCE: ISO 11610:2023, 7.5.13, Note 2 to entry added]
3.9
pesticide
chemical compound that are used to control pests, including insects, rodents, fungi and unwanted plants (weeds)
Note 1 to entry: Pesticides are used in public health to control vectors of disease, such as mosquitoes, and in agriculture
to control pests that damage crops.
Note 2 to entry: For the purpose of this standard pesticides (plant protection products) are defined as chemicals used
in agricultural settings, green spaces, roadsides, etc.
Note 3 to entry: Pesticides (plant protection products) approved for use in one country may not be approved in another
country.
3.10
pesticide operator
person handling pesticides in agricultural settings
Note 1 to entry: Handling includes tasks such as mixing, loading, transferring, or applying pesticides; cleaning,
adjusting, or repairing the parts of mixing, loading, or application equipment that may contain pesticide residues;
assisting with the application of pesticides; and disposing of pesticides or pesticide containers.
Note 2 to entry: For the purpose of this standard, farm, forest, nursery, greenhouse, green spaces, and roadsides are
examples of agricultural settings.
3.11
protective clothing
clothing designed for the intended purpose of protecting all or part of the body from one or more
potential hazards
[SOURCE: ISO 11610:2023, 5.1.1, the term protecting replacing isolating]
3.12
protective garment material
any material or combination of materials used in an item of protective garment for the purpose of isolating
parts of the body from hazard
Note 1 to entry: For the purpose of this document, protective garment materials include those materials used in the
construction of whole or partial body protective garment that serve as the barrier for the wearer.
[SOURCE: ISO 11610:2023, 5.1.5, Note 1 to entry added]
3.13
re-entry worker
person who can be in contact with a plant protection product in an area that has previously been treated
3.14
repellency
characteristic to resist wetting and penetration by a liquid
[SOURCE: ISO 11610:2023, 7.5.1]

ISO/DIS 27065:2025(en)
3.15
re-usable chemical protective garment
chemical protective garment that is constructed from materials which allow the garment to be cleaned after
repeated chemical exposures such that it remains suitable for continued use
Note 1 to entry: For the purpose of this document chemical refers to pesticides, a sub-category of chemicals.
[SOURCE: ISO 11610:2023, 5.2.2, Note 1 to entry added]
3.16
structural seam
seam that is necessary for the integrity of the garment
Note 1 to entry: For the purpose of this document, a structural seam is a permanent joint between pieces of material
forming the protection against the penetration and/or the permeation of pesticides.
[SOURCE: ISO 11610:2023, 4.4.2, Note 1 to entry added]
3.17
test liquid
test chemical/ challenge chemical in form of liquid
[SOURCE: ISO 11610:2023, 7.5.3]
3.18
toxicity
ability of a substance at a specific dose to produce an adverse effect upon a living organism
4 Classification and testing requirements
All protective garments complying with this document shall meet the requirements of ISO 13688. The
protective garments shall be tested and classified by level of protection in accordance with the material,
seam, and whole-body protective garment requirements in Clauses 6, 7, and 8.
Level C1 protective clothing, including partial body: The materials and seams shall demonstrate a minimum
level of liquid penetration resistance. The protective clothing, including partial body, shall pass a practical
performance test. Level C1 can be used as the base protective garment with additional protective garments
based on risk assessment
Level C2 protective clothing, including partial body: The material and seams shall demonstrate a higher
level of liquid penetration resistance than Level C1 protective clothing. The protective garment, including
partial body, shall pass the practical performance test. The whole-body protective garment shall pass the
low-level spray test. It can be used as the base protective clothing in place of C1. with additional C3 partial
body garments based on risk assessment. It can be used as the base protective clothing in place of C1, with
additional C3 partial body garments based on risk assessment.
Level C3 protective clothing, including partial body: The materials and seams shall demonstrate a minimum
level of resistance to permeation. The protective garment, including partial body, shall pass the practical
performance test. The whole-body protective garment shall pass a high-level spray test. A Level C3 item is
suitable for use with concentrated as well as diluted pesticide formulations.
Table 1 provides a summary of the tests to be conducted for each level of protection. The detailed
requirements are found in the sub-clause and in Annex B (Table B.1). The stringency in testing requirements
to determine protection increases for each level. Therefore, any Level C2 protective clothing necessarily
meets Level C1 requirements and so does not need to be tested to achieve that level of protection. Similarly,
any Level C3 protective clothing necessarily meets Level C1 and Level C2 requirements. The strength
requirements are the same for all levels of protection, and the puncture resistance test is not mandatory.
If high puncture resistance is claimed, the item shall be tested as stated in Table 1 and the information

ISO/DIS 27065:2025(en)
supplied by the manufacturer shall include a statement informing the user that the item is suitable for
scenarios where puncture risk exists.
NOTE The puncture resistance test may provide beneficial information for purchasers selecting protective
garment, including partial body, for scenarios such as orchard spraying. It is not a mandatory requirement as puncture
resistance may not be important for other scenarios.
