ASTM E979-20
(Practice)Standard Practice for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
Standard Practice for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
SIGNIFICANCE AND USE
5.1 This procedure is designed to determine the effectiveness of antimicrobial agents intended for microbial control in invert emulsions and other water containing hydraulic fluids.
SCOPE
1.1 This laboratory practice is designed to evaluate the utility and effectiveness of antimicrobial agents intended to control microbial growth in invert emulsions and other water containing hydraulic fluids.
Note 1: Procedures for preparation of water soluble hydraulic fluids and recovery of organisms appear in Practice E2169.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
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This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the
Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
Designation: E979 − 20
Standard Practice for
Evaluation of Antimicrobial Agents as Preservatives for
Invert Emulsion and Other Water Containing Hydraulic
1
Fluids
This standard is issued under the fixed designation E979; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision.Anumber in parentheses indicates the year of last reapproval.A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Invert emulsion hydraulic fluids typically contain 60% mineral oil and 40% water (by volume).
These fluids routinely are prepared using proprietary, oil-soluble, emulsifying agents, as well as other
emulsifiable constituents. They are recommended for use where conditions indicate a low-cost, fire
retardant product, compatible with water-based metal working fluids.
The high water content of these hydraulic fluids makes them susceptible to microbial attack.
Uncontrolled microbial growth in these fluids can cause cartridge filter unit plugging, maladorous
conditions, or general biodeterioration. Problem microorganisms associated with these fluids include
bacteria and fungi.
Thehydraulicsystemisessentiallyaclosedoneinwhichwaterofevaporationisaddedtomaintain
a fixed volume. The inclusion of an efficacious preservative in the water containing hydraulic fluids
can prevent microbial growth and the resulting problems that follow.
1. Scope* mendations issued by the World Trade Organization Technical
Barriers to Trade (TBT) Committee.
1.1 This laboratory practice is designed to evaluate the
utility and effectiveness of antimicrobial agents intended to
2. Referenced Documents
control microbial growth in invert emulsions and other water
containing hydraulic fluids. 2
2.1 ASTM Standards:
D1129Terminology Relating to Water
NOTE 1—Procedures for preparation of water soluble hydraulic fluids
and recovery of organisms appear in Practice E2169.
D4454Test Method for Simultaneous Enumeration of Total
andRespiringBacteriainAquaticSystemsbyMicroscopy
1.2 The values stated in SI units are to be regarded as
3
(Withdrawn 2015)
standard. No other units of measurement are included in this
standard. D5465Practices for Determining Microbial Colony Counts
from Waters Analyzed by Plating Methods
1.3 This standard does not purport to address all of the
E1326Guide for Evaluating Non-culture Microbiological
safety concerns, if any, associated with its use. It is the
Tests
responsibility of the user of this standard to establish appro-
E2169Practice for Selecting Antimicrobial Pesticides for
priate safety, health, and environmental practices and deter-
Use in Water-Miscible Metalworking Fluids
mine the applicability of regulatory limitations prior to use.
E2523Terminology for Metalworking Fluids and Opera-
1.4 This international standard was developed in accor-
tions
dance with internationally recognized principles on standard-
E2694Test Method for Measurement ofAdenosineTriphos-
ization established in the Decision on Principles for the
Development of International Standards, Guides and Recom- phate in Water-Miscible Metalworking Fluids
1 2
This practice is under the jurisdiction ofASTM Committee E35 on Pesticides, For referenced ASTM standards, visit the ASTM website, www.astm.org, or
Antimicrobials, and Alternative Control Agents and is the direct responsibility of contact ASTM Customer Service at service@astm.org. For Annual Book of ASTM
Subcommittee E35.15 on Antimicrobial Agents. Standards volume information, refer to the standard’s Document Summary page on
Current edition approved Dec. 1, 2020. Published December 2020. Originally the ASTM website.
3
approvedin1984.Lastpreviouseditionapprovedin2015asE979–09(2015).DOI: The last approved version of this historical standard is referenced on
10.1520/E0979-20. www.astm.org.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E979 − 20
3. Terminology 6.6 Simulated Filters:
1
6.6.1 Strainer, 3-in. epoxy coated, ⁄4-in. mesh gutter
3.1 Terms used in this practice are defined inTerminologies
4
strainer.
