SIST EN ISO 21253-2:2020

SLOVENSKI STANDARD
01-januar-2020
Kakovost vode - Metode za več spojin - 2. del: Merila za kvantitativno določevanje
organskih spojin z analizno metodo za več spojin (ISO 21253-2:2019)
Water quality - Multi-compound class methods - Part 2: Criteria for the quantitative
determination of organic substances using a multi-compound class analytical method
(ISO 21253-2:2019)
Wasserbeschaffenheit - Gemeinsam erfassbare Stoffgruppen - Teil 2: Kriterien für die
Beurteilung eines Multianalyt-Verfahrens organischer Stoffe (ISO 21253-2:2019)
Qualité de l'eau - Méthodes d'analyse de composés multi-classes - Partie 2: Critères
pour la détermination quantitative de composés organiques avec une méthode d'analyse
de composés multi-classes (ISO 21253-2:2019)
Ta slovenski standard je istoveten z: EN ISO 21253-2:2019
ICS:
13.060.50 Preiskava vode na kemične Examination of water for
snovi chemical substances
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 21253-2
EUROPEAN STANDARD
NORME EUROPÉENNE
October 2019
EUROPÄISCHE NORM
ICS 13.060.50
English Version
Water quality - Multi-compound class methods - Part 2:
Criteria for the quantitative determination of organic
substances using a multi-compound class analytical
method (ISO 21253-2:2019)
Qualité de l'eau - Méthodes d'analyse de composés Wasserbeschaffenheit - Gemeinsam erfassbare
multi-classes - Partie 2: Critères pour la détermination Stoffgruppen - Teil 2: Kriterien für die Beurteilung
quantitative de composés organiques avec une eines Multianalyt-Verfahrens organischer Stoffe (ISO
méthode d'analyse de composés multi-classes (ISO 21253-2:2019)
21253-2:2019)
This European Standard was approved by CEN on 9 August 2019.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

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United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2019 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 21253-2:2019 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 21253-2:2019) has been prepared by Technical Committee ISO/TC 147 "Water
quality" in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of
which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by April 2020, and conflicting national standards shall be
withdrawn at the latest by April 2020.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 21253-2:2019 has been approved by CEN as EN ISO 21253-2:2019 without any
modification.
INTERNATIONAL ISO
STANDARD 21253-2
First edition
2019-08
Water quality — Multi-compound
class methods —
Part 2:
Criteria for the quantitative
determination of organic substances
using a multi-compound class
analytical method
Qualité de l'eau — Méthodes d'analyse de composés multi-classes —
Partie 2: Critères pour la détermination quantitative de composés
organiques avec une méthode d'analyse de composés multi-classes
Reference number
ISO 21253-2:2019(E)
©
ISO 2019
ISO 21253-2:2019(E)
© ISO 2019
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
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Published in Switzerland
ii © ISO 2019 – All rights reserved

ISO 21253-2:2019(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 3
5 Selection of the matrix . 3
6 Sample preservation prior to analysis. 4
7 Internal standards and injection standards . 4
7.1 General . 4
7.2 Selection of standards . 4
8 Calibration . 5
9 Recovery determination . 6
9.1 General . 6
9.2 Quantification by external calibration . 6
9.3 Quantification by internal calibration . 7
10 Limit of quantification (LOQ) . 7
11 Results . 7
11.1 Identification of the compounds . 7
11.2 Quantification . 7
11.3 Measurement uncertainty . 7
12 Quality controls . 7
12.1 General . 7
12.2 Quality control checks on the blank . 8
12.3 Quality control checks on the internal standards . 8
12.4 Quality control checks on the limit of quantification. 8
Annex A (informative) Matrix effect . 9
Bibliography .10
ISO 21253-2:2019(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www .iso .org/iso/foreword .html.
This document was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 2,
Physical, chemical and biochemical methods.
A list of all parts in the ISO 21253 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
iv © ISO 2019 – All rights reserved