Table 1 — Summary requirements for Level C1, C2, and C3 protective clothing, including partial body
Levels
Sub-
Performance test
clause
C1 C2 C3
a
6.3 Material resistance to penetration (ISO 22608) x x
6.4 Material repellency (ISO 22608) x
b
6.5 Material resistance to permeation (ISO 19918) x
Material
Requirements
6.6 Tensile strength (ISO 13934-1) x x x
6.7 Tear resistance ISO 9073-4 x x x
c c c
6.8 Puncture resistance (ISO 13996) x x x
7.2 Seam penetration resistance (ISO 22608) x
Seam
b
7.3 Seam resistance to permeation (ISO 19918) x
requirements
7.4 Seam tensile strength (ISO 13935-2) x x x
8.1 Practical performance test (Annex A) x x x
Whole protective
d
garment 8.3.1 Low-level spray test (ISO 17491-4:2024 Method A) x
requirements
d
8.3.2 High-level spray test (ISO 17491-4:2024. Method B) x
a
The minimum performance requirement for Level C2 is significantly higher than that for Level C1 (see 6.2).
b
The permeation test is more severe than the penetration test. Therefore, material that meets 6.4 automatically meets the
6.2 penetration requirement. Also, if additional testing is required for a particular pesticide, the material shall also be tested for
permeation resistance using the pesticide in question.
c
Puncture resistance shall be tested if claimed by the manufacturer. EN 863 is equivalent to ISO 13996.
d
Not required for partial body protective garment.
5 Pre-treatment and conditioning
5.1 Pre-treatment by cleaning
Specimens used for each test specified in Clauses 6, 7, and 8 shall be pre-treated by cleaning. The cleaning
shall be in accordance with the manufacturer’s instructions on the basis of standardized processes. If the
manufacturer’s instructions indicate that cleaning is not allowed, i.e. limited-use protective garment, then
testing shall be carried out on new material.
Testing shall be carried out for the number of cleaning cycles for which the manufacturer guarantees the
performance. One cleaning cycle consists of one washing and one drying. Drying can be omitted if the
manufacturer recommends line drying. If the number of cleaning cycles is not specified, the tests shall be
carried out after 30 cleaning cycles. In all cases, the number of cycles, after which testing was conducted,
shall be included in accordance with Clause 10. If the manufacturer’s instructions indicate that both dry
cleaning and laundering are allowed, the test specimen shall undergo the laundering procedure only.
NOTE 1 Manufacturer’s instructions typically indicate one or several of the various methods and processes of
ISO 6330, ISO 15797, ISO 3175 or equivalent as standardized processes for cleaning.
If re-treatment is required, detailed instructions shall also be provided in accordance with Clause 10.
5.2 Conditioning
If the conditioning requirements are not indicated in the test method standard, all samples shall be
conditioned by storage at (23 ± 3) °C and (60 ± 10) % relative humidity for at least 24 h prior testing for

ISO/DIS 27065:2025(en)
clauses 6, 7, and 8. Testing of samples shall commence within 10 min of removing the sample from the
conditioning atmosphere.
5.3 Deterioration of repellency by cleaning
Where the finishing, applied to improve or maintain the repellency for liquids resistance performance of the
garment, can be deteriorated by the cleaning procedures indicated by the manufacturer, the manufacturer
shall indicate the maximum number of cleaning cycles that can be carried out before the garment needs to
be re-treated or re-impregnated to restore its performance.
Testing according to 6.2, 6.3, and 6.4 shall be carried out before the cleaning cycle for which the manufacturer
guarantees the performance.
For example, if the instructions state “re-impregnation during each third cleaning cycle”, tests shall be
performed after the second cleaning cycle, i.e. before re-impregnation. Then after re-impregnation is applied,
the test should be performed again in order to demonstrate meeting the requirement.
If the instructions state “re-impregnation after each cleaning cycle”, the tests shall be performed on new
items. Later, the item shall be cleaned, re-impregnation is applied and test repeated again in order to
demonstrate meeting the requirement.
In all cases, cleaning shall be according 5.1
6 Performance requirements of protective garment materials
6.1 General
Specimens for testing shall be taken from the original garment or from material or materials used in the
finished garment. The size, shape, and quantity shall be as required for each test procedure. If a material
is constructed of multiple layers, the specimen shall be cut with the order of each layer maintained and
tested with the outer layer on top. For protective garment constructed from different types of materials in
different body areas, each single material shall be tested separately.
For testing and classification of materials, all individual specimen results shall meet the performance class
requirement for the concerned property (see Annex B). The results shall be reported in accordance with
Clause 10.
Materials used for certain parts of the protective garment that provide higher performance (e.g. added
protection and/or strength) shall be tested if claimed by the manufacturer. The information regarding the
higher performing material, that may assist the purchaser in protective clothing selection, shall be included
in accordance with Clause 10 f).
NOTE For example, a protective clothing manufacturer can request permeation testing for the Level C2 protective
clothing to determine if the material will comply with Level C3 requirements.
