EN ISO 9233-2:2018

SLOVENSKI STANDARD
01-julij-2018
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SIST EN ISO 9233-2:2013
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Cheese, cheese rind and processed cheese - Determination of natamycin content - Part
2: High-performance liquid chromatographic method for cheese, cheese rind and
processed cheese (ISO 9233-2:2018)
Käse, Käserinde und Schmelzkäse - Bestimmung des Natamycingehalts - Teil 2:
Verfahren mit Hochleistungs-Flüssigchromatographie für Käse, Käserinde und
Schmelzkäse (ISO 9233-2:2018)
Fromage, croûte de fromage et fromages fondus - Détermination de la teneur en
natamycine - Partie 2: Méthode par chromatographie liquide à haute performance pour
fromage, croûte de fromage et fromages fondus (ISO 9233-2:2018)
Ta slovenski standard je istoveten z: EN ISO 9233-2:2018
ICS:
67.100.30 Sir Cheese
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 9233-2
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2018
EUROPÄISCHE NORM
ICS 67.100.30 Supersedes EN ISO 9233-2:2013
English Version
Cheese, cheese rind and processed cheese - Determination
of natamycin content - Part 2: High-performance liquid
chromatographic method for cheese, cheese rind and
processed cheese (ISO 9233-2:2018)
Fromage, croûte de fromage et fromages fondus - Käse, Käserinde und Schmelzkäse - Bestimmung des
Détermination de la teneur en natamycine - Partie 2: Natamycingehalts - Teil 2: Verfahren mit
Méthode par chromatographie liquide à haute Hochleistungs-Flüssigchromatographie für Käse,
performance pour fromage, croûte de fromage et Käserinde und Schmelzkäse (ISO 9233-2:2018)
fromages fondus (ISO 9233-2:2018)
This European Standard was approved by CEN on 4 April 2018.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2018 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 9233-2:2018 E
worldwide for CEN national Members.

Contents Page
European foreword . 3
European foreword
This document (EN ISO 9233-2:2018) has been prepared by Technical Committee ISO/TC 34 “Food
products” in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of
sampling and analysis” the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2018, and conflicting national standards
shall be withdrawn at the latest by November 2018.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 9233-2:2013.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta,
Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and the United Kingdom.
Endorsement notice
The text of ISO 9233-2:2018 has been approved by CEN as EN ISO 9233-2:2018 without any
modification.
INTERNATIONAL ISO
STANDARD 9233-2
IDF
140-2
Second edition
2018-03
Cheese, cheese rind and processed
cheese — Determination of natamycin
content —
Part 2:
High-performance liquid
chromatographic method for cheese,
cheese rind and processed cheese
Fromage, croûte de fromage et fromages fondus — Détermination de
la teneur en natamycine —
Partie 2: Méthode par chromatographie liquide à haute performance
pour fromage, croûte de fromage et fromages fondus
Reference numbers
ISO 9233-2:2018(E)
IDF 140-2:2018(E)
©
ISO and IDF 2018
ISO 9233-2:2018(E)
IDF 140-2:2018(E)
© ISO and IDF 2018
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office International Dairy Federation
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Published in Switzerland
ii © ISO and IDF 2018 – All rights reserved

ISO 9233-2:2018(E)
IDF 140-2:2018(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 2
6 Apparatus . 2
7 Sampling . 3
8 Preparation of test sample . 3
8.1 Cheese rind . 3
8.2 Cheese interior and processed cheese . 4
9 Procedure. 4
9.1 Test portion . 4
9.1.1 Cheese rind . 4
9.1.2 Cheese interior and processed cheese . 4
9.2 Preparation of test solution . 4
9.2.1 Cheese rind . 4
9.2.2 Cheese interior and processed cheese . 4
9.3 Determination . 5
9.3.1 Determination and detection limits . 5
9.3.2 Adjustment of the liquid chromatograph (6.14) . 5
9.3.3 Calibration graph . 5
9.3.4 Test solution. 5
9.3.5 Low natamycin content . 6
10 Calculation and expression of results . 6
10.1 Calculation of natamycin mass fraction . 6
10.2 Calculation of surface-area-related natamycin mass . 7
10.3 Correction of results . 7
10.4 Expression of results . 7
11 Precision . 7
11.1 Interlaboratory tests . 7
11.2 Repeatability . 7
11.3 Reproducibility . 7
12 Test report . 8
Annex A (informative) Examples . 9
Annex B (informative) Results of interlaboratory trial .11
Bibliography .12
ISO 9233-2:2018(E)
IDF 140-2:2018(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national
standards bodies (ISO member bodies). T
...