INTERNATIONAL STANDARD ISO 21253-2:2019(E)
Water quality — Multi-compound class methods —
Part 2:
Criteria for the quantitative determination of organic
substances using a multi-compound class analytical method
1 Scope
This document specifies the criteria for developing an in-house mass spectrometry-based method for
quantitative analysis of multiple subgroups of organic substances in the scope of physical-chemical
analysis of water.
This document supplements ISO/TS 13530 which provides guidance on the initial characterization of
the measurement performances, by providing details to select the test matrix and internal standards
and criteria for analyte and internal standard recoveries.
This document is not intended as a substitute for the currently applicable analytical standards
dedicated to organic compounds but as a resource bringing additional characterization elements.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 8466-1, Water quality — Calibration and evaluation of analytical methods and estimation of
performance characteristics — Part 1: Statistical evaluation of the linear calibration function
ISO 8466-2, Water quality — Calibration and evaluation of analytical methods and estimation of
performance characteristics — Part 2: Calibration strategy for non-linear second-order calibration
functions
ISO 11352, Water quality — Estimation of measurement uncertainty based on validation and quality
control data
ISO 21253-1, Water quality — Multi- compound class methods — Part 1: Criteria for the identification of
target compounds by gas and liquid chromatography and mass spectrometry
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https: //www .iso .org ./obp
— IEC Electropedia: available at http: //www .electropedia .org/
3.1
analyte
substance to be determined
[SOURCE: ISO/TS 28581:2012, 3.1]
ISO 21253-2:2019(E)
3.2
blank
aliquot of reagent water (reagent blank) or of a matrix in which the analyte (3.1) is absent (matrix blank)
that is treated exactly as a sample through the complete analytical procedure including extraction,
clean-up, identification and quantification including all the relevant reagents and materials
Note 1 to entry: It is crucial that the laboratory specifies which blank is considered.
3.3
calibration curve
expression of the relation between indication and corresponding measured quantity value
[SOURCE: ISO/IEC Guide 99:2007, 4.31]
3.4
limit of quantification
LOQ
lowest value of a determinand that can be determined with an acceptable level of accuracy, which could
be estimated by different means and shall be verified in the intended matrix
[SOURCE: ISO/TS 21231:2019, 3.2.5, modified — Note to entry has been excluded.]
3.5
analytical method
unambiguously written procedure describing all details required to carry out the analysis of the
determinand or parameter, namely: scope and field of application, principle and/or reactions,
definitions, reagents, apparatus, analytical procedures, calculations and presentation of results,
performance data and test report
[SOURCE: ISO/TS 16489:2006, 3.3]
3.6
recovery
relative recovery
extent to which a known, added quantity of determinand in a sample can be measured by an
analytical system
Note 1 to entry: Recovery is calculated from the difference between results obtained from a spiked and an
unspiked aliquot of sample and is usually expressed as a percentage.
[SOURCE: ISO 5667-14:2014, 3.8]
3.7
relative retention time
ratio between the retention time of the target compound and the retention time of the calibration
standard
[SOURCE: ISO 15680:2003, 3.5, modified — "retention-time standard" has been replaced by "calibration
standard"]
3.8
injection standard
standard mixture added to a sample before injection into the GC-MS apparatus, to monitor variability
of instrument response and to calculate internal standard recovery
Note 1 to entry: The same definition is applied for GC-MS/MS, LC-MS and LC-MS/MS.
[SOURCE: ISO 28540:2011, 3.4, modified — Note to entry has been replaced.]
2 © ISO 2019 – All rights reserved

ISO 21253-2:2019(E)
3.9
subgroup of compounds
analytes (3.1) pre-classified into groups that share similar patterns of analytical behaviour in response
to extraction mode and analysis
3.10
internal standard
compound added in a known amount to the sample from the beginning of the protocol and enabling
analytical coverage throughout the procedure, and that is used to correct for losses during sample
preparation and analysis by accounting for all-system matrix effects (recoveries, ionization effect,
variability of the detector response of the instrument for example)
Note 1 to entry: Determining the ratio of characteristic molecule signal intensity to the internal standard makes
it possible to calculate the quantitative ratio between molecule and internal standard and thereby to deduce
molecule concentration in
...