prEN 13708

SLOVENSKI STANDARD
oSIST prEN 13708:2019
01-december-2019

Živila - Detekcija obsevane hrane, ki vsebuje kristalni sladkor, s spektroskopijo

Produits alimentaires - Détection par spectroscopie RPE d’aliments ionisés contenant

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

Produits alimentaires - Détection par spectroscopie Lebensmittel - ESR-spektroskopischer Nachweis von

RPE d'aliments ionisés contenant des sucres bestrahlten Lebensmitteln, die kristallinen Zucker

This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations

which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other

language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,

Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,

Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without

© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 13708:2019 E

European foreword ....................................................................................................................................................... 3

1 Scope .................................................................................................................................................................... 4

2 Normative references .................................................................................................................................... 4

3 Terms and definitions ................................................................................................................................... 4

4 Principle ............................................................................................................................................................. 4

5 Apparatus and equipment ........................................................................................................................... 4

6 Procedure........................................................................................................................................................... 5

7 Evaluation .......................................................................................................................................................... 6

8 Limitations ......................................................................................................................................................... 7

9 Validation ........................................................................................................................................................... 7

10 Test report ......................................................................................................................................................... 8

Annex A (normative) Figures ................................................................................................................................... 9

Bibliography ................................................................................................................................................................. 10

This document (prEN 13708:2019) has been prepared by Technical Committee CEN/TC 275 “Food

This version published in 2001 has been changed by introduction of an information that this standard is

This document specifies a method for the detection of foods containing crystalline sugars which have

been treated with ionizing radiation, by analysing the electron spin resonance (ESR) spectrum, also

called electron paramagnetic resonance (EPR) spectrum, of the food, see [1] to [7].

Interlaboratory studies have been successfully carried out on dried figs, dried mangoes, dried papayas

ISO and IEC maintain terminological databases for use in standardization at the following addresses:

ESR spectroscopy detects paramagnetic centres (e.g. radicals). They are either due to irradiation or to

other compounds present. An intense external magnetic field produces a difference between the energy

levels of the electron spins m = + 1/2 and m = - 1/2, leading to resonance absorption of an applied

microwave beam in the spectrometer. ESR spectra are conventionally displayed as the first derivative of

The magnetic field and microwave frequency values depend on the experimental arrangements (sample

size and sample holder), while their ratio (i.e. g value) is an intrinsic characteristic of the paramagnetic

Radiation treatment produces radicals which can be detected in solid and dry parts of the food. The

intensity of the signal obtained increases with the concentration of the paramagnetic compounds and

including magnet, microwave bridge, console with field-controller and signal-channel, rectangular or

including frequency counter, magnetic field probe (nuclear magnetic resonance (NMR) Gaussmeter), or

Prepare suitable pieces (50 mg to 100 mg) of the fruit, e.g. using a scalpel. Avoid grinding of samples.

NOTE Various parts of the fruits can contain different quantities of crystalline sugars. It can be advantageous

Transfer a test portion directly into the ESR tube (3.3) and start the measurement.

Difficulties in tuning the spectrometer cavity can be experienced if the sample is insufficiently dry. In

this case either reduce the sample quantity or dry it further. Samples should be dried in a laboratory

Use a time constant and sweep rate appropriate for ESR signals with linewidths of 0,2 mT to 0,4 mT. For

example, the following ESR spectrometer settings have been found to be satisfactory:

1) Suprasil is an example of a suitable product available commercially. This information is only given for the

convenience of users of this International Standard and does not constitute an endorsement by CEN of