Plastics - Determination of the aerobic biodegradation of non-floating materials exposed to marine sediment - Method by analysis of evolved carbon dioxide (ISO 22404:2019)

This document specifies a laboratory test method to determine the degree and rate of aerobic biodegradation level of plastic materials. This test method can also be applied to other materials.
Biodegradation is determined by measuring the CO2 evolved by the plastic material when exposed to marine sediments sampled from a sandy tidal zone and kept wet with salt-water under laboratory conditions.
This test method is a simulation under laboratory conditions of the habitat found in sandy tidal zone that, in marine science, is called eulittoral zone.
The conditions described in this document might not always correspond to the optimum conditions for the maximum degree of biodegradation to occur.
Deviations from the test conditions described in this document are justified in the test report.

Kunststoffe - Bestimmung des aeroben Bioabbaus von nicht-schwimmenden Materialien, die marinem Sediment ausgesetzt sind - Verfahren mittels Analyse des freigesetzten Kohlenstoffdioxids (ISO 22404:2019)

Dieses Dokument legt ein Laborprüfverfahren für die Bestimmung des Grads und der Geschindigkeit des aeroben Bioabbaus von Kunststoffmaterialien fest. Dieses Prüfverfahren kann darüber hinaus auch auf andere Materialien angewendet werden.
Bioabbau wird durch die Messung des durch das Kunststoffmaterial erzeugten CO2 bestimmt, wenn das Material Meeressediment ausgesetzt war, das einer sandigen Gezeitenzone entnommen und mit Salzwasser unter Laborbedingungen aufbewahrt wurde.
Dieses Prüfverfahren ist eine Simulation unter Laborbedingungen des in der sandigen Gezeitenzone ange¬troffenen Habitats, das in der Meereswissenschaft als eulitorale Zone bezeichnet wird.
Die in diesem Dokument beschriebenen Bedingungen entsprechen möglicherweise nicht immer den Optimal¬bedingungen, unter denen der höchste Grad an biologischem Abbau möglich ist.
Abweichungen von den in diesem Dokument beschriebenen Prüfbedingungen werden in dem Prüfbericht begründet.

Plastiques - Détermination de la biodégradation aérobie des matériaux non flottants exposés aux sédiments marins - Méthode par analyse du dioxyde de carbone libéré (ISO 22404:2019)

Le présent document spécifie une méthode d’essai en laboratoire permettant de déterminer le taux et le niveau de biodégradation aérobie des matériaux plastiques. Cette méthode d’essai peut également être appliquée à d’autres matériaux.
La biodégradation est déterminée en mesurant le CO2 libéré par le matériau plastique lorsqu’il est exposé à des sédiments marins prélevés dans une zone tidale sableuse et maintenus humides dans de l’eau salée, dans des conditions de laboratoire.
Cette méthode d’essai est une simulation, dans des conditions de laboratoire, de l’habitat présent dans la zone tidale sableuse, connue sous le nom de «zone eulittorale» en sciences marines.
Les conditions décrites dans le présent document ne correspondent pas nécessairement aux conditions optimales permettant d’obtenir le taux maximal de biodégradation.
Les écarts par rapport aux conditions d’essai décrites dans le présent document sont justifiés dans le rapport d’essai.

Polimerni materiali - Določanje aerobne biorazgradljivosti neplavajočih materialov, izpostavljenih morskim sedimentom - Metoda z analizo sproščenega ogljikovega dioksida (ISO 22404:2019)

Ta dokument določa laboratorijsko preskusno metodo za določevanje stopnje in hitrosti aerobne biorazgradnje polimernih materialov. To preskusno metodo je mogoče uporabiti tudi za druge materiale.
Biorazgradljivost se določi z merjenjem CO2, ki se sprošča iz polimernega materiala ob izpostavljenosti morskim sedimentom, vzorčenim iz peščenega območja plimovanja in vlaženim s slano vodo pod laboratorijskimi pogoji.
Ta preskusna metoda je simulacija habitata v peščenem območju plimovanja, ki se v morski znanosti imenuje eulitoralno območje, pod laboratorijskimi pogoji.
Pogoji, ki so opisani v tem dokumentu, morda niso vedno enaki idealnim pogojem, pri katerih pride do največje stopnje biorazgradnje.
Odstopanja od preskusnih pogojev, opisanih v tem dokumentu, so utemeljena v poročilu o preskusu.

