ASTM D8375-23

This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: D8375 − 23
Standard Test Method for
Determination of Cannabinoid Concentration in Dried
Cannabis and Hemp Raw Materials using Liquid
1
Chromatography Tandem Mass Spectrometry (LC-MS/MS)
This standard is issued under the fixed designation D8375; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
NOTE 1—For this test method, dried raw material from a cannabis plant
1. Scope
includes one or more of inflorescence, leaves, or stems.
1.1 This test method allows for the concentration determi-
NOTE 2—Certain jurisdictions or regulations may require specific parts
nation of the cannabinoids listed in Table 1, and shall apply to of the plant to be included or excluded for analysis and those regulations
will take precedence for the selection of plant parts.
any dried raw material from a cannabis plant (Note 1, Note 2)
regardless of the type of cannabis plant from which it was
1.2 Units—The values stated in SI units are to be regarded
2
derived. For the sake of brevity, the term “cannabis” shall be
as standard. No other units of measurement are included in this
used from now on to refer to any type of cannabis plant
standard.
including those that can be classified as hemp. The procedure
1.3 List of Measurable Analytes—See Table 1.
includes sub-sampling a ground, homogeneous sample, extrac-
3,4
1.4 This standard does not purport to address all of the
tion with methanol:water (80:20, v:v), dilution in methanol
safety concerns, if any, associated with its use. It is the
and analysis by liquid chromatography tandem mass spectrom-
responsibility of the user of this standard to establish appro-
etry (LC-MS/MS). The method allows for a wide-range of
priate safety, health, and environmental practices and deter-
sample concentrations to be determined by using a 1000-fold
mine the applicability of regulatory limitations prior to use.
calibration range and the option to perform multiple levels of
1.5 This international standard was developed in accor-
sample dilution. The calibration curve is prepared in methanol
dance with internationally recognized principles on standard-
over a range of 10 ng ⁄mL to 10 000 ng/mL for all seventeen
ization established in the Decision on Principles for the
cannabinoids, or a subset of cannabinoids if desired, while the
Development of International Standards, Guides and Recom-
sample extracts are diluted in methanol into the calibration
3,4,5
mendations issued by the World Trade Organization Technical
range. For example, a 1/500 dilution of sample extracts
Barriers to Trade (TBT) Committee.
allows concentration determination over a range of 0.5 mg ⁄g to
500 mg ⁄g in cannabis. The method was validated with quality
2. Referenced Documents
control samples prepared in methanol, a candidate certified
6
2.1 ASTM Standards:
reference material (CRM), and repeat extraction and analysis
3
D1193 Specification for Reagent Water
of cannabinoid samples.
D8245 Guide for Disposal of Resin-Containing Cannabis
Raw Materials and Downstream Products
1 D8270 Terminology Relating to Cannabis
This test method is under the jurisdiction of ASTM Committee D37 on
D8282 Practice for Laboratory Test Method Validation and
Cannabis and is the direct responsibility of Subcommittee D37.03 on Laboratory.
Current edition approved March 1, 2023. Published March 2023. Originally
Method Development
approved in 2022. Last previous edition approved in 2022 as D8375 – 22. DOI:
E203 Test Method for Water Using Volumetric Karl Fischer
10.1520/D8375-23.
2 Titration
Health Canada, Guidance Document: Good production practices guide for
cannabis Testing for Phytocannabinoids.
3
McRae, G. and Melanson, J. E., Quantitative determination and validation of 17
3. Terminology
cannabinoids in cannabis and hemp using liquid chromatography-tandem mass
3.1 Definitions—For general terms related to cannabis, refer
spectrometry, Anal Bioanal Chem, Vol 412, No. 27, 2020, pp. 7381–7393,
doi:10.1007/s00216-020-02862-8.
to Terminology D8270.
4
Mudge, E. M., Murch, S. J., Brown, P. N., Leaner and greener analysis of
3.2 Definitions of Terms Specific to This Standard:
cannabinoids, Anal Bioanal Chem, Vol 409, No. 12, 2017, pp. 3153–3163, doi:
10.1007/s00216-017-0256-3.
5
Vaclavik, L., Benes, F., Fenclova, M., Hricko, J., Krmela, A., Svobodova, V., et
6
al. Quantitation of cannabinoids in cannabis dried plant materials, concentrates, and For referenced ASTM standards, visit the ASTM website, www.as
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