D1129 and E2523.
6.6.2 Screen, 16 by 18 in. fiberglass screening material.
4. Summary of Test Method
NOTE 4—Fiberglass mesh screening material (16 by 18 in.) is available
from any local hardware dealer.
4.1 The antimicrobial agent to be evaluated is incorporated
into an emulsion system by (a) addition to the aqueous phase
6.6.3 Wire, 20-gauge, galvanized or stainless steel.
employedinthepre
...
This document is not an ASTM standard and is intended only to provide the user of an ASTM standard an indication of what changes have been made to the previous version. Because
it may not be technically possible to adequately depict all changes accurately, ASTM recommends that users consult prior editions as appropriate. In all cases only the current version
of the standard as published by ASTM is to be considered the official document.
Designation: E979 − 09 (Reapproved 2015) E979 − 20
Standard Practice for
Evaluation of Antimicrobial Agents as Preservatives for
Invert Emulsion and Other Water Containing Hydraulic
1
Fluids
This standard is issued under the fixed designation E979; the number immediately following the designation indicates the year of
original adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the year of last reapproval. A
superscript epsilon (´) indicates an editorial change since the last revision or reapproval.
INTRODUCTION
Invert emulsion hydraulic fluids typically contain 60 % mineral oil and 40 % water (by volume).
These fluids routinely are prepared using proprietary, oil-soluble, emulsifying agents, as well as other
emulsifiable constituents. They are recommended for use where conditions indicate a low-cost, fire
retardant product, compatible with water-based metal working fluids.
The high water content of these hydraulic fluids makes them susceptible to microbial attack.
Uncontrolled microbial growth in these fluids can cause cartridge filter unit plugging, maladorous
conditions, or general biodeterioration. Problem microorganisms associated with these fluids include
bacteria and fungi.
The hydraulic system is essentially a closed one in which water of evaporation is added to maintain
a fixed volume. The inclusion of an efficacious preservative in the water containing hydraulic fluids
can prevent microbial growth and the resulting problems that follow.
1. Scope*
1.1 This laboratory practice is designed to evaluate the utility and effectiveness of antimicrobial agents intended to control
microbial growth in invert emulsions and other water containing hydraulic fluids.
NOTE 1—Procedures for preparation of water soluble hydraulic fluids and recovery of organisms appear in Practice E2169.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility
of the user of this standard to establish appropriate safety safety, health, and healthenvironmental practices and determine the
applicability of regulatory limitations prior to use.
1.4 This international standard was developed in accordance with internationally recognized principles on standardization
established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued
by the World Trade Organization Technical Barriers to Trade (TBT) Committee.
1
This practice is under the jurisdiction of ASTM Committee E35 on Pesticides, Antimicrobials, and Alternative Control Agents and is the direct responsibility of
Subcommittee E35.15 on Antimicrobial Agents.
Current edition approved Oct. 1, 2015Dec. 1, 2020. Published February 2016December 2020. Originally approved in 1984. Last previous edition approved in 20092015
as E979 – 09.E979 – 09(2015). DOI: 10.1520/E0979-09R15.10.1520/E0979-20.
*A Summary of Changes section appears at the end of this standard
Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States
1
---------------------- Page: 1 ----------------------
E979 − 20
2. Referenced Documents
2
2.1 ASTM Standards:
D1129 Terminology Relating to Water
D4454 Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
3
(Withdrawn 2015)
D5465 Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
E1326 Guide for Evaluating Non-culture Microbiological Tests
E2169 Practice for Selecting Antimicrobial Pesticides for Use in Water-Miscible Metalworking Fluids
E2523 Terminology for Metalworking Fluids and Operations
E2694 Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids
3. Terminology
3.1 Terms used in this practice are defined in Terminologies D1129 and E2523.
4. Summary of Test Method
4.1 The antimicrobial agent to be evaluated is incorporated into an emulsion system by (a) addition to the aqueous phase employed
in the preparation of the emulsion, (b) in doses to the formulated system, or (c) by other methods suitable for the test compound.
4.2 A heavy bacterial or fungal inoculum, or both, is then added.
4.3 The resulting mixture is aerated and passed over the surface of a simulated filter system for a minimum period of eight weeks
either continuously or with shutdowns to simulate actual o
...
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