General Information

Status
Published
Publication Date
09-Nov-2021
Withdrawal Date
30-May-2022
Technical Committee
Drafting Committee
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
10-Nov-2021
Due Date
21-Aug-2023
Completion Date
10-Nov-2021

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SLOVENSKI STANDARD
01-januar-2022
Polimerni materiali - Določanje aerobne biorazgradljivosti neplavajočih materialov,
izpostavljenih morskim sedimentom - Metoda z analizo sproščenega ogljikovega
dioksida (ISO 22404:2019)
Plastics - Determination of the aerobic biodegradation of non-floating materials exposed
to marine sediment - Method by analysis of evolved carbon dioxide (ISO 22404:2019)
Kunststoffe - Bestimmung des aeroben Bioabbaus von nicht-schwimmenden Materialien,
die marinem Sediment ausgesetzt sind - Verfahren mittels Analyse des freigesetzten
Kohlenstoffdioxids (ISO 22404:2019)
Plastiques - Détermination de la biodégradation aérobie des matériaux non flottants
exposés aux sédiments marins - Méthode par analyse du dioxyde de carbone libéré
(ISO 22404:2019)
Ta slovenski standard je istoveten z: EN ISO 22404:2021
ICS:
13.020.40 Onesnaževanje, nadzor nad Pollution, pollution control
onesnaževanjem in and conservation
ohranjanje
83.080.01 Polimerni materiali na Plastics in general
splošno
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 22404
EUROPEAN STANDARD
NORME EUROPÉENNE
November 2021
EUROPÄISCHE NORM
ICS 83.080.01
English Version
Plastics - Determination of the aerobic biodegradation of
non-floating materials exposed to marine sediment -
Method by analysis of evolved carbon dioxide (ISO
22404:2019)
Plastiques - Détermination de la biodégradation Kunststoffe - Bestimmung des aeroben Bioabbaus von
aérobie des matériaux non flottants exposés aux nicht-schwimmenden Materialien, die marinem
sédiments marins - Méthode par analyse du dioxyde de Sediment ausgesetzt sind - Verfahren mittels Analyse
carbone libéré (ISO 22404:2019) des freigesetzten Kohlenstoffdioxids (ISO 22404:2019)
This European Standard was approved by CEN on 8 November 2021.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2021 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 22404:2021 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
The text of ISO 22404:2019 has been prepared by Technical Committee ISO/TC 61 "Plastics” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 22404:2021 by
Technical Committee CEN/TC 249 “Plastics” the secretariat of which is held by NBN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by May 2022, and conflicting national standards shall be
withdrawn at the latest by May 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards body.
A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 22404:2019 has been approved by CEN as EN ISO 22404:2021 without any modification.

INTERNATIONAL ISO
STANDARD 22404
First edition
2019-09
Plastics — Determination of the
aerobic biodegradation of non-
floating materials exposed to marine
sediment — Method by analysis of
evolved carbon dioxide
Plastiques — Détermination de la biodégradation aérobie des
matériaux non flottants exposés aux sédiments marins — Méthode
par analyse du dioxyde de carbone libéré
Reference number
ISO 22404:2019(E)
©
ISO 2019
ISO 22404:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Fax: +41 22 749 09 47
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 22404:2019(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Test environment . 2
6 Reagents . 2
7 Apparatus . 3
8 Procedure. 3
8.1 Test material . 3
8.2 Reference material . 4
8.3 Preparation of the sediment . 4
8.4 Test setup . 4
8.5 Pre-conditioning phase . 4
8.6 Start of the test . 5
8.7 Carbon dioxide measurement . 5
8.8 End of the test . 6
9 Calculation and expression of results . 6
9.1 Calculation . 6
9.1.1 Amount of CO produced . 6
9.1.2 Percentage of biodegradation. 8
9.2 Expression and interpretation of results . 8
10 Validity of results . 9
11 Test report . 9
Bibliography .10
ISO 22404:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www .iso .org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 61, Plastics, Subcommittee SC 14,
Environmental aspects.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved

ISO 22404:2019(E)
Introduction
Products made with biodegradable plastics and other biodegradable mat
...

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