ASTM D4200-82(2003)

NOTICE: This standard has either been superseded and replaced by a new version or withdrawn.
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Designation:D4200– 82 (Reapproved 2003)
Standard Test Method for
Evaluating Inhibitory Effects of Ink Grids on Membrane
Filters
This standard is issued under the fixed designation D4200; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
1. Scope riological culture techniques for the detection and enumeration
of bacterial organisms.
1.1 Thistestmethoddescribesaprocedurewherebytheuser
5.2 Aparticularly sensitive organism and growth conditions
of ink-gridded membrane filters in water quality studies can
have been selected for this test method in order to maximize
ascertain whether or not the grid lines are toxic and inhibitory
sensitivity to toxic materials possibly present in the inks used
to bacterial growth when the membrane and its entrapped
for grid-marking membrane filters.
bacteria are incubated on a suitable media.
1.2 This standard does not purport to address all of the
6. Apparatus
safety concerns, if any, associated with its use. It is the
6.1 Incubator, capable of maintaining temperatures of
responsibility of the user of this standard to establish appro-
44.56 0.2°C.
priate safety and health practices and determine the applica-
6.2 Membrane Filtration Units.
bility of regulatory limitations prior to use.
6.3 Vacuum Source with trap vessel.
2. Referenced Documents 6.4 Forceps, blunt-nosed.
6.5 Autoclave or other sterilizing equipment.
2.1 ASTM Standards:
6.6 Expendables:
D1129 Terminology Relating to Water
6.6.1 Gridded membrane filters.
D1193 Specification for Reagent Water
6.6.2 1-mL and 10-mL pipets.
3. Terminology
6.6.3 Petri dishes (50-mm) containing 6 to 8 mL of agar
medium or a 100-mm dish with 20 6 2 mLof agar medium, or
3.1 Definitions—For definitions of terms used in this test
both.
method, refer to Terminology D1129.
6.6.4 Erlenmeyer flasks.
4. Summary of Test Method
7. Reagents and Materials
4.1 A heavy bacterial suspension is filtered through a
7.1 Purity of Water—Unless otherwise indicated, reference
gridded membrane filter.The bacterial concentration employed
to water shall be understood to mean reagent water conforming
is sufficient to cover much of the membrane with bacterial
to reagent water Type II of Specification D1193.
colonies.
7.2 M-FC Agar with Rosolic Acid or equivalent (henceforth
4.2 After filtration the membrane is incubated on a suitable
referred to as agar medium), formulated, prepared, and dis-
medium and the distribution of the colonies and shape of the
pensed in accordance with the manufacturer’s specifications.
colonies noted in the area around each grid line.
7.3 Tryptone Soya Broth or equivalent (henceforth referred
5. Significance and Use
to as broth medium), formulated, prepared, and dispensed in
accordance with the manufacturer’s specifications.
5.1 This test method may be applied to determine the
7.4 Peptone Water, 0.1 %, sterile.
suitability of grid-marked membrane filters for use in bacte-
7.5 Broth Culture of E. coliATCC11229,18-h,preparedas
follows: Add 1 mL of an 18 6 2-h broth culture of E. coli
This test method is under the jurisdiction of ASTM Committee D19 on Water
ATCC 11229 to 99 mL of 0.1 % peptone water, mix thor-
and
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