6.2 Test chemical EC-DY preparation
The test chemical shall be diluted pesticide surrogate EC-DY, an emulsion of Disperse Yellow 26 (see Annex G)
in water. Water grade 3 (according to ISO 3696) or deionized water shall be used to prepare the diluted
solution with 2,5 % EC-DY. See Annex C concerning selection of test chemical and Annex G for composition of
the test chemical.
6.3 Material resistance to penetration (pipette test)
Materials for Level C1 and Level C2 protective clothing shall be tested in accordance with ISO 22608:2021,
Method A, using 0,2 ml of test chemical. Three specimens shall be tested for each material using the test
chemical in 6.2.
ISO/DIS 27065:2025(en)
For materials for protective garment classified as Level C1, the upper limit for percent penetration shall be
40,0 %. For materials classified as Level C2, the upper limit for percent penetration shall be 5,0 %.
In addition, materials for protective garment shall be tested for resistance to penetration according to
ISO 22608 Method B if one or more of the following requirements are met:
a) the test results for materials for protective garment classified as Level C1 are greater than 40,0 %
but less than 48,0 % (i.e. at least one specimen shows its result greater than 40,0 % and the three test
specimens show their result lower than 48,0 %).
b) the test results for materials for protective garment classified as Level C2 are greater than 5,0 %
but less than 6,0 % (i.e. at least one specimen shows its result greater than 5,0 % and the three test
specimens show their result lower than 6,0 %).If the specimens are tested with both methods A and B,
the results obtained by Method B shall be used for determining penetration classification and reporting
requirements.
NOTE 1 For Method A, a gravimetric method, the difference in the mass of the collector layer before and after
testing is used to calculate the % penetration. Method A is used in the first instance because this is the simpler and
less expensive of the two test methods. However, in some cases the increase in mass of the collector layer is due to
water and/or water vapour penetration. Yellow dye as surrogate serves as a tracer; stained area of the collector layer
can assist in visual analysis. For validation, Method B (chemical analysis) is required to confirm the amount of the
actual dye/active ingredient that has penetrated.
For materials for protective garment classified as Level C1, the three specimens tested shall have maximum
penetration of 40,0 %.
For materials for protective garment classified as Level C2, the three specimens tested shall have maximum
penetration of 5,0 %.
NOTE 2 ISO 22608 is an accelerated laboratory test that differentiates the penetration performance of materials
and seams. The maximum allowable penetration of 40,0 % is derived from the data analysis of cotton and cotton/
polyester protective garment used for operator exposure studies. The 40,0 % penetration limit for Level C1 protective
clothing is based on analysis of lab data as well as operator exposure study data. Studies conducted with the reference
fabric for Level C1 had less than 5,0 % penetration through the garment in operator exposure studies. Therefore, it is
not possible to substitute laboratory penetration data for field penetration data. For this reason, the laboratory test
data are used only to classify materials and seams. It cannot be used as default protection factors for risk assessment.
References [4] and [5] provide additional information on the analysis to establish the 40,0 % limit value for Level C1
and the 5,0 % limit value for Level C2.
NOTE 3 Test specimen with 64 cm circular opening can be used to prevent the test liquid from coming in contact
with the specimen holder when materials that have low absorption are used. Alternatively, the cell can be used with
an adequate sealing method to avoid the loss of the chemical at the edge.
6.4 Material Repellency (pipette test)
For Level C2 protective clothing, percent repellency shall also be calculated when ISO 22608:2021, Method
A, described in 6.2, is conducted to measure resistance to penetration.
Materials for protective garment classified as Level C2 shall have a minimum repellency of 80 % for all three
specimens tested.
6.5 Material resistance to permeation
For Level C3 protective clothing, three specimens shall be tested for cumulative permeation in accordance
with ISO 19918. Use(s) claimed by the manufacturer and stated in accordance with Clause 10 c) shall be used
as the basis for conducting the permeation test.
The test shall be conducted for 1 h using the test chemical in 6.2. Additional testing may be required to
determine cumulative permeation through material for specific pesticides. For additional testing, the
procedure, test methods, and pass criteria shall be the same as that for EC-DY stated above. The test chemical
shall be the specific pesticide formulation, either concentrated or diluted with water, in accordance with the
pesticide product labelling.
ISO/DIS 27065:2025(en)
Materials for protective garment classified as Level C3 shall have a maximum cumulative permeation of
10,0 µg/cm for all three specimens tested with pesticide surrogate EC-DY.
NOTE 1 The duration of the test is not based on actual use time since the permeation test is an accelerated test in
which the surface of the specimen is in constant contact with the test chemical. During field application with dilute
formulation, the duration of exposure may be for a longer period of time. However, the entire surface of a PPE item is
not in constant contact with the test chemical. During mixing and loading, contact with concentrates results only from
an accidental spill, in which case the operator is required to remove the protective garment immediately. Therefore,
the contact time with concentrates is much shorter.
NOTE 2 This accelerated test is used to classify materials, and therefore the 10,0 µg/cm maximum limit is not
appro
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